Characterization of two novel γ-gliadin genes encoded by K genome of Crithopsis delileana and evolution analysis with those from Triticeae

Abstract

By acid polyacrylamide gel electrophoresis (A-PAGE) analysis, it was indicated that the electrophoresis mobility of gliadins from Crithopsis delileana (Schult) Roshev (2n=2x=14, KK) had obvious difference with those from common wheat in α, γ and ω region. Using homologous primers, two γ-gliadin genes (gli-Kr1 and gli-Kr2) were isolated from C. delileana, which had been deposited in the GenBank under accession numbers EU283818 and EU283821, respectively. Two γ -gliadin genes of C. delileana had the similar primary structures to the corresponding gene sequences from other wheat related species. The differences were mainly resulted from substitutions, insertions and deletions involving single amino acid residues or motifs of γ-gliadins. The repetitive domains of gli-Kr1 and gli-Kr2 from C. delileana are shorter than most of other sequences. By the alignment of γ-gliadin genes from A, B, D, A m , A u , S, S l , S sh , S s and S b genomes of Triticum and Aegilops, R genome of Secale (γ-secalin), Ee genome of Lophopyrum and K genome of Crithopsis in Triticeae, phylogenetic analysis indicated that two γ-gliadin genes of C. delileana could be clustered together with a γ-gliadin genefrom S sh genome of Aegilops by an interior paralleled branch. It was the first time that the γ-gliadin genes encoded by K genome of C. delileana were characterized. These could offer precious information for better understanding the qualities associated with gliadins, the response in coeliac disease and studying the evolutionary relationship of gliadins in Triticeae.

Isolation and Characterization of a Psathyrostachys huashanica Keng 6Ns Chromosome Addition in Common Wheat

“…intermedium ssp. trichophorum [24], and C. delieana [25]. We found that the disomic addition line 59-11 had a specific band from P. huashanica in the α-gliadin section based on gliadin analysis (Fig.…”

Isolation and phylogenetic analysis of novel γ-gliadin genes in genus Dasypyrum

“…The γ-gliadins have been considered to be the most ancient members of the wheat glutenin (Anderson et al, 2001). The molecular cloning and sequencing of these genes are helpful for better understanding the relationships of their structures and functions, and will provide vital information on gene evolution (Guo et al, 2010). In the Triticeae, γ-gliadin genes have been cloned from the genus Triticum (Anderson et al, 2001;Qi et al, 2009;Altenbach et al, 2010), Aegilops (Qi et al, 2009;Huang et al, 2010), Thinopyrum , and Crithopsis (Guo et al, 2010).…”