Characterization of Summer Savory (Satureja hortensis L.) Honey by Physico-Chemical Parameters and Chromatographic / Spectroscopic Techniques (GC-FID/MS, HPLC-DAD, UV/VIS and FTIR-ATR)

Jerković, Igor; Tuberoso, Carlo Ignazio Giovanni; Baranović, Goran; Marijanović, Zvonimir; Kranjac, Marina; Svečnjak, Lidija; Kuś, Piotr Marek

doi:10.5562/cca2514

Cited by 15 publications

(19 citation statements)

References 14 publications

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“…To our knowledge, no reports exist about the colorimetric analysis of pollen, and only a few papers dealt about the colorimetric evaluation of honey [ 59 , 60 ]. Moreover, literature data only reported colorimetric parameters for solved samples analysed by spectrophotometry, and did not show any reflectance curves.…”

Section: Resultsmentioning

confidence: 99%

Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS

et al. 2018

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Prostatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents. The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation. Pollen extracts have been previously reported as promising agents in managing clinical symptoms related to prostatitis. The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo. In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E2 (PGE2), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli. We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS). We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE2 levels, in rat prostate specimens. According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.

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Section: Resultsmentioning

confidence: 99%

Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS

et al. 2018

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“…In this figure, several distinct absorption peaks can be seen in the3405.88, 2427. 61 [15,25] and N-H group of amide A [15,26]. The peak at 2427.61 cm À1 was related to the C-H group of Alkanes [15,25,26].…”

Section: Fourier Transform Infrared (Ft-ir) Spectroscopymentioning

confidence: 98%

“…The purpose of the FT-IR experiment is the recognition of the possible functional groups of bio-molecules that are involved in capping, effective stabilization, and reduction of recently synthesized AgNPs [15,25]. FT-IR analysis of AgNPs was shown in Table 1 and Figure 4.…”

Section: Fourier Transform Infrared (Ft-ir) Spectroscopymentioning

confidence: 99%

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Anti-bacterial/fungal and anti-cancer performance of green synthesized Ag nanoparticles using summer savory extract

Ahmadi

Fazilati

Mousavi

et al. 2020

Journal of Experimental Nanoscience

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Nowadays, the expansion of nanoparticles as antimicrobial and anticancer agents is one of the most exciting methods for cancer therapy and microbial diseases that can vanquish the problems of drug resistance. In this research, the biosynthesis of silver nanoparticles (AgNPs) was stated using Summer Savory extract. Moreover, these AgNPs by UV-Vis spectroscopy, FTIR, XRD, FESEM, EDX, PSA, and zeta potential analysis were characterized, and by antimicrobial and anti-cancerous performance was evaluated. The maximum absorption peak at 472 nm by UV-Visible spectra confirmed the formation of Ag nanoparticles. The results obtained by FT-IR determined the presence of organic compounds such as amines, alkenes, alcohols, phenols, which performed significant roles in the fixating of these nanoparticles. The results obtained from FESEM, EDX, PSA, and Zeta Potential Analysis revealed that synthesized AgNPs had a hexagonal structure with a size ranging from 10 to 100 nm. The antimicrobial properties of the synthesized AgNPs were examined by Minimum Inhibitory Concentration, Minimum Bactericidal Concentration, and Minimum Fungal Concentration against five microbial strains included Klebsiella pneumonia, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, and Saccharomyces cerevisiae. The results demonstrated that biosynthesized AgNPs had the most antimicrobial activity, especially against E. Coli. The anti-cancerous results of these nanoparticles through MTT assay demonstrated exceptional anticancer effects against K-562 and MCF-7 human cancer cell lines with IC50 values 50 and 200 mg/ml, respectively.

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“…Gas chromatography-mass spectrometry (GC-MS) analysis. This analysis was performed according to a previously reported method 33 using an Agilent Technologies GC equipped with a mass selective detector, HP-5MS. A 5% phenyl methyl siloxane capillary column with dimensions of 30.0 m × 250 µm × 0.25 µm was used, and helium was used as the carrier gas at 1 ml/min.…”

Section: Effect Of Nacl Concentration Ph and Temperature On The Growthmentioning

confidence: 99%

In vitro dual (anticancer and antiviral) activity of the carotenoids produced by haloalkaliphilic archaeon Natrialba sp. M6

Hegazy

Abu‐Serie

Abo-Elela

et al. 2020

Sci Rep

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Halophilic archaea are a promising natural source of carotenoids. However, little information is available about the biological impacts of these archaeal metabolites. Here, carotenoids of Natrialba sp. M6, which was isolated from Wadi El-Natrun, were produced, purified and identified by Raman spectroscopy, Gc-mass spectrometry, and fourier transform infrared spectroscopy, Lc-mass spectrometry and nuclear magnetic resonance spectroscopy. the c 50 carotenoid bacterioruberin was found to be the predominant compound. Because cancer and viral hepatitis are serious diseases, the anticancer, anti-HcV and anti-HBV potentials of these extracted carotenoids (pigments) were examined for the first time. In vitro results indicated that the caspase-mediated apoptotic anticancer effect of this pigment and its inhibitory efficacy against matrix metalloprotease 9 were significantly higher than those of 5-fluorouracil. Furthermore, the extracted pigment exhibited significantly stronger activity for eliminating HcV and HBV in infected human blood mononuclear cells than currently used drugs. this antiviral activity may be attributed to its inhibitory potential against HcV RnA and HBV DnA polymerases, which thereby suppresses HcV and HBV replication, as indicated by a high viral clearance % in the treated cells. These novel findings suggest that the C 50 carotenoid of Natrialba sp. M6 can be used as an alternative source of natural metabolites that confer potent anticancer and antiviral activities. Halophilic archaea (haloarchaea) belong to the family Halobacteriaceae. This family includes a group of microorganisms that are able to live in hypersaline environments with high salt concentrations (up to 4 M), such as solar salterns, salt lakes and salt deposits 1. Interestingly, these organisms have received increasing attention due to their ability to produce a plethora of compounds with potential applications in many fields of biotechnology, including salt-tolerant enzymes, biodegradable polyesters, exopolysaccharides, antimicrobial halocins, biosurfactants, and photon-driven retinal protein 2. Most haloarchaeal species (e.g., Natrialba) can produce pigments, including carotenoids. Natrialba is an organism in our research that belongs to the Halobacteriaceae family. However, the genus has recently been reassigned to the novel family Natrialbaceae 3. Natrialba sp. M6 is an extreme haloalkaliphile that grows at pH 10.0 and 20-25% w/v NaCl and utilizes a wide range of carbohydrate and noncarbohydrate substrates. Carotenoids are lipid-soluble pigments that vary in colour between yellow, orange, and red. Carotenoids are classified based on the number of carbons in their backbones into the categories C 30 , C 40 and C 50. Most carotenoids exist as a C 40 structure in different living organisms, including bacteria, archaea, fungi, algae, and plants 4. Meanwhile, haloarchaea can produce C 50 bacterioruberin (BR), a rare carotenoid form that contains four hydroxyl groups 5. These pigments are divided into two major groups: xanthophylls (molecu...

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Characterization of Summer Savory (Satureja hortensis L.) Honey by Physico-Chemical Parameters and Chromatographic / Spectroscopic Techniques (GC-FID/MS, HPLC-DAD, UV/VIS and FTIR-ATR)

Cited by 15 publications

References 14 publications

Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS

Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS

Anti-bacterial/fungal and anti-cancer performance of green synthesized Ag nanoparticles using summer savory extract

In vitro dual (anticancer and antiviral) activity of the carotenoids produced by haloalkaliphilic archaeon Natrialba sp. M6

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