Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Breadmore, Michael C.; Wuethrich, Alain; Li, Feng; Phung, Sui Ching; Kalsoom, Umme; Cabot, Joan M.; Tehranirokh, Masoomeh; Shallan, Aliaa I.; Keyon, Aemi Syazwani Abdul; See, Hong Heng; Dawod, Mohamed; Quirino, Joselito P.

doi:10.1002/elps.201600331

Cited by 88 publications

(43 citation statements)

References 209 publications

(333 reference statements)

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“…During the reviewed period, no other methodological review on electrophoretic sample stacking appeared; the below mentioned reviews have broader or narrower scopes, or are focused on specific aspects. General reviews were aimed at enrichment techniques and enhancement of sensitivity in CE , their application in chiral CE , in CE‐MS and in the analysis of alkaloids . Electrophoretic stacking was also included in specialized reviews on CE analyses of peptides and proteins , pharmaceuticals , pesticides , antibiotics , food , and biologically important thiols .…”

Section: Introductionmentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

2018

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Electrophoretic sample stacking comprises a group of capillary electrophoretic techniques where trace analytes from the sample are concentrated into a short zone (stack). This paper is a continuation of our previous reviews on the topic and brings a survey of more than 120 papers published approximately since the second quarter of 2016 till the first quarter of 2018. It is organized according to the particular stacking principles and includes chapters on concentration adjustment (Kohlrausch) stacking, on stacking techniques based on pH changes, on stacking in electrokinetic chromatography and on other stacking techniques. Where available, explicit information is given about the procedure, electrolyte(s) used, detector employed and sensitivity reached. Not reviewed are papers on transient isotachophoresis which are covered by another review in this issue.

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Section: Introductionmentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

2018

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“…Thus, often before the analysis, different methods for pre‐fractionation or enrichment of proteins of interest are applied. For that purpose, variety of approaches can be employed including precipitation, (ultra)centrifugation, (ultra)filtration, SPE , SPME , large volume sample stacking, field‐amplified sample stacking, field‐amplified (also called field‐enhanced) sample injection, dynamic pH junction , and transient‐ITP (t‐ITP) .…”

Section: Sample Preparationmentioning

confidence: 99%

Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (2015–mid 2018)

Štěpánová

Kašička

2018

J of Separation Science

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This review summarizes recent developments and applications of capillary and microchip electroseparation methods in proteomic and peptidomic analyses since the year 2015 to ca. mid 2018. Sample preparation procedures for the removal of interfering components or for pre‐fractionation and preconcentration of proteins and peptides of interest are discussed. The innovations in coupling of capillary or microchip electroseparation methods with different modes of mass spectrometry detection are covered. In addition, significant recent applications of capillary electromigration methods in both bottom‐up and top‐down proteomics as well as in determinations of post‐translational modifications of proteins are presented. Moreover, several examples of the utilization of capillary electromigration methods coupled with mass spectrometry detection for clinical proteomics and peptidomics are described.

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“…126–129 Due to this inferior concentration sensitivity, various preconcentration methods are employed before or during the electrophoretic separation for enrichment of proteins. The development of efficient preconcentration strategies is of absolute necessity especially when dealing with biological samples where target proteins are usually present in low concentration.…”

Section: Separation Modes and Conditionsmentioning

confidence: 99%

Recent advances in protein analysis by capillary and microchip electrophoresis

Dawod

Arvin

Kennedy

2017

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120

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This review article describes the significant recent advances in analysis of proteins by capillary and microchip electrophoresis during the period mid 2014 to early 2017. The review highlights the progressions, new methodologies, innovative instrumental modifications, and challenges for efficient protein analysis in human specimens, animal tissues, and plant samples. Protein analysis fields covered in this review include analysis of native, reduced, and denatured proteins in addition to Western blotting, protein therapeutics and proteomics.

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Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Cited by 88 publications

References 209 publications

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (2015–mid 2018)

Recent advances in protein analysis by capillary and microchip electrophoresis

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