Cheers: Cracking Open The Bottleneck of Extraction in Bioanalysis

Breadmore, Michael C.

doi:10.4155/bio.15.212

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…When the analytes reach the sample BGE interface, they experience a low field strength and they will dramatically slow down and “stack.” The sensitivity enhancement in FASS is determined by the ratio of velocities in the sample and the BGE zones. The enhancement in detection sensitivity is usually around 10–20 times when compared to hydrodynamic injection (HDI) of a sample with an equivalent conductivity to that of the BGE . Despite of its simplicity and ease of applicability, FASS suffers from two major shortcomings.…”

Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Breadmore

Wuethrich

et al. 2016

Electrophoresis

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One of the most cited limitations of capillary (and microchip) electrophoresis is the poor sensitivity. This review continues to update this series of biennial reviews, first published in Electrophoresis in 2007, on developments in the field of on-line/in-line concentration methods in capillaries and microchips, covering the period July 2014-June 2016. It includes developments in the field of stacking, covering all methods from field amplified sample stacking and large volume sample stacking, through to isotachophoresis, dynamic pH junction, and sweeping. Attention is also given to on-line or in-line extraction methods that have been used for electrophoresis.

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Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Breadmore

Wuethrich

et al. 2016

Electrophoresis

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Selective Nanoparticles in Microextraction

Ríos-Gómez

Lasarte‐Aragonés

Cárdenas

et al. 2016

Encyclopedia of Analytical Chemistry

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Sorption capacity is a critical issue in microextraction techniques where the amount of sorbent is reduced to the low milligram range and even lower. In this context, the use of sorbents with a high superficial area is really desirable. Nanoparticles (NPs) fulfill this main requirement while, at the same time, provide the analyst with a wide variety of interaction chemistries that can be easily selected depending on the analytical problem under study. In addition, certain NPs present especial properties (e.g. superparamagnetism) that make them so attractive in the microextraction context. Focusing on sorption capacity is meaningless when complex samples, the usual situation in Bioanalysis, are processed as matrix components may overload the sorbent avoiding the extraction of the target analytes. In this scenario, selectivity then becomes a critical variable of concern. This article discusses the role of selectivity in microextraction and provides a general overview of the main coatings used to boost the selectivity of NPs. In particular, this article describes the use of two groups of coatings as selectivity enhancers. On the one hand, the use of synthetic polymers including molecularly imprinted polymers (MIPs) and restricted access materials (RAMs) is a well‐established alternative in analytical sciences. On the other hand, biopolymers, such as antibodies and aptamers, exploit biorecognition that is by far the most selective interaction. Both alternatives are described in detail in this article.

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Cheers: Cracking Open The Bottleneck of Extraction in Bioanalysis

Cited by 2 publications

References 14 publications

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Selective Nanoparticles in Microextraction

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