Chemical and morphological changes on platinum microelectrode surfaces in AC and DC fields with biological buffer solutions

Gencoglu, Aytug; Minerick, Adrienne R.

doi:10.1039/b820126a

Cited by 44 publications

(41 citation statements)

References 26 publications

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“…These electrode channels are separated from the main channel by thin insulating barriers. The absence of metal electrodes minimizes sample contamination 35 and bubble formation, and resolves issues associated with joule heating. 36,37 We have shown previously that the viability of prostate cancer cells did not change through characterization or isolation by cDEP.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

et al. 2012

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Ovarian cancer is the leading cause of death from gynecological malignancies in women. The primary challenge is the detection of the cancer at an early stage, since this drastically increases the survival rate. In this study we investigated the dielectrophoretic responses of progressive stages of mouse ovarian surface epithelial (MOSE) cells, as well as mouse fibroblast and macrophage cell lines, utilizing contactless dielectrophoresis (cDEP). cDEP is a relatively new cell manipulation technique that has addressed some of the challenges of conventional dielectrophoretic methods. To evaluate our microfluidic device performance, we computationally studied the effects of altering various geometrical parameters, such as the size and arrangement of insulating structures, on dielectrophoretic and drag forces. We found that the trapping voltage of MOSE cells increases as the cells progress from a nontumorigenic, benign cell to a tumorigenic, malignant phenotype. Additionally, all MOSE cells display unique behavior compared to fibroblasts and macrophages, representing normal and inflammatory cells found in the peritoneal fluid. Based on these findings, we predict that cDEP can be utilized for isolation of ovarian cancer cells from peritoneal fluid as an early cancer detection tool. V C 2012 American Institute of Physics. [http://dx

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Section: Introductionmentioning

confidence: 99%

Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

et al. 2012

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“…At those conditions, real-time optical microscopy reveals continuous bubble formation that leads to irreversible damage of the electrodes. The phenomenon is related to solvent electrolysis and hydrogen gas formation, which produce erosion and atom migration [38][39]. The strong current increase measured between both electrodes gives rise to a significant reduction of the parallel resistance.…”

Section: In-situ Characterization Of the Nw Dispersionmentioning

confidence: 99%

“…Furthermore, although several studies have been performed to evaluate the effects of AC voltage and frequency on the DEP alignment, it is a challenge to control the trapping rate only through those parameters because of local variations of the NW concentration over the alignment sites. In addition, large voltages can induce solvent electrolysis whose effects are bubble formation and electrode degradation [38][39]. Real-time monitoring can help to detect those anomalies as soon as they happen, allowing fast correction of the alignment parameters.…”

Section: Introductionmentioning

confidence: 99%

Continuous-flow system and monitoring tools for the dielectrophoretic integration of nanowires in light sensor arrays

et al. 2015

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“…It can, if a high enough potential is applied (>1 V vs. Ag/AgCl) and oxygen is present in the electrolyte, form an oxide layer on the surface. 30 This process can even continue to oxide dissolution 33 and etching as described in Paper 1.…”

Section: Electrodesmentioning

confidence: 99%

Electrodes and Electrokinetic Systems for Biotechnological Applications

Nilsson¹

2015

Linköping Studies in Science and Technology. Dissertations

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Research in bioelectronics studies biological systems and materials in combination with electronic interfaces for the development of devices, e.g., for medical applications, drug and toxicity tests, and biotechnology in general. Neural implants and pacemakers are examples of products developed from this area of research. Conducting polymers such as poly(3,4-ethylenedioxythiophene) (PEDOT) bridge biology and electronics with a combination of biocompatibility, flexibility, and capability to themselves undergo redox reactions. Electrokinetics, a related branch of science, describes the motion of fluids and particles caused by the application of an electric field, and includes various separation techniques such as gel electrophoresis. Applying an electric field in a sufficiently small diameter silica capillary can cause the liquid in the capillary to move. This phenomenon, referred to as electroosmosis, plays an important role in miniaturized microfluidic systems and can be used to drive flow in a so-called electroosmotic pump.This thesis describes research at the interface between biology, chemistry and electronics. The first two papers probe the adsorption mechanism of poly-L-lysine, often used in biotechnological applications, onto hard materials such as metals (platinum) and metal oxides (indium tin oxide). By employing a gravimetric technique, quartz crystal microgravimetry with dissipation monitoring (QCM-D) combined with electrochemistry, we studied the process by which poly-L-lysine is deposited onto two different conducting substrates under anodic conditions. We found that indium tin oxide is more suitable than platinum for anodic electrodeposition of PLL, however, the exact film deposition mechanism is not fully understood. Paper 3 demonstrates the applicability of using conducting polymers, (e.g., PEDOT) instead of platinum as electrode material in gel electrophoresis. The last paper describes the fabrication and characterization of an electroosmotic pump consisting of a potassium silicate stationary phase in a fused silica capillary and the integration of the pump into a system for use, e.g., as a bioreactor. Populärvetenskaplig sammanfattningBiologi och elektronik har historiskt varit två separata forskningsområden som i nuläget till stor del även kombineras inom fältet bioelektronik. Ett viktigt exempel inom bioelektroniken är utvecklingen av pacemakern under 1950-talet. Andra exempel på applikationer är bl. a. biosensorer, neurologiska implantat och miniatyriserade analyssystem, s.k. Lab-On-a-Chip. Inom bioelektronik syftar man till att studera och påverka biologiska system och material med elektroteknik. År 2000 gavs Nobelpriset i kemi till Heeger, MacDiarmid och Shirakawa för deras utveckling av elektriskt ledande polymerer samt möjligheten att öka dess ledningsförmåga genom en process som kallas dopning. Konjugerade polymerer såsom poly(3,4-ethylenedioxythiophene) (PEDOT) är viktiga verktyg för att binda samman biologi med elektronik genom deras flexibilitet, biokompatibilitet samt förmågan...

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Chemical and morphological changes on platinum microelectrode surfaces in AC and DC fields with biological buffer solutions

Cited by 44 publications

References 26 publications

Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

Continuous-flow system and monitoring tools for the dielectrophoretic integration of nanowires in light sensor arrays

Electrodes and Electrokinetic Systems for Biotechnological Applications

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