Characterization of rhizobia that nodulate Arachis pintoi by RAPD analysis

Pinto, Patrícia Pereira; Paiva, Edílson; Purcino, H. M. A.; Passos, Raul Vinícius Magalhães; Sá, Nadja Maria Horta de

doi:10.1590/s1517-83822004000200009

Cited by 6 publications

(2 citation statements)

References 10 publications

(14 reference statements)

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“…Thus, this result is further evidence that PCR-RAPD is a useful tool to conduct persistence and competitiveness studies in rhizobia [30][31][32]. Also, it is in agreement with who tested 28 of indigenous rhizobia nodulating chickpea in India using RFLP to classify their isolates and who found that all RAPD primers detected one or more polymorphic DNA fragments among the studied rhizobia species and that RAPD is a very discriminative and efficient method for differentiating and studying genetic diversity of Rhizobium.…”

Section: Discussionsupporting

confidence: 65%

Diversity of Rhizobia Nodulating Faba Bean (Vicia faba) Growing in Egypt

Hassan¹

2015

J Microb Biochem Technol

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Citation: Hassan MM, Fahmi AI, Eissa RA, Nagaty HH (2015) Diversity of Rhizobia Nodulating Faba Bean (Vicia faba) Growing in Egypt. J Microb Biochem Technol 7: 152-159. doi:10.4172/1948-5948.1000197 Copyright: © 2015 Hassan MM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited AbstractThe aim of this work was to characterize and to describe the diversity and phylogeny of rhizobial bacteria associated to nodules of faba bean in four different geographical regions of north Egypt. Eight rhizobial isolates from healthy faba bean roots were isolated. They were identified as Rhizobium leguminosarum morphologically. They showed sensitivity to Kanamycin, Neomycin and Sulphemethooxazole antibiotics. Mannitol was the best source of carbon source for their growth. However, two isolates Rl. 2 and Rl. 10 indicated better tolerance to high NaCl concentrations than the other isolates and their plasmid profiles contained additional large plasmid with molecular weight about 23 kb. A relationship between salt tolerance and extra plasmid was suggested. Analysis of similarity among rhizobial isolates by using the RAPD-PCR technique showed a high level of genetic polymorphism, grouping the rhizobial isolates into two different clusters. These clusters reflexed the similarity among genotype of isolates independent of their geographic locations. 16S rDNA sequences of three representing isolates were determined and were aligned and compared with the 16S rDNA sequences of other members of the family Rhizobiaceae available in the Gene Bank database. The obtained dendogram indicated that the isolates belonging to Rhizobium leguminosarum biovar viciaen.

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“…However, this finding is not in agreement with the current knowledge that the peanut crop is usually nodulated by slow-growing rhizobia (Bradyrhizobium). Pinto et al (2004) confirmed the predominance of slowgrowing bacteria in Arachis pintoi grown in the soils of the Brazilian Cerrado (savannah). Taken together, these results indicate that peanut can form symbiosis with bacteria harboring diverse culture characteristics.…”

Section: Discussionsupporting

confidence: 66%

Phenotypic and molecular characteristics of rhizobia isolated from nodules of peanut (Arachis hypogaea L.) grown in Brazilian Spodosols

Lyra¹,

Freitas

Silva

et al. 2013

Afr. J. Biotechnol.

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Peanut (Arachis hypogaea L.) is an important crop that can fix nitrogen through symbiosis with rhizobia. Rhizobia populations with diverse characteristics than those traditionally described as peanut microsymbionts have been found in tropical soils. With the objective of studying the diversity and phylogeny of these rhizobia, 22 bacterial strains were isolated from nodules of seven peanut varieties grown in Spodosols of Pernambuco State, Brazil. The isolates were examined in culture medium by means of some of their phenotypic characteristics and tested for intrinsic antibiotic resistance (IAR). DNA profiles were determined with the BOX-PCR and compared with 19 reference strains. All isolates showed rapid growth, and most of them acidified the culture medium. In general, high antibiotic resistance was observed to penicillin G, chloramphenicol and tetracycline and susceptibility was observed to neomycin, erythromycin and rifampicin. The analysis of their phenotypic characteristics, that is, colony morphology and IAR, provided little information about the phylogeny of the isolates. However, using compilation of phenotypic and molecular characteristics, we were able to observe a great diversity of these rhizobia and to reveal the presence of new species.

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Characterization of rhizobia that nodulate Arachis pintoi by RAPD analysis

Cited by 6 publications

References 10 publications

Diversity of Rhizobia Nodulating Faba Bean (Vicia faba) Growing in Egypt

Phenotypic and molecular characteristics of rhizobia isolated from nodules of peanut (Arachis hypogaea L.) grown in Brazilian Spodosols

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