Characterization of some groups of gram-negative nonfermentative bacteria by the carbon source alkalinization technique

Abstract

Pseudomonas vesicularis CDC group WO-1 Alcaligenes faecalis I Alcaligenes faecalis II Alcaligenes odorans Alcaligenes denitrificans CDC group IVc-2 CDC group IVe Bordetella bronchiseptica Flavobacterium odoratum CDC group M-5 CDC group M-6 Acinetobacter calcoaceticus subsp Iwoffi Moraxella urethralis Moraxella osloensis Moraxella nonliquefaciens Moraxella phenylpyruvica Moraxella atlantae No. of strains 39

Identification of oxidase-positive, glucose-negative, motile species of nonfermentative bacilli

“…Upon initiating a search for additional tests to identify the oxidase-positive and glucose-negative species of nonfermentative bacilli, we elected to examine alkalinogenic substrates in ALP basal medium. Other investigators have used alkalinization of Simmons basal medium (15,17) or growth with other basal media (5,21) as an index of utilization of such substrates. However, procedures for detecting growth with mineral base media may not be readily applicable in a clinical laboratory, and we found that a significant minority of strains did not grow on Simmons basal medium with acetate, lactate, or pyruvate as substrate (unpublished data).…”

Ureide metabolism in plant-associated bacteria: purine plant-bacteria interactive scenarios under nitrogen deficiency

“…Xanthine Hypoxanthine Uric acid Allantoin References Actinopolyspora erythraea + (Duangmal et al 2016) A. xinjiangensis + (Duangmal et al 2016) Alcaligenes denitrificans − (Martin et al 1981) Aerobacter aerogenes + (Rouf 1968) Aerobacter fecalis − (Martin et al 1981) Amycolatopsis eurytherma, A. thermoflava + (Busarakam et al 2016) Angustibacter Paraburkholderia tropica + (Silva et al 2017b) Pedobacter sandarakinus − − (Yoon et al 2006) Peptococcus prevotii + (Reece et al 1976) Phenylobacterium composti − − (Weon et al 2008) Pseudomonas acidovorans + (Martin et al 1981) Ps. alcaligenes + (Martin et al 1981) Ps. aeruginosa + (Rouf 1968) Ps.…”

“…aeruginosa + (Rouf 1968) Ps. diminuta − (Martin et al 1981) Ps. mesoacidophila + (Loveridge et al 2017) Ps.…”

“…mesoacidophila + (Loveridge et al 2017) Ps. pseudoalcaligenes − (Martin et al 1981) Ps. putida + + + + (Fernández et al 2016) Ps.…”

“…putrefaciens + (Martin et al 1981) Ps. testosteroni + (Martin et al 1981) Ruania albidiflava − − (Gu et al 2007) Salmonella As mentioned previously, most of the PAB studied thus far harbor the complete set of genes for the purine metabolism as well as (i) the functional high-affinity transporters for adenine (PurP and YicO) or hypoxanthine/guanine (YjcD and YgfQ), belonging to cluster COG2252 of the evolutionarily broad family NCS2 in E. coli (Papakostas et al 2013), (ii) the NAT/ NCS2 (nucleobase ascorbate transporters or nucleobase cation symporter family 2), (iii) the NCS1 (nucleobase cation symporter family 1) purine transporters (Ma et al 2016), and (iv) the xanthine permease XanQ in E. coli K-12 (Frillingos 2012). Thus, proliferation of PAB with the ability to scavenge xanthine or hypoxanthine from the surrounding media could deplete the plant cells of these substrates for the enzymes XHD and XO.…”