Characterization of the rax1 gene encoding a putative regulator of G protein signaling in Aspergillus fumigatus

Igbalajobi, Olumuyiwa; Yu, Jae‐Hyuk; Shin, Kwang‐Soo

doi:10.1016/j.bbrc.2017.04.079

Cited by 15 publications

(22 citation statements)

References 28 publications

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“…However, the function of its homologous proteins is poorly understood in filamentous fungi. Previously, we reported that Rax1 of A. fumigatus positively controls growth, development, and oxidative stress response [7]. To further understand the functions of Rax1, we carried out RNA-Seq-based genome-wide expression studies and additional functional investigation.…”

Section: Discussionmentioning

confidence: 99%

“…Glucose minimal medium (MMG) and MMG with 0.1% yeast extract (MMY) with appropriate supplements were used for culture of wild type (AF293) and the rax1 null mutant strains [7]. Conidia were propagated MMY for 5 days at 37 • C and were collected with 0.02% Tween 80.…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

“…In the genome of the opportunistic human pathogenic fungus Aspergillus fumigatus, six RGS proteins that contain the conserved RGS domain have been identified based on homology searches, and the roles of them have been characterized [5][6][7][8][9][10]. Previously, we reported that Rax1 of A. fumigatus positively controls vegetative growth and asexual development.…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we reported that Rax1 of A. fumigatus positively controls vegetative growth and asexual development. In addition, the rax1 null mutant conidia were more resistant against exogenous H 2 O 2 via elevated accumulation of intracellular trehalose and cell wall melanin [7]. In the present study, to further understand the functions of Rax1, we carried out RNA-Seq-based transcriptome analyses of wild types (WT) and rax1 null mutants.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic and Functional Studies of the RGS Protein Rax1 in Aspergillus fumigatus

et al. 2019

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In the comparative transcriptomic studies of wild type (WT) and rax1 null mutant strains, we obtained an average of 22,222,727 reads of 101 bp per sample and found that 183 genes showed greater than 2.0-fold differential expression, where 92 and 91 genes were up-and down-regulated in Δrax1 compared to WT, respectively. In accordance with the significantly reduced levels of gliM and casB transcripts in the absence of rax1, the Δrax1 mutant exhibited increased sensitivity to exogenous gliotoxin (GT) without affecting levels of GT production. Moreover, Δrax1 resulted in significantly restricted colony growth and reduced viability under endoplasmic reticulum stress condition. In summary, Rax1 positively affects expression of gliM and metacaspase genes.

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Section: Discussionmentioning

confidence: 99%

Section: Strains and Culture Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptomic and Functional Studies of the RGS Protein Rax1 in Aspergillus fumigatus

et al. 2019

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“…It has been reported that the putative hybrid GPCR-RGS protein GprK also plays an important role in upstream regulation of G-protein signaling and contributes to proper asexual sporulation, gliotoxin (GT) production, and oxidative stress responses (Jung et al, 2016 ). Recently, Rax1 was shown to positively control vegetative growth and asexual development, and modulate trehalose amount and cell wall melanin levels in conidia, and conidia resistance against hydrogen peroxide (Igbalajobi et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Characteristics of a Regulator of G-Protein Signaling (RGS) rgsC in Aspergillus fumigatus

Kim

Heo

et al. 2017

Front. Microbiol.

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The regulator of G-protein signaling (RGS) proteins have a conserved RGS domain that facilitates the intrinsic GTPase activity of an activated Gα subunit of heterotrimeric G protein, thereby attenuating signal transduction. Among six predicted RGS proteins in the opportunistic human pathogenic fungus Aspergillus fumigatus, only three (FlbA, GprK, and Rax1) have been studied. The unexplored RgsC composed of the Phox-associated (PXA), RGS, Phox homology (PX), and Nexin_C superfamily domains is highly conserved in many ascomycete fungi, suggesting a crucial role of RgsC in fungal biology. To address this, we have investigated functions of the rgsC gene. The deletion (Δ) of rgsC causes impaired vegetative growth and asexual development coupled with reduced expression of key developmental regulators. Moreover, ΔrgsC results in accelerated and elevated conidial germination regardless of the presence or absence of an external carbon source. Furthermore, ΔrgsC causes reduced conidial tolerance to oxidative stress. In addition, activities and expression of catalases and superoxide dismutases (SODs) are severely decreased in the ΔrgsC mutant. The deletion of rgsC results in a slight reduction in conidial tolerance to cell wall damaging agents, yet significantly lowered mRNA levels of cell wall integrity/biogenesis transcription factors, indicating that RgsC may function in proper activation of cell wall stress response. The ΔrgsC mutant exhibits defective gliotoxin (GT) production and decreased virulence in the wax moth larvae, Galleria mellonella. Transcriptomic studies reveal that a majority of transporters is down-regulated by ΔrgsC and growth of the ΔrgsC mutant is reduced on inorganic and simple nitrogen medium, suggesting that RgsC may function in external nitrogen source sensing and/or transport. In summary, RgsC is necessary for proper growth, development, stress response, GT production, and external nutrients sensing.

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Oxidative stress response pathways in fungi

Yaakoub

Mina

Calenda

et al. 2022

Cell. Mol. Life Sci.

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Characterization of the rax1 gene encoding a putative regulator of G protein signaling in Aspergillus fumigatus

Cited by 15 publications

References 28 publications

Transcriptomic and Functional Studies of the RGS Protein Rax1 in Aspergillus fumigatus

Transcriptomic and Functional Studies of the RGS Protein Rax1 in Aspergillus fumigatus

Characteristics of a Regulator of G-Protein Signaling (RGS) rgsC in Aspergillus fumigatus

Oxidative stress response pathways in fungi

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