Identification of oxidase-positive, glucose-negative, motile species of nonfermentative bacilli

Abstract

Motile and glucose-negative nonfermentative bacilli are difficult to identify by commonly used procedures. We found eight features (reduction of nitrate and nitrite, acidification of fructose, and alkalinization of acetamide, arginine, histidine, saccharate, and urea) that identified 83% of 143 strains. These, along with additional biochemical tests, effected identification of 93% of the strains. Hence, only a minority of these strains required examination by microscopy (for type of flagellation and presence of inclusion bodies) for complete identification.

Heterotrophic plate counts and the isolation of bacteria from mineral waters on selective and enrichment media

“…Others, such as CDC group IVc-2, have not so far been reported in bottled mineral waters. These strains are similar to strains of Bordetella bronchiseptica and share several diagnostic biochemical characteristics, but appear to be unrelated (Oberhofer 1985;Pickett & Greenwood 1986;Weaver et al 1983).…”

Recurrent CDC group IVc-2 bacteremia in a human with AIDS

“…They were negative for nitrate and nitrite reduction, indole production, gelatin and esculin hydrolysis, and oxidation-fermentation of glucose, fructose, lactose, maltose, mannitol, and xylose. Biochemically, the organism closely resembles Bordetella bronchiseptica and Oligella ureolytica (formerly CDC group IV e), from which it must be differentiated (11,13,14) (Table 2). Nitrate reduction and nitrite reduction are important in this differentiation in that the former two organisms reduce nitrate to nitrite, with Oligella ureolytica also reducing nitrite to N 2 .…”

Identification of clinical isolates of non-Enterobacteriaceae gram-negative rods by computer-assisted gas-liquid chromatography

“…By conventional methods, correct species identification of many oxidase-positive and -negative gram-negative rods, excluding members of the family Enterobacteriaceae, is frequently difficult and time-consuming (10,11). Gas chromatographic analysis of bacterial fatty acid methyl esters has provided a reasonably accurate, rapid, and cost-effective alternative for identification of isolates of many aerobic gram-positive and gram-negative microorganisms (1, 2, 4-7, 10, 15, 16).…”

“…), as well as the oxidase result and Gram stain morphology and reaction, were determined for each isolate. Depending on those results, a battery of additional tests was set up for each isolate from the following list: catalase, triple sugar iron agar, SIM's agar, tryptic soy broth cultures (to determine growth at 35 and 42ЊC and in the presence of 6.5% NaCl, as well as motility at 35ЊC), growth around X, V, and XV strips on brain heart infusion agar, relative growth on chocolate agar (aerobic and in 5 to 10% CO 2 ), lysine and ornithine decarboxylase (Moeller formulation), arginine dihydrolase (Moeller), urea, citrate, reduction of nitrate and nitrite, cetrimide agar (for growth, pigment, and fluorescence), acid production from O-F media (glucose, maltose, sucrose, lactose, xylose, and mannitol), DNase, egg yolk agar (for lecithinase and lipase), hydrolysis of casein, esculin, gel, and starch, methyl red-Voges-Proskauer, phenylalanine deaminase, and o-nitrophenyl-␤-D-galactopyranoside (3,8,11,12). Past antimicrobial agent responses for many of the species, as previously described by Gilardi (3), were also of assistance in determination of the identities of many isolates when compared with the responses determined in house for those isolates (9).…”