SummaryHexaploid wheat (Triticum aestivum, genomes AABBDD) originated by hybridization of tetraploid Triticum turgidum (genomes AABB) with Aegilops tauschii (genomes DD). Genetic relationships between A. tauschii and the wheat D genome are of central importance for the understanding of wheat origin and subsequent evolution.Genetic relationships among 477 A. tauschii and wheat accessions were studied with the A. tauschii 10K Infinium single nucleotide polymorphism (SNP) array.Aegilops tauschii consists of two lineages (designated 1 and 2) having little genetic contact. Each lineage consists of two closely related sublineages. A population within lineage 2 in the southwestern and southern Caspian appears to be the main source of the wheat D genome. Lineage 1 contributed as little as 0.8% of the wheat D genome. Triticum aestivum is subdivided into the western and Far Eastern populations. The Far Eastern population conserved the genetic make-up of the nascent T. aestivum more than the western population. In wheat, diversity is high in chromosomes 1D and 2D and it correlates in all wheat D-genome and A. tauschii chromosomes with recombination rates.Gene flow from A. tauschii was an important source of wheat genetic diversity and shaped its distribution along the D-genome chromosomes.
To accurately quantify gene expression using quantitative PCR amplification, it is vital that one or more ideal internal control genes are used to normalize the samples to be compared. Ideally, the expression level of those internal control genes should vary as little as possible between tissues, developmental stages and environmental conditions. In this study, 32 candidate genes for internal control were obtained from the analysis of nine independent experiments which included 333 Affymetrix GeneChip Wheat Genome arrays. Expression levels of the selected genes were then evaluated by quantitative real-time PCR with cDNA samples from different tissues, stages of development and environmental conditions. Finally, fifteen novel internal control genes were selected and their respective expression profiles were compared using NormFinder, geNorm, Pearson correlation coefficients and the twofold-change method. The novel internal control genes from this study were compared with thirteen traditional ones for their expression stability. It was observed that seven of the novel internal control genes were better than the traditional ones in expression stability under all the tested cDNA samples. Among the traditional internal control genes, the elongation factor 1-alpha exhibited strong expression stability, whereas the 18S rRNA, Alpha-tubulin, Actin and GAPDH genes had very poor expression stability in the range of wheat samples tested. Therefore, the use of the novel internal control genes for normalization should improve the accuracy and validity of gene expression analysis.
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