Characterization of Wild Apricot (Prunus armeniaca L.) Genotypes Selected from Cappadocia Region (Nevşehir-Turkey) by SSR Markers

Abstract

Cappadocia region of Anatolia hosts the third largest wild apricot population in Turkey. The objective of the study was to characterize 44 wild apricot genotypes selected from Cappadocia Region (Nevşehir-Turkey) as prominent with their late flowering, resistance to spring late frosts, large fruit sizes and/or late fruit ripening characteristics and 5 reference apricot cultivars ('Hacıhaliloğlu', 'Kabaaşı', 'Hasanbey', 'Aprikoz' and 'Levent') with SSR (simple sequence repeats) markers. A total of 16 SSR primers were used and 13 of them were successfully amplified. Total number of alleles was 107, average number of alleles was 8.23; average He and Ho values were 0.722 and 0.669, respectively. Polymorphism information content (PIC) values varied between 0.471 and 0.845. There was a quite high genetic diversity among wild apricot genotypes that genetic similarity values varied between 12 and 96%. Homonymous and synonymous genotypes were not encountered.

Utilizing SSR markers to examine the population structure and molecular genetic diversity of walnut (Juglans regia L.) genotypes in the Northwestern Himalayan region of Jammu and Kashmir

“…The WGAs with the highest PIC values were WGA-69, WGA-1, WGA-118, and WGA-276. At 0.22 per locus, WGA-202 exhibited the lowest PIC value, falling short of the 0.690 value reported by Bakir et al (2019) for Prunus armeniaca (wild form) when 16 microsatellite primers were utilized and the 0.546 value reported by Bourguiba et al (2012) when 24 SSR microsatellite primers were applied to Prunus armeniaca. The mean PIC value, as reported by Shah et al (2018), was 0.168.…”

Molecular Genetic Diversity of Prunus armeniaca L. (Rosaceae) Genotypes by RAPD, ISSR-PCR, and Chloroplast DNA (cpDNA) trnL-F Sequences

“…Molecular systematic information provides deeper insight into genetic structure in addition to analysis of genetic diversity through the classification of varieties (Kiani and Siahchehreh, 2017). In previous studies, genetic diversity of Prunus species has been analyzed using cpDNA (atpB-rbcL, trnL-trnF and rps16-trnQ) region (Batnini et al, 2019), internal transcribed spacer (ITS) region (Gilani et al, 2010), cpDNA trnL-F region (Gilani et al, 2011), ISSR (Li et al, 2013) and RAPD (Casas et al, 1999), SSR technique (Bakır et al, 2019).…”

“…In the ISSR analysis, 'Hacıhaliloğlu,' 'Çataloğlu,' and 'Kabaaşı' were found in the same group while the 'Hasanbey' genotype was found in a different group. Bakır et al (2019) selected 44 genotypes in Kapadokya Nevsehir (Turkey) having superior characteristics such as: Flowering later than wild apricots, resistant to late spring frosts, having large fruits, and delayed fruit ripening. Genetic relationship between the selected wild apricot genotypes were investigated with 13…”

Employing Barcode High-Resolution Melting Technique for Authentication of Apricot Cultivars

“…Combining the discrimination success of DNA barcoding and the easiness of HRM results in successful identification of food products such as PDO cheese (Ganopoulos et al 2013), codfish species (Shi et al 2020), poisonous plants (Thongkhao et al 2020), medicinal plants (Li et al 2016;Sun et al 2017;Mishra et al 2018), and bean crops (Madesis et al 2012). In a recent study, 16 SSR markers from the literature were used to characterise the wild apricot genotypes from Nevşehir region (Turkey) (Bakır et al 2019).…”