Characterization of soluble and exosomal forms of the EGFR released from pancreatic cancer cells

Glypican-1 identifies cancer exosomes and detects early pancreatic cancer

“…Melanoma-derived exosomes contain the tumor-associated antigen Mart-1 and tyrosinase-related protein-2 (TYRP2) 15–17 . Exosomes from gastric, breast and pancreas cancer carry members of the human epidermal growth factor receptor (HER) family 18–20 . None of these markers are however specific to cancer-derived exosomes.…”

Highly skewed distribution of miRNAs and proteins between colorectal cancer cells and their exosomes following Cetuximab treatment: biomolecular, genetic and translational implications

“…This latter phenomenon is biologically consistent with results reported by Ciravolo et al and Battke et al on Trastuzumab-treated breast carcinoma cells [33, 54]: exosomes secreted by HER2-overexpressing breast carcinoma cell lines express HER2 molecule, enabling them to bind to HER2 antibody Trastuzumab both in vitro and in vivo ; these exosomes efficiently bound and sequestered tumor-reactive antibodies and dramatically reduced their binding to tumor cells: in this way, they inhibited the overall effect of Trastuzumab on cancer cells proliferation. Given that exosomes express different forms of EGFR, the molecular target of Cetuximab [55 - 57], extracellular binding between Cetuximab and exosomal EGFR could reduce antibody binding to CRC cancer cells, partially neutralizing the effects of antibody-based therapeutics.…”

Establishment of a heterotypic 3D culture system to evaluate the interaction of TREG lymphocytes and NK cells with breast cancer

“…Furthermore, overlay of fluorescence data with light microscopy photomicrographs proved inadequate in distinguishing cell boundaries within the filamentous GFRM. Since the antibody employed recognises the 170 kDA EGFR isoform via western blot, it is expected that the staining in the extracellular matrix is not due to the soluble form of EGFR but rather may correspond to that sequestered in exosomes, implicated in immune suppression and cellular communication necessary for tumour progression, trapped within the GFRM (Adamczyk et al, 2011;Zhang and Grizzle, 2011).…”