Characterization of phenolic compounds in wild medicinal flowers from Portugal by HPLC–DAD–ESI/MS and evaluation of antifungal properties

Abstract

In the present work, the phenolic compounds of Castanea sativa, Filipendula ulmaria and Rosa micrantha flowers from Northeastern Portugal were characterized by HPLC-DAD-ESI/MS. Furthermore, it was performed a screening of their antifungal potential against Candida species (C. albicans, C. glabrata, C. parapsilosis and C. tropicalis).C. sativa sample gave the highest amount of phenolic compounds (18973 ± 40 µg/g, fw)and hydrolysable tannins (14873 ± 110 µg/g). The highest amounts of phenolic acids (569 ± 20 µg/g) and flavonoids (6090 ± 253 µg/g) were obtained in F. ulmaria and R. micrantha samples, respectively. Hydrolysable tannins (e.g. tri and digalloyl HHDP glucose) were the main group of phenolic compounds in C. sativa and F. ulmaria samples, while flavonoids (e.g. (+)-catechin and procyanidin dimers and trimers) were the most abundant group in R. micrantha. Thus, the stronger effect showed by this latter against all the Candida species (MIC ≤ 0.155 mg/mL) and, particularly its fungicide effects in C. glabrata, might be related to the mentioned flavonoids that were inexistence in the other samples.

Antifungal Activity of Phenolic Compounds Identified in Flowers from North Eastern Portugal Against Candida Species

“…The extraction, identification and quantification of phenolic compounds from flowers of Castanea sativa, Filipendula ulmaria, Rosa micrantha [24] and Cytisus multiflorus [25], and fresh leaves of Cistus ladanifer [26] were previously described by the authors using a high-performance liquid chromatography-diode array detector/electrospray source mass spectrometer. This work was focused on four different phenolic compounds that seemed more promising against Candida species: one phenolic acid (gallic acid) and three flavonoids (catechin, luteolin and quercetin).…”

Differential susceptibility of Morettini pears to blue mold caused by Penicillium expansum

“…Moreover, the identification of several additional glycosides of quercetin and isorhamnetin is highlighted with two quercetin glycosides derivatives: quercetin-manonylhexoxide (peak 7, m/z 549/551) and quercetin-acetyl hexoside (peak 8, m/z 505), and four compounds that involved isorhamnetin glycosides and derivatives, namely isorhamnetin-O-rhamnose-glucoside (peak 9, m/z 625) isorhamnetin-3-O-glucoside (peak 10, m/z 477/479), isorhamnetin-manonyl hexoside (peak 11, m/z 563/565), and isorhamnetin-acetyl hexoside (peak 12, m/z 519) (Schieber et al 2002, Barros et al 2013 ( Table 3). Other phenolic compounds identified in the pear extracts are phenolic acids, namely quinic acid (peak 1, m/z 191) and caffeoylquinic acid (peak 2, m/z 353); and one procyanidin dimer (peak 3, m/z 577) (Long-Ze and Harnyl, 2007).…”

Flower extracts of Filipendula ulmaria (L.) Maxim inhibit the proliferation of the NCI-H460 tumour cell line

“…These results will need to be further confirmed with other specific assays for DNA damage. In fact, such a result is surprising considering the fact that the flowers from this plant are known for their rich antioxidants content (Barros et al, 2011;Barros et al, 2013). Nevertheless, those antioxidants might not be enough to avoid DNA damage, as least under the tested in vitro conditions.…”

“…Filipendula ulmaria (L.) Maxim, commonly known as meadowsweet, is an interesting example of useful plants reported by several ethnobotanical surveys and can be found not only in the Iberian Peninsula, but also in other regions of most of Europe and Western Asia, such as Poland and Russia (Barros et al, 2013). Homemade remedies prepared from this species have been described since the late 16 th and 17 th centuries.…”

“…Regarding the phenolic compounds, phenolic acids (caffeic acid derivatives and gallic acid), flavonols (glycosides of quercetin and isorhamnetin) and ellagitannins (digalloylhexahydroxydiphenol-glucose and trigalloyl digalloyl-hexahydroxydiphenol-glucose) have been described (Barros et al, 2013).…”