Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Abstract

Objectives: This study aimed to investigate Salmonella Enteritidis strains isolated from human and food sources in the north of Morocco by means of phenotypic and genotypic methods. Methodology: Fifteen isolates from humans and food were submitted to phage typing, XbaI-macrorestriction (pulsed field gel electrophoresis [PFGE]), enterobacterial repetitive intergenic consensus (ERIC-PCR), antimicrobial susceptibility testing, and PCR assay targeting the spvR and invA genes. Results: Six fingerprinting profiles were obtained with the ERIC-PCR method, four with PFGE profiling, five with antimicrobial resistance, three with phage typing, and only one with plasmid profiling. spvR gene was detected in six strains, which did not harbour plasmids of 90 kb. Conclusions: The conclusions of this study are drawn from a limited number of isolates. It would be desirable to investigate a greater and more diverse population of Salmonella isolates. S. Enteritidis was genotyped and showed four different patterns by PFGE and six by ERIC-PCR. Accordingly, high genetic similarity and limited genetic diversity were found for these strains from north of Morocco.

Outbreak of multidrug resistant Salmonella Typhimurium in calves at a veterinary hospital in Brazil

“…To better understand the transmission patterns during the outbreak, one S. Typhimurium isolate from each animal (n=7) (Figure 2) were fingerprinted by ERIC-PCR (VERSALOVIC et al, 1991). This molecular tool have been shown to efficiently fingerprinted Salmonella strains in outbreak investigations with a high discriminatory power (AMMARI et al, 2009;SMITH et al, 2011;HASHEMI & BAGHBANI-ARANI, 2015;PURIGHALLA et al, 2017). In the present report, the majority of the isolates showed an identical pattern in the ERIC-PCR (Figure 2), suggesting all emerged from the same clone.…”

Comparison of four molecular methods to typeSalmonellaEnteritidis strains

“…Enteritidis strains, complementing the phenotypic assays. However, some studies have presented variable results related to the ability of specific techniques to differentiate the relatively genetically homogeneous serovar Enteritidis .…”

Aetiology, epidemiology and clinical characteristics of acute moderate-to-severe diarrhoea in children under 5 years of age hospitalized in a referral paediatric hospital in Rabat, Morocco

“…Local data about the presence of diarrhoeagenic bacteria in different food products can also be found (Bennani et al, 2011), and specific data regarding infections by Salmonella spp. have been published (Ammari et al, 2009). However, a comprehensive description of the epidemiology and aetiology of diarrhoea in Morocco remains to be performed.…”