“…The high value in identity found (64%) between panulirin and PjTI1 supports our primary sequence elucidation results and strongly indicates the occurrence of genes encoding panulirin-like inhibitors in the Panulirus genus. Interestingly, both the signal peptide and the cleaving site predicted in PjTI1 are highly similar to those described in defensin-like peptides from P. japonicus (50) and P. argus (26).…”
Section: Discussionmentioning
confidence: 68%
“…We have described here for first time how panulirin, a novel peptidase inhibitor, is involved in the regulation of the proPO cascade in lobsters. The results of this study, together with the recent discovery of novel genes encoding defensin-like antimicrobial peptides in lobsters (26,50), could indicate that Panulirus can be an attractive genus for deepening understanding of the immune system in crustaceans.…”
Background:The melanization reaction is an essential immune response in arthropods that should be tightly regulated. Results: A novel competitive and tight-binding trypsin inhibitor, panulirin, that inhibits the melanization response to lipopolysaccharides was found in lobster. Conclusion: Panulirin regulates serine peptidases in the pathway toward the activation of the prophenoloxidase enzyme. Significance: Serine peptidase inhibitors play a key role in controlling the immune response in arthropods.
“…The high value in identity found (64%) between panulirin and PjTI1 supports our primary sequence elucidation results and strongly indicates the occurrence of genes encoding panulirin-like inhibitors in the Panulirus genus. Interestingly, both the signal peptide and the cleaving site predicted in PjTI1 are highly similar to those described in defensin-like peptides from P. japonicus (50) and P. argus (26).…”
Section: Discussionmentioning
confidence: 68%
“…We have described here for first time how panulirin, a novel peptidase inhibitor, is involved in the regulation of the proPO cascade in lobsters. The results of this study, together with the recent discovery of novel genes encoding defensin-like antimicrobial peptides in lobsters (26,50), could indicate that Panulirus can be an attractive genus for deepening understanding of the immune system in crustaceans.…”
Background:The melanization reaction is an essential immune response in arthropods that should be tightly regulated. Results: A novel competitive and tight-binding trypsin inhibitor, panulirin, that inhibits the melanization response to lipopolysaccharides was found in lobster. Conclusion: Panulirin regulates serine peptidases in the pathway toward the activation of the prophenoloxidase enzyme. Significance: Serine peptidase inhibitors play a key role in controlling the immune response in arthropods.
“…1) and proteins (Fig. 2) show that signal peptides are highly conserved and mature peptides are relatively less conserved, which is also the character of PJD1 and PJD2 [10]. Defensins from different species are diverse.…”
Section: Similarities Domain Organization Multiple Alignment and Pmentioning
confidence: 95%
“…The similarity of the mature peptide of the six defensins is lower than that of the signal peptide. In the Japanese spiny lobster Panulirus japonicus, the signal peptides of two newly identified isoforms of defensin (designated PJD1 and PJD2) are in full agreement, whereas the mature peptides of PJD1 and PJD2 are diverse [10]. in HcDef1 and HcDef5 (the fourth and the eight cysteine) and in HcDef6 (the second and fifth cysteine) form an additional pair of disulfide bridges.…”
“…Many host AMPs have been shown to be induced or upregulated when the hosts are infected with pathogens (Pisuttharachai et al, 2009). For examples, transcripts of abaecin, although presence through all development stage in honeybee larvae, were found to be significantly upregulated 24 h following oral inoculation with the bacterial pathogen Paenibacillus larvae (Evans, 2004).…”
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