Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation

Chou, Chun-Hsu; Sung, Tzu-Jung; Hu, Yu‐Ning; Lu, Hsin-Yu; Yang, Li-Chan; Cheng, Kuan‐Chen; Lai, Ping‐Shan; Hsieh, Chang‐Wei

doi:10.1016/j.ijbiomac.2019.03.154

Cited by 47 publications

(35 citation statements)

References 56 publications

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“…Even though these substances have a beneficial effect on the wound healing process, overproduction H2O2 gives rise to the non-healing wound via weakening wound-healing related cell, fibroblast [17,44]. In our previous study, we proved that pre-treated with PNPs reduced the H2O2-induced damage to L929 cell using MTT assay, suggesting PNPs might protect L929 cell from programmed cell death by ameliorating oxidative stress in L929 cell [16]. To assess if PNPs possess the ability against H2O2-induced oxidative stress, we utilized ROS generation assay as a model.…”

Section: Effect Of Intracellular Ros Generationmentioning

confidence: 83%

“…To assess if PNPs possess the ability against H 2 O 2-induced oxidative stress, we utilized ROS generation assay as a model. We had already proved that PNPs did not exhibit cytotoxicity toward L929 cells under a concentration of 500 µg/mL [16]. The levels of ROS, determined through DCFH-DA staining, produced in control cells, H 2 O 2 -induced cells without PNPs pretreatment and with PNPs pretreatment (500 µg/mL) are shown in Figure 4a; in this figure, green fluorescence indicates ROS production.…”

Section: Effect Of Intracellular Ros Generationmentioning

confidence: 90%

“…Our results show that among the three fractions, PNP-80 had the greatest antioxidant activity. Moreover, although PNPs did not exhibit antioxidant activity as good as vitamin C did, PNPs possess a variety of wound healing-related functions, such as moisturizing [16] which vitamin did not hold.…”

Section: Reducing Power and Hydroxyl Radical Scavenging Activitymentioning

confidence: 91%

“…PNPs were prepared as described in our previous study [16]. PNPs were extracted using ethanol precipitation.…”

Section: Extraction and Fractionationmentioning

confidence: 99%

“…Collagenases are a group of zinc-containing proteinases, which contains Zn ion at its active site that facilitates interaction with an inhibitor [43]. Previous studies showed that PNPs have the ability to chelate metal [16], which might suggest that the inhibitory mechanism of PNPs against collagenase is due to their ability to chelate Zn ion at collagenase's active site and hamper the interaction between the substrate and its active site. Low-molecular-weight polysaccharides have a less compact structure than do high-molecular-weight polysaccharides.…”

Section: Anti-collagenase Activity Of Pnpsmentioning

confidence: 99%

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Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

et al. 2020

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Pholiota nameko, a type of edible and medicinal fungus, is currently grown extensively for food and traditional medicine in China and Japan. It possesses various biological activities, such as anti-inflammatory, anti-hyperlipidemia and antitumor activities. However, P. nameko has rarely been discussed in the field of dermatology; identifying its biological activities could be beneficial in development of a new natural ingredient used in wound care. To evaluate its in vitro wound healing activities, the present study assessed the antioxidant and anti-collagenase activities of P. nameko polysaccharides (PNPs) prepared through fractional precipitation (40%, 60% and 80% (v/v)); the assessments were conducted using reducing power, hydroxyl radical scavenging activity, dichloro-dihydro-fluorescein diacetate and collagenase activity assays. The ability of PNPs to facilitate L929 fibroblast cell proliferation and migration was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and scratch assays. The findings indicated that, among all fractions, PNP-80 showed the best antioxidant and anti-collagenase activity, as measured by their reducing power (IC50 of PNP-80 was 2.43 ± 0.17 mg/mL), the hydroxyl radical scavenging (IC50 of PNP-80 was 2.74 ± 0.11 mg/mL) and collagenase activity assay, and significantly reduced cellular ROS content, compared with that of H2O2-induced L929 cells. Moreover, PNP-80 significantly promoted L929 fibroblast proliferation and migration, compared with the control group. Overall, we suggested that PNP-80 could be a promising candidate for further evaluation of its potential application on wound healing.

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Section: Effect Of Intracellular Ros Generationmentioning

confidence: 83%

Section: Effect Of Intracellular Ros Generationmentioning

confidence: 90%

Section: Reducing Power and Hydroxyl Radical Scavenging Activitymentioning

confidence: 91%

“…PNPs were prepared as described in our previous study [16]. PNPs were extracted using ethanol precipitation.…”

Section: Extraction and Fractionationmentioning

confidence: 99%

Section: Anti-collagenase Activity Of Pnpsmentioning

confidence: 99%