Characterization of Proteases Production by Varying Nitrogen Sources from Bacillus subtilis Isolated from Agriculture Soil of Lalitpur Dist. UP

Abstract

Proteases is among the largest groups of industrial enzymes that also has the potential to contribute in the development of high value added products due to their characteristic nature that aids in digestion. Protease account for about 60% of the total worldwide sale of enzymes and is widely used in several industries ranging from silk industry, leather tanning, meat processing, organic fertilizers, diary and bioleaching. Bacteria produce a variety of proteolytic enzymes. Among them a major contributor of proteases producers is Bacillus subtilis. An attempt was made to formulate media using varied nitrogen sources to optimize media for maximum production of proteases. It was observed that media supplemented with soya meal as a nitrogen source had maximum biomass yield of 135 mg/ml while Tryptone supplemented media yielded 115.6 mg/ml and peptone supplemented media yielded only 101 mg/ml which was comparatively less than soya meal while the other nitrogen sources supplemented media were found to be poor in comparison to that supplemented by soya meal extract.