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Fuzhuanbrick tea (FBT) has attracted wide attention because of its substantial nutritional value. This article first studied the protective mechanism of FBT polysaccharide (FBTP) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) by 16S rDNA amplicon sequencing technology and metabonomics. It was demonstrated that the administration of FBTP by oral gavage (100, 200, and 400 mg/kg·bw) could decrease the disease activity index (DAI), prevent colon shortening, and alleviate colon tissue damage and inflammation in mice with UC. Interestingly, FBTP relieved the intestinal microbiota disorder caused by UC and contributed to the proliferation of beneficial microbiota, such as Lactobacillus and Akkermansia, followed by a significant increase in the levels of short-chain fatty acids (SCFAs). Furthermore, FBTP dramatically altered tryptophan metabolism and elevated the fecal contents of indole-3-acetaldehyde (IAld) and indole-3-acetic acid (IAA). It was also found that FBTP significantly increased the colonic expressions of aromatic hydrocarbon receptors (AhR) and interleukin-22 (IL-22) and further promoted the expressions of intestinal tight junction (TJ) proteins ZO-1 and occludin in the colitis mice. Cumulatively, these findings suggest that FBTP can relieve UC by regulating intestinal flora disorders, promoting microbial metabolism, and repairing the intestinal barrier.

Strawberry (Fragaria chiloensis) is a major edible berry with various potential health benefits. This study determined the protective effects of whole strawberry (WS) against dextran-sulfate-sodium-induced colitis in mice. In colitic mice, dietary WS reduced the disease activity index, prevented the colon shortening and spleen enlargement, and alleviated the colonic tissue damages. The abundance of proinflammatory immune cells was reduced by dietary WS in the colonic mucosa, which was accompanied by the suppression of overproduction of proinflammatory cytokines. Western blotting and immunohistochemical analysis revealed that dietary WS decreased the expression of proinflammatory proteins in the colonic mucosa. Moreover, dietary WS partially reversed the alteration of gut microbiota in the colitic mice by increasing the abundance of potential beneficial bacteria, e.g., Bifidobacterium and Lactobacillus, and decreasing the abundance of potential harmful bacteria, e.g., Dorea and Bilophila. Dietary WS also restored the decreased production of short-chain fatty acids in the cecum of the colitic mice. The results revealed the anti-inflammatory effects and mechanisms of dietary WS in the colon, which is critical for the rational utilization of strawberry for the prevention of inflammation-driven diseases.

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