Pseudomonas alcaligenes andPseudomonas testosteroni: Characterization and identification

Pickett, M. J.; Greenwood, J.

doi:10.1007/bf01568946

Cited by 8 publications

(3 citation statements)

References 6 publications

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“…alcaligenes strains studied by Ralston-Barrett et al (24) were found to be 76 and 56% related to the type strain. This difference confirmed the heterogeneity shown by the phenotypic results of these authors (21,22).…”

Section: Rat-ttr Anaerobic Respiration Of Tetrathionate-tetrathionatsupporting

confidence: 87%

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Numerical Taxonomy of Pseudomonas alcaligenes, P. pseudoalcaligenes, P. mendocina, P. stutzeri, and Related Bacteria

Gavini

Holmes²,

Izard

et al. 1989

International Journal of Systematic Bacteriology

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A numerical phenotypic analysis, in which the unweighted pair group average linkage method and Dice similarity coefficient were used, was performed on 155 strains received as Pseudomonas alcaligenes, Pseudomonas pseudoalcaligenes, Pseudomonas mendocina, or Pseudomonas stutzeri. These organisms are the clinically important nonfluorescent species belonging to ribosomal ribonucleic acid group I of Palleroni and co-workers. Six major clusters, which could be further divided into 20 subclusters, were formed. Most strains received as P. alcaligenes fell into three subclusters (subclusters Al, A2, and Bl), whereas strains received as P. pseudoalcaligenes were mainly classified in two other subclusters (subclusters C2 and C3). All but two strains (subcluster D1) of organisms received as P. mendocina were grouped in subcluster D2. Most of the 45 strains received as P. stutzeri were contained in a large subcluster, subcluster E2 (39 strains). Strains belonging to fluorescent pseudomonad species (Pseudomonas aeruginosa, Pseudomonas jluorescens, and Pseudomonas putida), which were included in the analysis for control purposes, were contained in one cluster, which comprised seven subclusters.During recent years, Pseudomonas spp. strains have been studied with increasing interest because of their importance in medical and food microbiology and in phytopathology .Since the classical study of Stanier et al. (30) on Pseudomonas taxonomy, most of the analyses performed on these strains have pointed to the genomic heterogeneity of the genus (5, 17, 35). However, very few studies have been carried out at the species level to revise the phenotypic definitions of these organisms and to determine their genomic positions by deoxyribonucleic acid (DNA)-DNA hybridization. Only fluorescent Pseudomonas spp. strains and strains isolated from meat have been the subjects of several taxonomic studies at the species level (2, 3, 14-16, 24, 26, 34). Although the nutritional characteristics given by Stanier et al. (30) are still potentially the most useful for differentiation, the lack of other phenotypic data leads to difficulties in developing identification schemes.The aim of this study was to define, by using a numerical phenotypic analysis, species known to belong to ribosomal ribonucleic acid group I (18) on the basis of DNA-ribosomal ribonucleic acid hybridization (17), particularly the clinically important species of this group, which were received as Pseudomonas alcaligenes, Pseudomonas pseudoalcaligenes, Pseudomonas mendocina, or Pseudomonas stutzeri. Later, we intend to study the phenotypic groups by using DNA-DNA hybridization. MATERIALS AND METHODSBacterial strains. A total of 155 strains were included in the analysis. These organisms comprised type and other reference strains received from diverse culture collections as P. alcaligenes, P. pseudoalcaligenes, P. mendocina, or P. stutzeri. Strains belonging to Pseudomonas f luorescens biovars A, B, C, F, and G, Pseudomonas chlororaphis, Pseu-* Corresponding author. domonas aureofacie...

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Section: Rat-ttr Anaerobic Respiration Of Tetrathionate-tetrathionatsupporting

confidence: 87%

“…alcaligenes from Pseudomonas testosteroni (21,22). The latter species and Pseudomonas acidovorans have been reclassified in the genus Comamonas (6) as Comamonas testosteroni and Comamonas acidovorans (33), respectively, and it is necessary to be able to separate them from P .…”

Section: Rat-ttr Anaerobic Respiration Of Tetrathionate-tetrathionatmentioning

confidence: 99%

Numerical Taxonomy of Pseudomonas alcaligenes, P. pseudoalcaligenes, P. mendocina, P. stutzeri, and Related Bacteria

Gavini

Holmes²,

Izard

et al. 1989

International Journal of Systematic Bacteriology

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“…Our chemotaxonomic results excluded alternative identification of isolates P-3, PSB 4, T 2, and A 3, and Comamonas testosteroni DSM 1622 as Pseudomonas alcaligenes strains, as suggested by the results of the multiple-test system (Table 1). Insufficient differentiation of the latter two species not only is a failure of the API 20NE multiple-test system, but also is a general problem of the present phenotypic approaches which has attracted much attention (23,49,50). However, as we demonstrated, analysis of either quinones or polyamines can easily solve this problem because P .…”

Section: Phenotypic Characterizationmentioning

confidence: 99%

Identification of Xenobiotic-Degrading Isolates from the Beta Subclass of the Proteobacteria by a Polyphasic Approach Including 16S rRNA Partial Sequencing

Busse

El-Banna

Oyaizu

et al. 1992

International Journal of Systematic Bacteriology

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Nineteen gram-negative, aerobic, biodegradative isolates were identified by using a polyphasic taxonomic approach. The presence of the specific polyamine 2-hydroxyputrescine and the presence of a ubiquinone with eight isoprenoid units in the side chain (ubiquinone Q-8) allowed allocation of these organisms to the beta subclass of the Proteobacteria. On the basis of the results of additional characterization experiments (i.e., API 20NE tests, determinations of soluble protein patterns, and DNA-DNA hybridization experiments), we classified six isolates as either Comamonas testosteroni, Comamonas acidovorans, or Alcaligenes xylosoxidans subsp. denitn3cans. By using the same criteria we allocated two additional isolates to the genus Alcaligenes. A comparison of a 16s rRNA fragment (positions 1220 to 1377; Escherichia coli nomenclature) indicated that the remaining isolates should be allocated as follows: one is a member of C. testosteroni and one is a member of Acidovorax facilis, as confirmed by the results of additional DNA-DNA hybridizations; two others probably belong to the family Alcaligenaceae; six are related to "Alcaligenes eutrophus"; and one, strain NRRL 12228, occupies an isolated position.

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Comamonas

Willems¹,

Vos²

2006

The Prokaryotes

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Pseudomonas alcaligenes andPseudomonas testosteroni: Characterization and identification

Cited by 8 publications

References 6 publications

Numerical Taxonomy of Pseudomonas alcaligenes, P. pseudoalcaligenes, P. mendocina, P. stutzeri, and Related Bacteria

Numerical Taxonomy of Pseudomonas alcaligenes, P. pseudoalcaligenes, P. mendocina, P. stutzeri, and Related Bacteria

Identification of Xenobiotic-Degrading Isolates from the Beta Subclass of the Proteobacteria by a Polyphasic Approach Including 16S rRNA Partial Sequencing

Comamonas

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