Comparison of four molecular methods to type<i>Salmonella</i>Enteritidis strains

Campioni, Fábio; Pitondo-Silva, André; Bergamini, Alzira Maria Morato; Falcão, Juliana Pfrimer

doi:10.1111/apm.12367

Cited by 21 publications

(18 citation statements)

References 31 publications

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“…The Enteritidis isolates were highly homogeneous, displaying the same RAPD fingerprint pattern, all harbored pSV, and the three MLST sequenced isolates were ST11. This is considered the founder genotype for the Enteritidis population, and has been reported in other countries of South America [ 45 , 46 ]. Only one Enteritidis isolate was MDR, but no evidence of integrons was found, and 16 had ISC.…”

Section: Discussionmentioning

confidence: 84%

Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods

et al. 2017

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In this study, different molecular typing tools were applied to characterize 95 Salmonella enterica blood isolates collected between 2008 and 2013 from patients at nine public hospitals in Lima, Peru. Combined results of multiplex PCR serotyping, two- and seven-loci multilocus sequence typing (MLST) schemes, serotyping, IS200 amplification and RAPD fingerprints, showed that these infections were caused by eight different serovars: Enteritidis, Typhimurium, Typhi, Choleraesuis, Dublin, Paratyphi A, Paratyphi B and Infantis. Among these, Enteritidis, Typhimurium and Typhi were the most prevalent, representing 45, 36 and 11% of the isolates, respectively. Most isolates (74%) were not resistant to ten primarily used antimicrobial drugs; however, 37% of the strains showed intermediate susceptibility to ciprofloxacin (ISC). Antimicrobial resistance integrons were carried by one Dublin (dfra1 and aadA1) and two Infantis (aadA1) isolates. The two Infantis isolates were multidrug resistant and harbored a large megaplasmid. Amplification of spvC and spvRA regions showed that all Enteritidis (n = 42), Typhimurium (n = 34), Choleraesuis (n = 3) and Dublin (n = 1) isolates carried the Salmonella virulence plasmid (pSV). We conclude that the classic serotyping method can be substituted by the multiplex PCR and, when necessary, sequencing of only one or two loci of the MLST scheme is a valuable tool to confirm the results. The effectiveness and feasibility of different typing tools is discussed.

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Section: Discussionmentioning

confidence: 84%

Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods

et al. 2017

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“…Aberdeen isolates by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and clustered regularly interspaced short palindromic repeat (CRISPR) analysis [14–18], with the aim to clarify the molecular basis of the inability of these isolates to produce H 2 S.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Salmonella enterica Serovar Aberdeen Negative for H2S Production in China

Xie

et al. 2016

PLoS ONE

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Salmonella enterica infections continue to be a significant burden on public health worldwide. The ability of S. enterica to produce hydrogen sulfide (H2S) is an important phenotypic characteristic used to screen and identify Salmonella with selective medium; however, H2S-negative Salmonella have recently emerged. In this study, the H2S phenotype of Salmonella isolates was confirmed, and the selected isolates were subjected to antimicrobial susceptibility testing and molecular identification by multilocus sequence typing, pulsed-field gel electrophoresis, and clustered regularly interspaced short palindromic repeat (CRISPR) analysis. The phs genetic operon was also analyzed. A total of 160 S. enterica serovar Aberdeen isolates were detected between 2005 and 2013 in China. Of them, seven non-H2S-producing isolates were detected. Notably, four samples yielded four pairs of isolates with different H2S phenotypes, simultaneously. The data demonstrated that H2S-negative isolates were genetically closely related to H2S-positive isolates. Three new spacers (Abe1, Abe2, and Abe3) were identified in CRISPR locus 1 in four pairs of isolates with different H2S phenotypes from the same samples. Sequence analysis revealed a new nonsense mutation at position 208 in the phsA gene of all non-H2S-producing isolates. Additionally, we describe a new screening procedure to avoid H2S-negative Salmonella, which would normally be overlooked during laboratory and hospital screening. The prevalence of this pathogen may be underestimated; therefore, it is important to focus on improving surveillance of this organism to control its spread.

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“…These methods have been used in combination with well-established standard techniques, such as serotyping and phage typing in epidemiological studies, improving the differentiation of strains (Tavechio, 2006 ). In this review, we summarizes the molecular approaches that have been used in the currently ST surveillance networks and disease outbreak investigations as PFGE, MLVA, MLST, CRISPR, Ribotyping, and WGS (Liu W. et al, 2011 ; Shariat et al, 2013 ; Shariat and Dudley, 2014 ; Campioni et al, 2015 ; Almeida et al, 2017 ). Besides, we compared the results obtained with different researches to identify more effective and appropriate method or methods for ST tracing.…”

Section: Genotypic Methodsmentioning

confidence: 99%

Phenotypic and Genotypic Eligible Methods for Salmonella Typhimurium Source Tracking

2017

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Salmonellosis is one of the most common causes of foodborne infection and a leading cause of human gastroenteritis. Throughout the last decade, Salmonella enterica serotype Typhimurium (ST) has shown an increase report with the simultaneous emergence of multidrug-resistant isolates, as phage type DT104. Therefore, to successfully control this microorganism, it is important to attribute salmonellosis to the exact source. Studies of Salmonella source attribution have been performed to determine the main food/food-production animals involved, toward which, control efforts should be correctly directed. Hence, the election of a ST subtyping method depends on the particular problem that efforts must be directed, the resources and the data available. Generally, before choosing a molecular subtyping, phenotyping approaches such as serotyping, phage typing, and antimicrobial resistance profiling are implemented as a screening of an investigation, and the results are computed using frequency-matching models (i.e., Dutch, Hald and Asymmetric Island models). Actually, due to the advancement of molecular tools as PFGE, MLVA, MLST, CRISPR, and WGS more precise results have been obtained, but even with these technologies, there are still gaps to be elucidated. To address this issue, an important question needs to be answered: what are the currently suitable subtyping methods to source attribute ST. This review presents the most frequently applied subtyping methods used to characterize ST, analyses the major available microbial subtyping attribution models and ponders the use of conventional phenotyping methods, as well as, the most applied genotypic tools in the context of their potential applicability to investigates ST source tracking.

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Comparison of four molecular methods to typeSalmonellaEnteritidis strains

Cited by 21 publications

References 31 publications

Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods

Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods

Molecular Characterization of Salmonella enterica Serovar Aberdeen Negative for H2S Production in China

Phenotypic and Genotypic Eligible Methods for Salmonella Typhimurium Source Tracking

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