IntroductionIn the era of malaria elimination and eradication, drug-based and vaccine-based approaches to reduce malaria transmission are receiving greater attention. Such interventions require assays that reliably measure the transmission of Plasmodium from humans to Anopheles mosquitoes.MethodsWe compared two commonly used mosquito feeding assay procedures: direct skin feeding assays and membrane feeding assays. Three conditions under which membrane feeding assays are performed were examined: assays with i) whole blood, ii) blood pellets resuspended with autologous plasma of the gametocyte carrier, and iii) blood pellets resuspended with heterologous control serum.Results930 transmission experiments from Cameroon, The Gambia, Mali and Senegal were included in the analyses. Direct skin feeding assays resulted in higher mosquito infection rates compared to membrane feeding assays (odds ratio 2.39, 95% confidence interval 1.94–2.95) with evident heterogeneity between studies. Mosquito infection rates in membrane feeding assays and direct skin feeding assays were strongly correlated (p<0.0001). Replacing the plasma of the gametocyte donor with malaria naïve control serum resulted in higher mosquito infection rates compared to own plasma (OR 1.92, 95% CI 1.68–2.19) while the infectiousness of gametocytes may be reduced during the replacement procedure (OR 0.60, 95% CI 0.52–0.70).ConclusionsDespite a higher efficiency of direct skin feeding assays, membrane feeding assays appear suitable tools to compare the infectiousness between individuals and to evaluate transmission-reducing interventions. Several aspects of membrane feeding procedures currently lack standardization; this variability makes comparisons between laboratories challenging and should be addressed to facilitate future testing of transmission-reducing interventions.

We applied the Mann-Kendall (MK) test and Bayesian model to systematically explore trends and abrupt changes of the precipitation series in the Pearl River basin. The results showed that no significant trends were detected for annual precipitation and summer or winter precipitation totals. Significant negative trends were identified for the number of rainy days across the Pearl River basin; significant positive trends were observed regarding precipitation intensity (PI). In particular, the precipitation totals and frequencies of extremely high precipitation events are subject to significant positive trends. In addition, the number of extremely low precipitation events was also increasing significantly. Factors affecting the changes in precipitation patterns are the weakening Asian monsoon and consequently increasing moisture transport to Southern China and the Pearl River basin. In summary, the main findings of this study are: (1) increased precipitation variability and high-intensity rainfall was observed though rainy days and low-intensity rainfall have decreased, and (2) the amount of rainfall has changed little but its variability has increased over the time interval divided by change points. These finds indicate potentially increased risk for both agriculture and in locations subject to flooding, both urban and rural, across the Pearl River basin.

Abstract:Coupled with poor urban development, the increasing urban population of many Sub-Saharan African countries is subject to recurrent severe flooding episodes. In response to these flood events, while the focus is often put on slums and precarious urban settings, the social implications of these floods affect a variety of social classes. Presenting a case study of Cocody, a district of Abidjan, Côte d'Ivoire, known to have the country's highest number of flood-impacted people, this paper evaluates the social vulnerability of urban Côte d'Ivoire to flooding using the MOVE framework. The MOVE framework (Method for the Improvement of Vulnerability Assessment in Europe) has successfully been used in European contexts to assess social vulnerability of urban areas to geo-environmental disasters such floods. It helped assess the major factors involved in the social vulnerability to urban flooding and to have a good appreciation of the spatial distribution of areas that are vulnerable to urban flood. By taking this framework to the local context, relevant indicators were developed and GIS applications were used to assess spatially the relative social vulnerability of Cocody sub-districts to urban flooding. The results revealed that many sub-districts of Cocody are highly vulnerable to urban floods. Exposure and susceptibility are components that are found to have high influence on vulnerability to flood hazard in the district of Cocody. Their respective indicators need to be addressed properly in order to increase residents' resilience to urban flooding. The MOVE theoretical framework can be applied in Africa by contextualizing the vulnerability by using local indicators.

The use of a panel of polyclonal antisera and monoclonal antibodies (MAbs) raised against West African isolates of rice yellow mottle virus (RYMV) in ELISA resulted in separation of 73 RYMV isolates into three distinct serogroups. Using a set of differential rice varieties, the serogroups could be correlated to two RYMV pathotypes. A relationship was found between serological properties of the RYMV isolates and their probable ecological origin. It was concluded that RYMV isolates originating in closely related agroecological zones displayed variability in coat protein and pathogenicity. This should be taken into account in developing tolerant or resistant rice varieties.

Hemoglobin variants C and S protect against severe malaria but their influence on parameters not directly linked to disease severity such as gametocyte carriage and infection chronicity is less well understood. To assess whether these infection-related phenotypes depend on the host hemoglobin genotype, we followed 500 Malian individuals over 1–2 years and determined their parasitological status during monthly visits and incidental clinical episodes. While adults heterozygous for hemoglobin S mutation were less often parasitemic compared to AA adults (odds ratio [OR] 0.50 95% confidence interval [CI] 0.31–0.79, P = 0.003), schoolchildren (but not toddlers or adults) with AC genotype carried parasites, including gametocytes, more often than their AA counterparts (OR 3.01 95% CI 1.38–6.57, P = 0.006). AC children were also likelier to be parasite-positive during the dry season, suggesting longer infections, and were more infectious in mosquito skin feeding assays than AA children. Notably, AC school-aged children, who comprise ~5% of the population, harbor a third of infections with patent gametocytes between May and August, when transmission transitions from very low to intense. These findings indicate that schoolchildren with hemoglobin C mutation might contribute disproportionately to the seasonal malaria resurgence in parts of West Africa where the HbC variant is common.