Transcriptomic and Functional Studies of the RGS Protein Rax1 in Aspergillus fumigatus

Choi, Youngjoon; Lee, Minwoo; Igbalajobi, Olumuyiwa; Yu, Jae‐Hyuk; Shin, Kwang‐Soo

doi:10.3390/pathogens9010036

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…RgsB is also essential for endoplasmic reticulum stress and exogenous gliotoxin response. However, RgsB does not affect virulence in the murine model [41].…”

Section: Rgsb (Rax1)mentioning

confidence: 91%

Heterotrimeric G-Protein Signalers and RGSs in Aspergillus fumigatus

Park

Kim

et al. 2020

Pathogens

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The heterotrimeric G-protein (G-protein) signaling pathway is one of the most important signaling pathways that transmit external signals into the inside of the cell, triggering appropriate biological responses. The external signals are sensed by various G-protein-coupled receptors (GPCRs) and transmitted into G-proteins consisting of the α, β, and γ subunits. Regulators of G-protein signaling (RGSs) are the key controllers of G-protein signaling pathways. GPCRs, G-proteins, and RGSs are the primary upstream components of the G-protein signaling pathway, and they are highly conserved in most filamentous fungi, playing diverse roles in biological processes. Recent studies characterized the G-protein signaling components in the opportunistic pathogenic fungus Aspergillus fumigatus. In this review, we have summarized the characteristics and functions of GPCRs, G-proteins, and RGSs, and their regulatory roles in governing fungal growth, asexual development, germination, stress tolerance, and virulence in A. fumigatus.

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“…RgsB is also essential for endoplasmic reticulum stress and exogenous gliotoxin response. However, RgsB does not affect virulence in the murine model [41].…”

Section: Rgsb (Rax1)mentioning

confidence: 91%

Heterotrimeric G-Protein Signalers and RGSs in Aspergillus fumigatus

Park

Kim

et al. 2020

Pathogens

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“…We obtained raw fastq reads from 17 previously published and one newly generated bulk RNA-seq datasets (Table S1) (7,15,(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34). The adapter sequences were clipped with Trimmomatic (version 0.32, settings 2:30:10:2:keepBothReads LEADING:5 TRAILING:5 MINLEN:36) (35).…”

Section: Data Acquisition and Preprocessingmentioning

confidence: 99%

A network-based model ofAspergillus fumigatuselucidates regulators of development and defensive natural products of an opportunistic pathogen

Carriel,

Pyne,

Halberg-Spencer

et al. 2023

Preprint

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Aspergillus fumigatusis a notorious pathogenic fungus responsible for various harmful, sometimes lethal, diseases known as aspergilloses. Understanding the gene regulatory networks that specify the expression programs underlying this fungus’ diverse phenotypes can shed mechanistic insight into its growth, development, and determinants of pathogenicity. We used eighteen RNA-seq datasets (seventeen publicly available and one previously unpublished) ofAspergillus fumigatusto construct a comprehensive gene regulatory network resource. Our resource, named GRAsp (GeneRegulation ofAspergillus fumigatus), was able to recapitulate known regulatory pathways such as response to hypoxia, iron and zinc homeostasis, and secondary metabolite synthesis. Further, GRAsp was experimentally validated in two cases: one in which GRAsp accurately identified an uncharacterized transcription factor negatively regulating the production of the virulence factor gliotoxin and another where GRAsp revealed the bZip protein, AtfA, as required for fungal responses to microbial signals known as lipo-chitooligosaccharides. Our work showcases the strength of using network-based approaches to generate new hypotheses about regulatory relationships inAspergillus fumigatus. We also unveil an online, user-friendly version of GRAsp available to theAspergillusresearch community.

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Phylogeny of Regulators of G-Protein Signaling Genes in Leptographium qinlingensis and Expression Levels of Three RGSs in Response to Different Terpenoids

Gan

Tang

et al. 2022

Microorganisms

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Leptographium qinlingensis is a bark beetle-vectored pine pathogen in the Chinese white pine beetle (Dendroctonus armandi) epidemic in Northwest China. L. qinlingensis colonizes pines despite the trees’ massive oleoresin terpenoid defenses. Regulators of G-protein signaling (RGS) proteins modulate heterotrimeric G-protein signaling negatively and play multiple roles in the growth, asexual development, and pathogenicity of fungi. In this study, we have identified three L. qinlingensis RGS genes, and the phylogenetic analysis shows the highest homology with the regulators of G-protein signaling proteins sequence from Ophiostoma piceae and Grosmannia clavigera. The expression profiles of three RGSs in the mycelium of L. qinlingensis treated with six different terpenoids were detected, as well as their growth rates. Under six terpenoid treatments, the growth and reproduction in L. qinlingensis were significantly inhibited, and the growth inflection day was delayed from 8 days to 12–13 days. By analyzing the expression level of three RGS genes of L. qinlingensis with different treatments, results indicate that LqFlbA plays a crucial role in controlling fungal growth, and both LqRax1 and LqRgsA are involved in overcoming the host chemical resistances and successful colonization.

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Transcriptomic and Functional Studies of the RGS Protein Rax1 in Aspergillus fumigatus

Cited by 3 publications

References 24 publications

Heterotrimeric G-Protein Signalers and RGSs in Aspergillus fumigatus

Heterotrimeric G-Protein Signalers and RGSs in Aspergillus fumigatus

A network-based model ofAspergillus fumigatuselucidates regulators of development and defensive natural products of an opportunistic pathogen

Phylogeny of Regulators of G-Protein Signaling Genes in Leptographium qinlingensis and Expression Levels of Three RGSs in Response to Different Terpenoids

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