Characterization of renal ischemia-reperfusion injury in rats

Williams, Patricia D.; Lopez, Henry; Britt, Deborah E.; Chan, Christine; Ezrin, Alan M.; Hottendorf, Rod

doi:10.1016/s1056-8719(96)00141-4

Cited by 138 publications

(87 citation statements)

References 5 publications

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“…22,23 In the control group the BUN and creatinine levels remained high till the 7 th postoperative day even though exhibiting a tendency to return to normal values. In the group treated with CPZ the levels of BUN and creatinine were lower than in the control group in the period 1 st -4 th days, and at the 7 th day their values return to normal.…”

Section: Discussionmentioning

confidence: 97%

Renal ischemia and reperfusion injury: influence of chorpromazine on renal function and lipid peroxidation

et al. 2008

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Purpose: To evaluate the influence of chlorpromazine (CPZ) on renal function and lipid peroxidation in a rat model of kidney ischemia/reperfusion injury. Methods: Forty eight Wistar rats underwent a laparotomy for hilar clamping of left kidney with a bulldog clamp for 60 minutes followed by organ reperfusion and contralateral nephrectomy. Of these, 26 received 3mg/kg of CPZ intravenously 15 minutes before renal ischemia (G-E) while the remaining 22 were used as ischemic control group (G-C). Eleven rats of G-E and 8 of G-C were followed for blood urea nitrogen and creatinine determinations before renal ischemia and at 1 st , 4 th and 7 th postoperative days. Samplings of left renal tissue were obtained at 5 minutes (5 rats from each group) and 24 hours (9 G-C and 10 of G-E) of reperfusion for malondialdehy (MDA) content determination. Controls of renal MDA content were determined in kidneys harvested from 6 additional normal rats. Results: Acute renal failure occurred in all animals but levels of BUN and creatinine were significantly lower in G-E (p<0.001). MDA content rose strikingly at 5 minutes of reperfusion in both groups (p>0.05) and returned near to normal levels 24 hours later. Conclusion: CPZ conferred partial protection of renal function to kidneys submitted to ischemia/reperfusion injury that seems to be not dependent on inhibition of lipid peroxidation. Key words:Renal Ischemia/Reperfusion. Lipid Peroxidation. Malondialdehyde. Chlorpromazine. Free Radicals. RESUMOObjetivo: avaliar a influência da clorpromazina (CPZ) na função renal e na peroxidação lipídica num modelo de lesão de isquemia/reperfusão renal em ratos. Métodos: 48 ratos Wistar foram submetidos à laparotomia para clampamento da artéria renal esquerda durante 60 minutos, seguido da reperfusão e nefrectomia contralateral. Destes animais, 26 receberam 3 mg/ kg de CPZ intravenosa 15 minutos antes da isquemia renal (G-E), sendo os 22 animais restantes utilizados como grupo controle isquêmico (G-C). Em 11 ratos do G-E e 8 do G-C foi feita a dosagem de uréia e creatinina sérica antes da isquemia renal e no 1º, 4º e 7º dia pós-operatório. Amostras de tecido do rim esquerdo foram obtidas aos 5 minutos (5 ratos de cada grupo) e 24 horas após reperfusão (9 G-C e 10 G-E) para dosagem de malondialdeído (MDA). Valores controle para níveis de MDA foram obtidos em rins retirados de 6 ratos normais. Resultados: insuficiência renal aguda ocorreu em todos os animais mas os níveis séricos de uréia e creatinina foram significativamente menores no G-E (p<0,001). Os níveis de MDA apresentaram elevação acentuada na avaliação aos 5 minutos de reperfusão em ambos os grupos (p<0,05), retornando a valores próximos aos normais na avaliação com 24 horas. Conclusão: a CPZ conferiu proteção parcial da função renal aos rins submetidos à lesão de isquemia e reperfusão, aparentemente independente da inibição da peroxidação lipídica.

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Section: Discussionmentioning

confidence: 97%

Renal ischemia and reperfusion injury: influence of chorpromazine on renal function and lipid peroxidation

et al. 2008

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“…Regeneration and remodeling of the kidney results in recovery of renal function and morphology by tubular epithelial cell replacement (2,3). However, tubulointerstitial remodeling, as a result of an uncontrolled balance between synthesis and degradation of extracellular matrix (ECM) proteins, also can result in tubulointerstitial fibrosis (4,5), which is an important risk factor for progressive renal function loss (6).…”

mentioning

confidence: 99%

Bone Marrow–Derived Myofibroblasts Contribute to the Renal Interstitial Myofibroblast Population and Produce Procollagen I after Ischemia/Reperfusion in Rats

Broekema¹,

Harmsen²,

Luyn³

et al. 2007

Journal of the American Society of Nephrology

157

130

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Bone marrow-derived cells (BMDC) have been proposed to exert beneficial effects after renal ischemia/reperfusion injury (IRI) by engraftment in the tubular epithelium. However, BMDC can give rise to myofibroblasts and may contribute to fibrosis. BMDC contribution to the renal interstitial myofibroblast population in relation to fibrotic changes after IRI in rats was investigated. A model of unilateral renal IRI (45 min of ischemia) was used in F344 rats that were reconstituted with R26-human placental alkaline phosphatase transgenic BM to quantify BMDC contribution to the renal interstitial myofibroblast population over time. After IRI, transient increases in collagen III transcription and interstitial protein deposition were observed, peaking on days 7 and 28, respectively. Interstitial infiltrates of BMDC and myofibroblasts reached a maximum on day 7 and gradually decreased afterward. Over time, an average of 32% of all interstitial ␣-smooth muscle actin-positive myofibroblasts coexpressed R26-human placental alkaline phosphatase and, therefore, were derived from the BM. BMD myofibroblasts produced procollagen I protein and therefore were functional. The postischemic kidney environment was profibrotic, as demonstrated by increased transcription of TGF-␤ and decreased transcription of bone morphogenic protein-7. TGF-␤ protein was present predominantly in interstitial myofibroblasts but not in BMD myofibroblasts. In conclusion, functional BMD myofibroblasts infiltrate in the postischemic renal interstitium and are involved in extracellular matrix production.

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“…In the present study, as in the studies of Williams et al [14] and Cochrane et al, [15] ischemic duration of 45 minutes and reperfusion duration of 4 hours, which allows for observation of earliest renal damage, was performed. The fact that histomorphological damage scores were significantly higher in the IR group than in the Sham group indicated that adequate renal IR damage can be obtained using this model.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of dexmedetomidine and remote ischemic preconditioning on renal ischemia reperfusion injury in rats

Balcı¹

2017

Ulus Travma Acil Cerrahi Derg

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Characterization of renal ischemia-reperfusion injury in rats

Cited by 138 publications

References 5 publications

Renal ischemia and reperfusion injury: influence of chorpromazine on renal function and lipid peroxidation

Renal ischemia and reperfusion injury: influence of chorpromazine on renal function and lipid peroxidation

Bone Marrow–Derived Myofibroblasts Contribute to the Renal Interstitial Myofibroblast Population and Produce Procollagen I after Ischemia/Reperfusion in Rats

Protective effects of dexmedetomidine and remote ischemic preconditioning on renal ischemia reperfusion injury in rats

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