Chemical characteristics combined with bioactivity for comprehensive evaluation of Panax ginseng C.A. Meyer in different ages and seasons based on HPLC-DAD and chemometric methods

Shan, Si‐Ming; Luo, Jian‐Guang; Huang, Fang; Kong, Ling–Yi

doi:10.1016/j.jpba.2013.10.030

Cited by 65 publications

(54 citation statements)

References 29 publications

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“…The establishment of fingerprint-activity relationships allows assessment of the quality of different batches in a holistic way to predict activity based on fingerprint data and to predict the constituents most highly correlated with bioactivity [125][126][127].…”

Section: Considerations On Quality Control and Standardization Of Hermentioning

confidence: 99%

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

Pferschy‐Wenzig

Bauer

2015

Epilepsy & Behavior

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Section: Considerations On Quality Control and Standardization Of Hermentioning

confidence: 99%

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

Pferschy‐Wenzig

Bauer

2015

Epilepsy & Behavior

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“…Moreover, it is economically critical to evaluate and control the quality of ginseng roots in the food industry and herbal markets. One of the most important issues is to evaluate the growth age of ginseng roots, and several analytical methods have been developed for this purpose [7,8,9,10]. In particular, qualitative and quantitative ginsenoside contents have been studied widely in ginseng roots at different ages [11,12].…”

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry Based Profiling and Imaging of Various Ginsenosides from Panax ginseng Roots at Different Ages

Lee

et al. 2017

IJMS

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(1) Background: Panax ginseng root is one of the most important herbal products, and the profiling of ginsenosides is critical for the quality control of ginseng roots at different ages in the herbal markets. Furthermore, interest in assessing the contents as well as the localization of biological compounds has been growing. The objective of this study is to carry out the mass spectrometry (MS)-based profiling and imaging of ginsenosides to assess ginseng roots at different ages; (2) Methods: Optimal ultra performance liquid chromatography coupled to quadrupole time of flight/MS (UPLC-QTOF/MS) was used to profile various ginsenosides from P. ginseng roots. Matrix-assisted laser desorption ionization (MALDI)-time of flight (TOF)/MS-based imaging was also optimized to visualize ginsenosides in ginseng roots; (3) Results: UPLC-QTOF/MS was used to profile 30 ginsenosides with high mass accuracy, with an in-house library constructed for the fast and exact identification of ginsenosides. Using this method, the levels of 14 ginsenosides were assessed in P. ginseng roots cultivated for 4, 5, and 6 years. The optimal MALDI-imaging MS (IMS) was also applied to visualize the 14 ginsenosides in ginseng roots. As a result, the MSI cross sections showed the localization of 4 ginsenoside ions ([M + K]+) in P. ginseng roots at different ages; (4) Conclusions: The contents and localization of various ginsenosides differ depending on the cultivation years of P. ginseng roots. Furthermore, this study demonstrated the utility of MS-based profiling and imaging of ginsenosides for the quality control of ginseng roots.

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“…Recently, the chromatographic fingerprints combined with chemometrics methods have been widely used for the analysis and quality control of medicine (Avula et al ., , ; Chen et al ., , ; Shan et al ., ; Yu et al ., ). Chen et al .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the chromatographic fingerprints combined with chemometrics methods have been widely used for the analysis and quality control of medicine (Avula et al, 2013(Avula et al, , 2014Chen et al, 2008Chen et al, , 2013aShan et al, 2014;Yu et al, 2013). Chen et al (2008) developed an HPLC-UV method combined with fingerprints and multivariate analysis for quality control and original discrimination of Ganoderma lucidum (Leyss.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of six index constituents and comparative analysis of four ethnomedicines from genus Gentiana using a UPLC‐UV‐MS method

Pan

Shen

Zhang

et al. 2014

Biomedical Chromatography

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Many species from genus Gentiana (Gentianaceae) have a long history of applications as folk medicines in the world. A simple rapid UPLC-UV-MS/MS method has been developed and validated for the simultaneous determination of six index constituents (gentiopicroside, swertiamarin, loganic acid, sweroside, mangiferin and ferulic acid) from the four ethnomedicines (G. rigescens Franch. ex Hemsl., G. rhodantha Franch. ex Hemsl., G. scabra Bunge and G. farreri Balf. f.). The UPLC analysis was performed on Shim-Pack XR-ODS III (150 × 2.0 mm, 2.2 µm). The mobile phase was consisted of acetonitrile-0.1% formic acid water using gradient elution. The wavelength 242 nm was chose for the four iridoids as well as mangiferin and 320 nm was set for ferulic acid. Mass spectrometry was applied for identification and quantification for analytes with low concentration. All the regression equations revealed a good linear relationship (R(2) > 0.9993). Accuracy and precision were all within the required limits. The chromatogram fingerprints analysis combined with principal component analysis showed the similarity values of the four species were <0.788 while the similarity values of G. scabra Bunge and G. rigescens Franch. ex Hemsl. were >0.993, which provided reasonable foundation for utilization and clinical application of the four ethnomedicines. This developed method appears to be a useful tool for quality control of the four ethnomedicines.

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Chemical characteristics combined with bioactivity for comprehensive evaluation of Panax ginseng C.A. Meyer in different ages and seasons based on HPLC-DAD and chemometric methods

Cited by 65 publications

References 29 publications

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

Mass Spectrometry Based Profiling and Imaging of Various Ginsenosides from Panax ginseng Roots at Different Ages

Simultaneous determination of six index constituents and comparative analysis of four ethnomedicines from genus Gentiana using a UPLC‐UV‐MS method

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