Diversity of Rhizobia Nodulating Faba Bean (Vicia faba) Growing in Egypt

Abstract

Citation: Hassan MM, Fahmi AI, Eissa RA, Nagaty HH (2015) Diversity of Rhizobia Nodulating Faba Bean (Vicia faba) Growing in Egypt. J Microb Biochem Technol 7: 152-159. doi:10.4172/1948-5948.1000197 Copyright: © 2015 Hassan MM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited AbstractThe aim of this work was to characterize and to describe the diversity and phylogeny of rhizobial bacteria associated to nodules of faba bean in four different geographical regions of north Egypt. Eight rhizobial isolates from healthy faba bean roots were isolated. They were identified as Rhizobium leguminosarum morphologically. They showed sensitivity to Kanamycin, Neomycin and Sulphemethooxazole antibiotics. Mannitol was the best source of carbon source for their growth. However, two isolates Rl. 2 and Rl. 10 indicated better tolerance to high NaCl concentrations than the other isolates and their plasmid profiles contained additional large plasmid with molecular weight about 23 kb. A relationship between salt tolerance and extra plasmid was suggested. Analysis of similarity among rhizobial isolates by using the RAPD-PCR technique showed a high level of genetic polymorphism, grouping the rhizobial isolates into two different clusters. These clusters reflexed the similarity among genotype of isolates independent of their geographic locations. 16S rDNA sequences of three representing isolates were determined and were aligned and compared with the 16S rDNA sequences of other members of the family Rhizobiaceae available in the Gene Bank database. The obtained dendogram indicated that the isolates belonging to Rhizobium leguminosarum biovar viciaen.

Characterization of Indigenous Rhizobia Strains Associated to Soybean [Glycine max (L.) Merrill] in Benin

“…But sucrose was the least carbon source utilized by isolates. This is in line with the result of other studies [20,7,26]. According to Singh et al [27], rhizobial isolates may not grow on lactose, however this is one of the best carbon sources for all isolates including the reference strain.…”

Genetic characterization of some rhizobia isolates from various legumes

“…were classified as R. leguminosarum, suggesting that this strain predominates in the four legumes: faba bean (V. faba), clover (T. alexandrinum ), pea (P. sativum ), and lentil (L. culinaris ) in the seven governorates of Egypt under study. In conclusion, these findings demonstrated that the rhizobial isolates in the present study are closely related and may have a recent common ancestral origin (Hassan et al, 2015). This may also be due to a high degree of conservation of the 16S rRNA molecule (Berrada et al, 2012).…”

“…Ismail et al (2013) also indicated that rhizobium isolated from the root nodules of broad beans (V. faba L.) growing in 10 locations in Egypt were R. leguminosarum, and they found a narrow genetic distance among isolates. Fahmi et al (2011), Hassan et al (2015, and Ismael et al (2018) supported the same results on V. faba. The narrow genetic variations could also possibly be due to the conserved nature of the 16S rRNA gene, which could not discriminate between closely related rhizobia species (Berrada et al, 2012).…”

“…In the last few decades, there has been a growing interest in environmentally friendly sustainable agricultural practices, thus increasing the role of biofertilizers such as rhizobia, which can decrease the need for chemical fertilizers and thus reduce adverse 180 negative environmental effects and help to save money. Therefore, information on the distribution and genetic variation of native rhizobial isolates would aid in selecting novel rhizobial strains that could be developed and used as biofertilizers in legume production (Eissa et al, 2009;Hassan et al, 2015). Rhizobia-legume symbiosis is of special importance for agricultural ecosystems, annually producing 50% of the total biological nitrogen fixation worldwide (Yadav and Verma, 2014).…”

Eco-physiological and physiological characterization of cowpea nodulating native rhizobia isolated from major production areas of Ethiopia

“…This result agrees with the report of Stowers and Elkan (1984) who reported that all 25 isolates from cowpea showed growth on different carbon sources such as glucose, fructose, galactose and arabinose using them as good energy source and showed limited growth response on lactose and maltose. However, Argaw (2007) and Hassan et al (2015) reported that rhizobia can also utilize lactose as carbon sources.…”