Characterization of rice yellow mottle virus isolatesin Sudano-Sahelian areas

Konaté, G.; Traoré, Oumar; Coulibaly, Mamadou

doi:10.1007/s007050050146

Cited by 65 publications

(44 citation statements)

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“…Inoculum was prepared by grinding infected leaf tissue with cold distilled water (Abo et al 2005) at the ratio of 1:10 (w/v). Inoculations were accomplished by dipping a piece of cheesecloth in the virus extract and then rubbing it on the upper surface of carborundum (600 mesh; 5 mg/ml) dusted leaves (Konaté et al 1997(Konaté et al , 2001). Excess inoculum was washed off with distilled water (Noordam 1973).…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, dissemination by contact between healthy and infected plants, fluid from diseased plants, RYMV-contaminated hands, and chrysomelid beetles has been demonstrated (Konaté et al 1997;Abo et al 2003a;Sarra & Peters 2003;Traoré et al 2006). The most important weed hosts include Echinochloa colona (L.) Link, Panicum repens L., and Ischaemum rugosum Salisb.…”

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Relative resistance to Rice yellow mottle virus in rice

Salaudeen¹

2014

Plant Prot. Sci.

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Salaudeen M.T. (2014): Relative resistance to Rice yellow mottle virus in rice. Plant Protect. Sci., 50: 1-7.We identified sources of Rice yellow mottle virus (RYMV) resistance in rice cultivars. Eight cultivars together with susceptible and resistant controls were evaluated under screenhouse conditions as inoculated and uninoculated treatment in completely randomised design with three replications. Seedlings were inoculated with the virus by sap transmission at two weeks after sowing. Disease incidence and severity (scale 1-9: 1-3 = green leaves with sparse dots or streaks, 9 = yellow or orange leaves and some plant dead), yield, and agronomic traits were recorded. Data analyses included Area Under the Disease Progress Curve (AUDPC), independent t-test, and Analysis of Variance. According to differences in most measured traits control cultivars FARO 29 and Gigante were proved to be the most susceptible and partially tolerant ones, respectively. Cvs FARO 12, FARO 17, FARO 37, and FARO 52 were classified as partially tolerant. Uninoculated control plants performed better than the inoculated for all the yield and agronomic parameters. Reduction in plant height (6%) and number of tillers per plant (4.8%), increased days to heading (3 days), and reduction in paddy yield (6.5%) was lowest in cv. Gigante. Paddy yield per plant of the RYMV-inoculated was the highest in cv. Gigante (2.4 g). The rice cultivars which combined RYMV-resistance with high-yield could be utilised in rice breeding programmes in order to enhance food security.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Relative resistance to Rice yellow mottle virus in rice

Salaudeen¹

2014

Plant Prot. Sci.

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“…RYMV variability was first apparent from the detection of several serotypes in immunological studies with polyclonal and monoclonal antibodies (13,20,23,29). Moreover, sequencing of the coat protein gene revealed genetic variation within each serotype (11).…”

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confidence: 99%

Inferring the Evolutionary History of Rice Yellow Mottle Virus from Genomic, Phylogenetic, and Phylogeographic Studies

Fargette

Pinel

Abubakar

et al. 2004

J Virol

106

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Fourteen isolates of Rice yellow mottle virus (RYMV) were selected as representative of the genetic variability of the virus in Africa from a total set of 320 isolates serologically typed or partially sequenced. The 14 isolates were fully sequenced and analyzed together with two previously reported sequences. RYMV had a genomic organization similar to that of Cocksfoot mottle sobemovirus. The average nucleotide diversity among the 16 isolates of RYMV was 7%, and the maximum diversity between any two isolates was 10%. A strong conservative selection was apparent on both synonymous and nonsynonymous substitutions, through the amino acid replacement pattern, on the genome size, and through the limited number of indel events. Furthermore, there was a lack of positive selection on single amino acid sites and no evidence of recombination events. RYMV diversity had a pronounced and characteristic geographic structure. The branching order of the clades correlated with the geographic origin of the isolates along an east-to-west transect across Africa, and there was a marked decrease in nucleotide diversity moving westward across the continent. The insertion-deletion polymorphism was related to virus phylogeny. There was a partial phylogenetic incongruence between the coat protein gene and the rest of the genome. Overall, our results support the hypothesis that RYMV originated in East Africa and then dispersed and differentiated gradually from the east to the west of the continent.

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“…Isolates representative of all the geographic zones where the disease is present have been collected, and their variability has been analyzed. RYMV diversity was first apparent from the detection of several serotypes in immunological studies with polyclonal and monoclonal antibodies (13,14,17,18). Subsequently, five major serotypes (named Sr1 to Sr5) were defined using a set of discriminant monoclonal antibodies.…”

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Internal Point Mutations of the Capsid Modify the Serotype of Rice Yellow Mottle Virus

Hébrard

Pinel‐Galzi

Catherinot

et al. 2005

J Virol

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Rice yellow mottle virus is classified in five major serotypes; the molecular diversity of the coat protein (CP) is well established, but the amino acids involved in the recognition by discriminant monoclonal antibodies (MAbs) remain unknown. Reconstruction of a phylogenetic tree and sequence alignment of the CP gene of a sample representative of the continental-large diversity were used to identify 10 serospecific amino acids (i.e., conserved in all isolates belonging to the same serotype and distinct in other serotypes). Positions occupied by serospecific residues were localized on the crystal structure of the CP monomer and on modeled capsomers. Structural, molecular, and serological properties of each serotype were analyzed, and subsequently, hypotheses on the potential role of amino acids in discriminating reactions with antibodies were formulated. The residues 114 and 115 (serospecific of Sr1) and 190 (serospecific of Sr2) were localized on the outer surface of the capsid and might be directly involved in the immunoreactivity with MAb D and MAb A, respectively. In contrast, residues 180 (Sr3) and 178 (Sr5) lay within the inner surface of the capsid. To understand the role of these internal positions in the recognition with the antibodies, two substitutions (T180K and G178D) were introduced in the CP of an infectious clone. These mutations modified the antigenicity with MAb G and MAb E discriminating Sr3 and Sr5, respectively, while the reaction with MAb D remained unaffected. This result suggests an indirect effect of these two internal mutations on local immunostructure while the global structure was maintained.

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Characterization of rice yellow mottle virus isolatesin Sudano-Sahelian areas

Cited by 65 publications

References 2 publications

Relative resistance to Rice yellow mottle virus in rice

Relative resistance to Rice yellow mottle virus in rice

Inferring the Evolutionary History of Rice Yellow Mottle Virus from Genomic, Phylogenetic, and Phylogeographic Studies

Internal Point Mutations of the Capsid Modify the Serotype of Rice Yellow Mottle Virus

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