Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

Salmanzadeh, Alireza; Kittur, Harsha; Sano, Michael B.; Roberts, Paul C.; Schmelz, Eva M.; Davalos, Rafael V.

doi:10.1063/1.3699973

Cited by 55 publications

(66 citation statements)

References 55 publications

(84 reference statements)

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“…4 Addressing the challenge of isolating ovarian cancer cells from peritoneal fluid, we previously demonstrated that a microfluidic approach based on exploitation of cell electrical properties could be useful. 30 We reported that MOSE cell stages ranging from benign to malignant undergo complete trapping at different voltages in the frequency range of 200-600 kHz. 30 The current paper expands the work from our previous study by further investigating the differences in the electrical properties of each cell stage of the MOSE model.…”

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confidence: 99%

Investigating dielectric properties of different stages of syngeneic murine ovarian cancer cells

et al. 2013

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In this study, the electrical properties of four different stages of mouse ovarian surface epithelial (MOSE) cells were investigated using contactless dielectrophoresis (cDEP). This study expands the work from our previous report describing for the first time the crossover frequency and cell specific membrane capacitance of different stages of cancer cells that are derived from the same cell line. The specific membrane capacitance increased as the stage of malignancy advanced from 15.39 6 1.54 mF m À2 for a non-malignant benign stage to 26.42 6 1.22 mF m À2 for the most aggressive stage. These differences could be the result of morphological variations due to changes in the cytoskeleton structure, specifically the decrease of the level of actin filaments in the cytoskeleton structure of the transformed MOSE cells. Studying the electrical properties of MOSE cells provides important information as a first step to develop cancer-treatment techniques which could partially reverse the cytoskeleton disorganization of malignant cells to a morphology more similar to that of benign cells. V C 2013 American Institute of Physics.

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Section: -13mentioning

confidence: 99%

Investigating dielectric properties of different stages of syngeneic murine ovarian cancer cells

et al. 2013

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“…22 Contactless DEP microfluidic device was utilized to study the behavior of mouse ovarian cells. 23 Human cervical carcinoma cell line HeLa was concentrated using circular microelectrodes. 24 DEP-based printed circuit boards have been used for software controlled entrapment and movement of human tumor cells.…”

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ApoStream™, a new dielectrophoretic device for antibody independent isolation and recovery of viable cancer cells from blood

et al. 2012

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Isolation and enumeration of circulating tumor cells (CTCs) are used to monitor metastatic disease progression and guide cancer therapy. However, currently available technologies are limited to cells expressing specific cell surface markers, such as epithelial cell adhesion molecule (EpCAM) or have limited specificity because they are based on cell size alone. We developed a device, ApoStream TM that overcomes these limitations by exploiting differences in the biophysical characteristics between cancer cells and normal, healthy blood cells to capture CTCs using dielectrophoretic technology in a microfluidic flow chamber. Further, the system overcomes throughput limitations by operating in continuous mode for efficient isolation and enrichment of CTCs from blood. The performance of the device was optimized using a design of experiment approach for key operating parameters such as frequency, voltage and flow rates, and buffer formulations. Cell spiking studies were conducted using SKOV3 or MDA-MB-231 cell lines that have a high and low expression level of EpCAM, respectively, to demonstrate linearity and precision of recovery independent of EpCAM receptor levels. The average recovery of SKOV3 and MDA-MB-231 cancer cells spiked into approximately 12 Â 10 6 peripheral blood mononuclear cells obtained from 7.5 ml normal human donor blood was 75.4% 6 3.1% (n ¼ 12) and 71.2% 6 1.6% (n ¼ 6), respectively. The intra-day and inter-day precision coefficients of variation of the device were both less than 3%. Linear regression analysis yielded a correlation coefficient (R

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“…The presented technique has been successfully used for a variety of more complex and physiologically relevant applications, including sorting live and dead cells, 28 tumor initiating cells from prostate cancer cells, 30 cancer cells from dilute red blood cells, 31,32 and differentiating among stages of breast cancer 37 and ovarian cancer. 29 cDEP has also been used for mixing particles. 38 These applications suggest that by using the simple technique presented, diverse purposes can be accomplished simply by altering the design of the channel geometry.…”

Section: 36mentioning

confidence: 99%

“…Higher frequency devices (100-600 kHz) have operated using pDEP and achieved batch sorting of cells, such as prostate tumor initiating cells (TICs), murine ovarian surface epithelial (MOSE) cells, MDA-MB-231 breast cancer cells, or live THP-1 cells by selectively trapping cells of interest on insulating posts located in the sample channel. [28][29][30][31] Lower frequency (5-100 kHz) devices operate continuously, and when operated at a frequency at which one population experiences pDEP while the background population experiences nDEP, can redirect particle trajectories to achieve sorting. [32][33][34] These low frequency devices have been used to sort cancer cells from red blood cells, determine the changes in dielectric properties of a progressive MOSE cell line, and to elucidate the effects of non-toxic sphingolipid treatments on reversing aggressive characteristics of aggressive MOSE cells.…”

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confidence: 99%

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Elvington

Salmanzadeh

Stremler

et al. 2013

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Dielectrophoresis (DEP) is the phenomenon by which polarized particles in a non-uniform electric field undergo translational motion, and can be used to direct the motion of microparticles in a surface marker-independent manner. Traditionally, DEP devices include planar metallic electrodes patterned in the sample channel. This approach can be expensive and requires a specialized cleanroom environment. Recently, a contact-free approach called contactless dielectrophoresis (cDEP) has been developed. This method utilizes the classic principle of DEP while avoiding direct contact between electrodes and sample by patterning fluidic electrodes and a sample channel from a single polydimethylsiloxane (PDMS) substrate, and has application as a rapid microfluidic strategy designed to sort and enrich microparticles. Unique to this method is that the electric field is generated via fluidic electrode channels containing a highly conductive fluid, which are separated from the sample channel by a thin insulating barrier. Because metal electrodes do not directly contact the sample, electrolysis, electrode delamination, and sample contamination are avoided. Additionally, this enables an inexpensive and simple fabrication process. cDEP is thus well-suited for manipulating sensitive biological particles. The dielectrophoretic force acting upon the particles depends not only upon spatial gradients of the electric field generated by customizable design of the device geometry, but the intrinsic biophysical properties of the cell. As such, cDEP is a label-free technique that avoids depending upon surface-expressed molecular biomarkers that may be variably expressed within a population, while still allowing characterization, enrichment, and sorting of bioparticles.Here, we demonstrate the basics of fabrication and experimentation using cDEP. We explain the simple preparation of a cDEP chip using soft lithography techniques. We discuss the experimental procedure for characterizing crossover frequency of a particle or cell, the frequency at which the dielectrophoretic force is zero. Finally, we demonstrate the use of this technique for sorting a mixture of ovarian cancer cells and fluorescing microspheres (beads).

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Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

Cited by 55 publications

References 55 publications

Investigating dielectric properties of different stages of syngeneic murine ovarian cancer cells

Investigating dielectric properties of different stages of syngeneic murine ovarian cancer cells

ApoStream™, a new dielectrophoretic device for antibody independent isolation and recovery of viable cancer cells from blood

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

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