Characterization of Proteases Production by Varying Carbon Sources from Bacillus subtilis Isolated from Agriculture Soil of Lalitpur Dist. (U.P.)

Abstract

Proteases are class of proteolytic enzymes that accounts for approximately 45% of the total enzyme sales in various pharmaceuticals industrial ranging from potential agent for curing AIDS, treatments of inflammation and virulent wounds, and remodelling of various proteins. Protease has a great boon in food industry, leather industry, and biomining of metals like silver from ores along with bioremediation activity of waste. Microbes serve as a preferred source for proteases and major the contributor to this is derived from Bacillus strains. Bacillus subtilis belongs to this class is the major producer of proteases enzyme commercially. The major issue being the high cost of substrate that makes the overall production cost highly expensive. An attempt was made to formulate media using varied carbon sources to optimize media to maximize the production of proteases. Lactose was found to be an ideal carbon source among the selected carbon sources that yielded maximum biomass of 160 mg/ml. Sucrose with a yield of 132.5 mg/ml and glycerol 129.3 mg/ml which was comparatively less then lactose while the other carbon sources like maltose, glucose, cellulose, starch were found to be poor in comparison to lactose.

Characterization of Proteases Production by Varying Nitrogen Sources from Bacillus subtilis Isolated from Agriculture Soil of Lalitpur Dist. UP

“…Of the six strains of Bacillus subtilis namely BS-1, BS-2, BS-3, BS-4, BS-5 and BS-6 were isolated and identified BS-5 was selected on the basis of its proteolytic activity. Bacillus subtilis is known to utilize crude substrates for protease production observed by Murab et al [12] . The type and availability of nitrogenous precursor in the medium plays a crucial role in metabolism of extracellular enzyme as suggested by Kumar et al [5] and Reddy et al [17] .…”

“…[11] Six strains of Bacillus subtilis were identified namely BS-1, BS-2, BS-3, BS-4, BS-5 and BS-6 were isolated and identified based on cellular morphology, growth condition, and biochemical analysis. [12] For protease screening, casein agar medium (g/l) (peptic digest of animal tissue, 5.0; beef extract, 1.5; yeast extract, 1.5; sodium chloride, 5.0, agar, 15, casein, 10 and 0. 0015% (w/v) BCG-Bromocresol Green Dye) was prepared and streaked with bacterial isolate (Bacillus subtilis) and incubated at 37°C for 48 hrs.…”