A mutant of seawater Arthrospira platensis with high polysaccharides production induced by space environment and its application potential

Chen, Zishuo; Li, Tan; Yang, Bingjie; Wu, Jiayi; Li, Tao; Wu, Houbo; Wu, Hao; Xiang, Wenzhou

doi:10.1016/j.algal.2021.102562

Cited by 6 publications

(6 citation statements)

References 57 publications

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“…Furthermore, the EMS-D mutant shifted its carbon partitioning into carbohydrates and lipids, whereas the EMS-R mutant preferred carbohydrates as the primary carbon sink. Conversion of proteins to carbohydrates has also been reported in Arthrospira platenis mutants . The observed difference in the biochemical composition of the mutants in the present study, particularly in lipid content, was higher in flask cultures than in PBR cultures (Figures B and B).…”

Section: Resultsmentioning

confidence: 99%

“…Conversion of proteins to carbohydrates has also been reported in Arthrospira platenis mutants. 18 The observed difference in the biochemical composition of the mutants in the present study, particularly in lipid content, was higher in flask cultures than in PBR cultures (Figures 1B and 2B). This could be attributed to nutritional stress in flasks, which triggered neutral lipid biosynthesis, whereas the high nitrogen and phosphorus starting concentrations in the PBR prevented such stress from occurring.…”

Section: Growth Performance and Biochemicalmentioning

confidence: 89%

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Dissecting Enhanced Carbohydrate and Pigment Productivity in Mutants of Nannochloropsis oculata Using Metabolomics and Lipidomics

Arora

Philippidis

2022

J. Agric. Food Chem.

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Random mutagenesis is an effective strategy for enhancing cellular traits. In this study, we used the mutagen ethyl methanesulfonate to create fast-growing Nannochloropsis oculata mutants. When cultivated in a photobioreactor with a diel cycle, two mutants exhibited 2.2-fold higher carbohydrate productivity and 3.5–4.0-fold higher pigment productivity than the wild type, while one of them also showed 2.5-fold higher lipid productivity. A comprehensive physiological, metabolomic, and lipidomic study showed that the mutants had high levels of glucose-, galactose-, and xylose-based carbohydrates. Their high growth rate was attributed to increased chlorophyll a content, improved nitrogen assimilation, storage, and recycling, and low monogalactosyldiacyl glycerol/digalactosyldiacyl glycerol ratio, which was responsible for higher biomass productivity. The investigation revealed upregulation of lipid precursors, shedding light on high lipid accumulation. The derived algae strains are capable of increasing the biosynthesis of value-added storage molecules without impairing growth, rendering them promising candidates for commercial development in future biorefineries.

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Section: Resultsmentioning

confidence: 99%

Section: Growth Performance and Biochemicalmentioning

confidence: 89%

Dissecting Enhanced Carbohydrate and Pigment Productivity in Mutants of Nannochloropsis oculata Using Metabolomics and Lipidomics

Arora

Philippidis

2022

J. Agric. Food Chem.

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“…Structural polysaccharides mainly refer to fiber polysaccharides in the cell walls of eukaryotic microalgae, outer layer peptidoglycans in prokaryotic cyanobacteria, and some extracellular polysaccharides in exosomes (Chen, Tan, Yang, et al, 2022). Despite their relatively low abundance, structural polysaccharides are characterized by rich diversity in terms of type and serve as primary contributors to the maintenance of cell morphology and rigidity in microalgae.…”

Section: Polysaccharidesmentioning

confidence: 99%

The potential use of microalgae for nutrient supply and health enhancement in isolated and confined environments

Zhang,

Yang,

Sun

et al. 2024

Comp Rev Food Sci Food Safe

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Exploring isolated and confined environments (IACEs), such as deep‐sea ecosystems, polar regions, and outer space, presents multiple challenges. Among these challenges, ensuring sustainable food supply over long timescales and maintaining the health of personnel are fundamental issues that must be addressed. Microalgae, as a novel food resource, possess favorable physiological and nutritional characteristics, demonstrating potential as nutritional support in IACEs. In this review, we discuss the potential of microalgae as a nutritional supplement in IACEs from four perspectives. The first section provides a theoretical foundation by reviewing the environmental adaptability and previous studies in IACEs. Subsequently, the typical nutritional components of microalgae and their bioavailability are comprehensively elucidated. And then focus on the impact of these ingredients on health enhancement and elucidate its mechanisms in IACEs. Combining the outstanding stress resistance, rich active ingredients, the potential to alleviate osteoporosis, regulate metabolism, and promote mental well‐being, microalgae demonstrate significant value for food applications. Furthermore, the development of novel microalgae biomatrices enhances health safeguards. Nevertheless, the widespread application of microalgae in IACEs still requires extensive studies and more fundamental data, necessitating further exploration into improving bioavailability, high biomass cultivation methods, and enhancing palatability.

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“…More recently, a new technique was developed, known as Space Mutation Breeding (SMB). This technique may have direct or indirect effects on the growth and metabolic activities of microalgae, due to the unusual environment of space, characterized by high-energy ionic radiation, space’s magnetic field, ultra-high vacuum and microgravity [ 121 ]. The SMB technique provides some advantages, such as the great improvement in species’ qualities in a short time [ 122 ].…”

Section: Further Approaches In Random Mutagenesismentioning

confidence: 99%

“…The SMB technique provides some advantages, such as the great improvement in species’ qualities in a short time [ 122 ]. This was achieved by Chen Zishuo et al [ 121 ], with a seawater Arthrospira platensis mutant, yielding a sugar content 62.26% higher than the wild type.…”

Section: Further Approaches In Random Mutagenesismentioning

confidence: 99%

Strain Development in Microalgal Biotechnology—Random Mutagenesis Techniques

Bleisch

Freitag

Ihadjadene

et al. 2022

Life

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Microalgal biomass and metabolites can be used as a renewable source of nutrition, pharmaceuticals and energy to maintain or improve the quality of human life. Microalgae’s high volumetric productivity and low impact on the environment make them a promising raw material in terms of both ecology and economics. To optimize biotechnological processes with microalgae, improving the productivity and robustness of the cell factories is a major step towards economically viable bioprocesses. This review provides an overview of random mutagenesis techniques that are applied to microalgal cell factories, with a particular focus on physical and chemical mutagens, mutagenesis conditions and mutant characteristics.

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A mutant of seawater Arthrospira platensis with high polysaccharides production induced by space environment and its application potential

Cited by 6 publications

References 57 publications

Dissecting Enhanced Carbohydrate and Pigment Productivity in Mutants of Nannochloropsis oculata Using Metabolomics and Lipidomics

Dissecting Enhanced Carbohydrate and Pigment Productivity in Mutants of Nannochloropsis oculata Using Metabolomics and Lipidomics

The potential use of microalgae for nutrient supply and health enhancement in isolated and confined environments

Strain Development in Microalgal Biotechnology—Random Mutagenesis Techniques

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