Flower extracts of Filipendula ulmaria (L.) Maxim inhibit the proliferation of the NCI-H460 tumour cell line

Lima, M. João; Sousa, Diana; Lima, Raquel T.; Carvalho, Ana María; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena

doi:10.1016/j.indcrop.2014.05.009

Cited by 18 publications

(12 citation statements)

References 21 publications

(21 reference statements)

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“…The effect of extract/fractions on cell cycle distribution was assessed by flow cytometry after propidium iodide staining [ 38 ]. MDA-MB-231 cells (1 × 10 5 cells/well) were treated with NJM and NJDE at 20 μg/mL and NJPE and NJEA at 35 μg/mL for 48 h. Cells in the control group received only media containing 0.1% DMSO and doxorubicin was used as positive control.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma

Chaudhary

Chandrashekar

Pai

et al. 2015

BMC Complement Altern Med

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BackgroundNardostachys jatamansi DC is a Himalayan medicinal herb that has been described in various traditional systems of medicine for its use in cancer. In view of its traditional claims, and chemical constituents, antioxidant and anticancer activities were evaluated in breast carcinoma.MethodsPetroleum ether (NJPE), methanol extract (NJM) and subsequent diethyl ether (NJDE), ethyl acetate (NJEA) and aqueous (NJAQ) fractions of roots and rhizomes of N. jatamansi were prepared. Total phenolic, flavonoid content, and antioxidant activities were determined using suitable methods. Antiproliferative activity was assessed in estrogen receptor (ER)-positive (MCF-7) and ER-negative breast carcinoma (MDA-MB-231) cells by MTT and SRB assay. Cell cycle analysis, Hoechst staining, and clonogenic assay were employed to determine the mode of antiproliferative and pro-apoptotic activity in MDA-MB-231 cells.ResultsNJM/fractions exhibited prominent antioxidant activity with significant correlation between phenolic content and ABTS (IC50) scavenging (R = −0.9680, P < 0.05), and total antioxidant capacity (R = 0.8396, P > 0.05). In MTT assay, NJM exhibited the highest antiproliferative activity (IC50: 58.01 ± 6.13 and 23.83 ± 0.69 μg/mL in MCF-7 and MDA-MB-231 respectively). Among the fractions, NJPE and NJDE were found to be most potent in MCF-7 (IC50: 60.59 ± 4.78 μg/mL) and MDA-MB-231 (IC50: 25.04 ± 0.90 μg/mL) cells respectively. Statistical analyses revealed NJM and NJDE exhibited significantly higher (P < 0.05) cytotoxicity in MDA-MB-231 cells. Cell cycle analysis demonstrated that NJM, NJPE and NJEA caused G2/M arrest while NJDE caused G0/G1 phase arrest in MDA-MB-231 cells. Further, NJM/fractions induced significant (P < 0.001) cell death by apoptosis characterized by apoptotic morphological changes in Hoechst staining and inhibited long-term proliferation (P < 0.001) of MDA-MB-231 cells in clonogenic assay. Lupeol and β-sitosterol were identified as anticancer principles in NJM/fractions by HPTLC.ConclusionOur results suggest that NJM/fractions possess significant antiproliferative potential which is mediated through cell cycle perturbation and pro-apoptotic effects in MDA-MB-231 cells. Moreover, this study highlights the antioxidant potential of NJM/fractions which can be attributed to the presence of phenols. NJDE emerged as the most potent fraction and further mechanistic and phytochemical investigations are under way to identify the active principles.

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Section: Methodsmentioning

confidence: 99%

Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma

Chaudhary

Chandrashekar

Pai

et al. 2015

BMC Complement Altern Med

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“…In the case of MCF-7 and NCI-H460 cells, the appropriate cellular density was 5.0 × 10 4 cells per well 17,18 and for the AGS cells the cellular density was 7.5 × 10 4 cells per well. 19 Two plates were prepared, one to be analysed at the time of addition of the extracts to cells (T0 plate) and the other to be analysed 48 hours after treating the cells with the extracts.…”

Section: Cell Growth Inhibition Assaymentioning

confidence: 99%

Melissa officinalisL. ethanolic extract inhibits the growth of a lung cancer cell line by interfering with the cell cycle and inducing apoptosis

et al. 2018

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Melissa officinalis is a plant from the family Lamiaceae, native in Europe particularly in the Mediterranean region. Given our interest in identifying extracts and compounds capable of inhibiting tumor cell growth, and given the antioxidant content and the high consumption of Melissa officinalis in Portugal, this study aimed to test the tumor cell growth inhibitory activity of five different extracts of this plant (aqueous, methanolic, ethanolic, hydromethanolic and hydroethanolic) in three human tumor cell lines: MCF-7, AGS and NCI-H460. All extracts decreased cell growth in all cell lines in a concentration-dependent manner. The ethanolic extract was the most potent one, presenting a GI 50 concentration of approximately 100.9 µg mL −1 in the NCI-H460 lung cancer cells. This extract was characterized by LC-DAD-ESI/MS regarding its phenolic composition, revealing rosmarinic acid as the most abundant compound. The GI 75 concentration of this extract affected the cell cycle profile of these cells. In addition, both the GI 50 and the GI 75 concentrations of the extract induced cellular apoptosis. Moreover, treatment of NCI-H460 cells with this extract caused a decrease in pro-caspase 3 and an increase in p53 levels. This study emphasizes the relevance of the study of natural products as inhibitors of tumor cell growth.

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“…For experiments in cultured cells, the TUNEL assay was carried out using the ‘‘in situ cell death detection kit—fluorescein’’ (Roche, Boulogne-Billancourt Cedex, France) as previously described [20,66]. For the analysis of apoptosis in tumor xenograft tissue sections, the TUNEL assay kit “Apoptag Red in situ apoptosis detection kit” (Chemicon, Temecula, CA, USA) was used as previously described [67].…”

Section: Methodsmentioning

confidence: 99%

The Antitumor Activity of a Lead Thioxanthone is Associated with Alterations in Cholesterol Localization

et al. 2018

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The search for novel anticancer small molecules and strategies remains a challenge. Our previous studies have identified TXA1 (1-{[2-(diethylamino)ethyl]amino}-4-propoxy-9H- thioxanthen-9-one) as a hit compound, with in vitro antitumor potential by modulating autophagy and apoptosis in human tumor cell lines. In the present study, the mechanism of action and antitumor potential of the soluble salt of this molecule (TXA1.HCl) was further investigated using in vitro and mouse xenograft tumor models of NSCLC. Our results showed that TXA1.HCl affected steroid biosynthesis, increased RagD expression, and caused abnormal cellular cholesterol localization. In addition, TXA1.HCl treatment presented no toxicity to nude mice and significantly reduced the growth of human NSCLC cells xenografts in mice. Overall, this work provides new insights into the mechanism of action of TXA1, which may be relevant for the development of anticancer therapeutic strategies, which target cholesterol transport.

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Flower extracts of Filipendula ulmaria (L.) Maxim inhibit the proliferation of the NCI-H460 tumour cell line

Abstract: Filipendula ulmaria (L.) Maxim (meadowsweet) is a popular medicinal species that can be found throughout most Europe and Asia. The plant is known for its rich antioxidants content, having compounds such as flavonoids and ascorbic acid. Therefore, the aim of

Cited by 18 publications

References 21 publications

Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma

Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma

Melissa officinalisL. ethanolic extract inhibits the growth of a lung cancer cell line by interfering with the cell cycle and inducing apoptosis

The Antitumor Activity of a Lead Thioxanthone is Associated with Alterations in Cholesterol Localization

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