Establishment of a heterotypic 3D culture system to evaluate the interaction of TREG lymphocytes and NK cells with breast cancer

Augustine, Tanya N.; Dix-Peek, Thérèse; Duarte, Raquel; Candy, Geoffrey P.

doi:10.1016/j.jim.2015.07.003

Cited by 30 publications

(35 citation statements)

References 93 publications

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“…Frequently, 3D culture models of neoplasms, including EOC, are achieved by culturing tissue fragments, suspension of primary cells or cell lines upon a natural or synthetic polymer matrix [23][24][25][26][27]34,35]. Another way to generate 3D cultures of malignant cells is based on the use of ULA containers that prevent cell adhesion, which combined with suitable culture media and/or agitation promote the growth of epithelial cells as free cell aggregates [36][37][38].…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, three-dimensional (3D) cell cultures provide in vitro models with improved tissue-like characteristics, placing them between the in vitro 2D models and the in vivo models [20][21][22]. 3D culture furnishes in vitro models to study the interaction between malignant cells and extracellular matrix, mechanisms of malignant invasion, susceptibility and resistance of malignant cells to drugs [23][24][25][26][27]. Recently, standardized high-throughput 3D culture systems have become commercially available, raising questions on whether they would be useful for studies on the interactions of immune cells in the tumor microenvironment.…”

Section: Introductionmentioning

confidence: 99%

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Three-Dimensional Cell Culture Based on Magnetic Fields to Assemble Low-Grade Ovarian Carcinoma Cell Aggregates Containing Lymphocytes

Souza-Araújo

Tonetti

Cardoso

et al. 2020

Cells

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There is a limited number of established ovarian cancer cell lines matching the low-grade serous histotype available for research purposes. Three-dimensional (3D) culture systems provide in vitro models with better tissue-like characteristics than two-dimensional (2D) systems. The goal in the study was to characterize the growth of a given low-grade serous ovarian carcinoma cell line in a 3D culture system conducted in a magnetic field. Moreover, the culture system was evaluated in respect to the assembly of malignant cell aggregates containing lymphocytes. CAISMOV24 cell line alone or mixed with human peripheral blood mononuclear cells (PBMC) were cultured using a commercially available 3D culture system designed for 24 well plates. Resulting cell aggregates revealed the intrinsic capacity of CAISMOV24 cells to assemble structures morphologically defined as papillary, and reflected molecular characteristics usually found in ovarian carcinomas. The contents of lymphocytes into co-cultured cell aggregates were significantly higher (p < 0.05) when NanoShuttle-conjugated PBMC were employed compared with non-conjugated PBMC. Moreover, lymphocyte subsets NK, T-CD4, T-CD8 and T-regulatory were successfully retrieved from co-cultured cell aggregates at 72h. Thus, the culture system allowed CAISMOV24 cell line to develop papillary-like cell aggregates containing lymphocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Cell Culture Based on Magnetic Fields to Assemble Low-Grade Ovarian Carcinoma Cell Aggregates Containing Lymphocytes

Souza-Araújo

Tonetti

Cardoso

et al. 2020

Cells

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show abstract

“…In this study, NK cells were able to disrupt MCF-7 cell masses regardless of T REG presence; whereas under T REG presence alone the cell clusters remained unchanged. NK cells also disrupted MDA-MB-231 cell networks regardless of T REG presence, and in the co-culture with just T REG and cancer cells distinct stellate networks were noted [37].…”

Section: Lymphocytesmentioning

confidence: 94%

The next level of 3D tumour models: immunocompetence

Nyga

Neves²,

Stamati

et al. 2016

Drug Discovery Today

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The complexity of the tumour microenvironment encompasses interactions between cancer and stromal cells. Moving from 2D cell culture methods into 3D models enables more-accurate investigation of those interactions. Current 3D cancer models focus on cancer spheroid interaction with stromal cells, such as fibroblasts. However, over recent years, the cancer immune environment has been shown to have a major role in tumour progression. This review summarises the state-of-art on immunocompetent 3D cancer models that, in addition to cancer cells, also incorporate immune cells, including monocytes, cancer-associated macrophages, dendritic cells, neutrophils and lymphocytes.

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“…Additionally, 3D in vitro models overcome the drawbacks of murine models in terms of scalability, cost and ease of use. Isolated cancer cells co-cultured in 3D hydrogels or scaffolds represent a way to study the interactions with lymphocytes [31] in a condition more comparable to the in vivo microenvironment. Thus, 3D in vitro tumour models allowing more physiologically relevant insights into tumour mass and immune system interactions in the TME could represent a more valuable assay for improved preclinical tests for immunotherapies against solid tumours in combination with or as an alternative to conventional methods.…”

Section: Tumour Models For T Cell Immunotherapymentioning

confidence: 99%

Microfluidic models for adoptive cell-mediated cancer immunotherapies

Adriani

Pavesi

Tan

et al. 2016

Drug Discovery Today

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Current adoptive T cell therapies have shown promising results in clinical trials but need further development as an effective cancer treatment. Here, we discuss how 3D microfluidic tumour models mimicking the tumour microenvironment could help in testing T cell immunotherapies by assessing engineered T cells and identifying combinatorial therapy to improve therapeutic efficacy. We propose that 3D microfluidic systems can be used to screen different patient-specific treatments, thereby reducing the burden of in vivo testing and facilitating the rapid translation of successful T cell cancer immunotherapies to the clinic.

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Establishment of a heterotypic 3D culture system to evaluate the interaction of TREG lymphocytes and NK cells with breast cancer

Cited by 30 publications

References 93 publications

Three-Dimensional Cell Culture Based on Magnetic Fields to Assemble Low-Grade Ovarian Carcinoma Cell Aggregates Containing Lymphocytes

Three-Dimensional Cell Culture Based on Magnetic Fields to Assemble Low-Grade Ovarian Carcinoma Cell Aggregates Containing Lymphocytes

The next level of 3D tumour models: immunocompetence

Microfluidic models for adoptive cell-mediated cancer immunotherapies

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