Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis

Chung, Young Bae; Lee, Mejeong; Yang, Hyun; Chung, Byung Suk; Lee, Shun Yu; Choi, Min Ho; Hong, Sung Tae

doi:10.3347/kjp.2002.40.2.83

Cited by 12 publications

(6 citation statements)

References 8 publications

(20 reference statements)

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“…The 7-kDa band was actually a mixture of 7-and 8-kDa bands which were hardly differentiated by SDS-PAGE. The 8-kDa band was observed mainly in the tegument (Chung et al, 2002). That may be an important point because some tegumental tissues were contaminated during the organ preparation of the uterus and testis.…”

Section: Discussionmentioning

confidence: 99%

Organ-specific antigens of Clonorchis sinensis

Chung

Choi

et al. 2004

Korean J Parasitol

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This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7-and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26-and 28-kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43-and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.

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Section: Discussionmentioning

confidence: 99%

Organ-specific antigens of Clonorchis sinensis

Chung

Choi

et al. 2004

Korean J Parasitol

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“…These were found to be concentrated mainly in the tegument and the seminal receptacle by immunohistochemistry (Chung et al, 2002). The protein band possibly resulted from the partition of a larger complexed protein.…”

Section: Discussionmentioning

confidence: 99%

“…Especially cross-reactivity of ESA with serum specimens of opisthorchiasis, schistosomiasis and paragonimiasis was reduced compared to that of CA. C. sinensis ESA was found to include proteins of 7-8, 12.5, and 30 kDa (Kim, 1998), and the 7 or 8 kDa protein predominated (Chung et al, 2002). ESA has been suggested to be a valuable candidate diagnostic antigen of active clonorchiasis in rabbits and humans (Kim, 1994(Kim, & 1998.…”

Section: Discussionmentioning

confidence: 99%

Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA

Choi

Park

et al. 2003

Korean J Parasitol

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Abstract:Although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis by ELISA using crude antigen is now widely used because of its convenience. However, ELISA diagnosis still suffers from cross-reactions, and therefore there is a need to improve the present conventional ELISA. The present study was undertaken to evaluate the diagnostic value of ELISA using excretory-secretory antigen (ESA) instead of crude antigen (CA) of Clonorchis sinensis. The diagnostic sensitivity of ELISA using excretory-secretory antigen was 92.5%, which was higher than that of ELISA using crude Clonorchis sinensis antigen (88.2%). In addition, the specificity of excretory-secretory antigen was found 93.1% while that of crude antigen was 87.8%. In summary, Clonorchis sinensis ESA was found to be a better serodiagnostic antigen than CA for ELISA.

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“…The fish were digested by using artificial gastric juice (0.5% pepsin in 1% HCl) and the metacercariae collected as described previously (Chung et al 2002). Fresh metacercariae were washed three times in sterile physiological saline before being used in the excystment experiments.…”

Section: Isolation Of C Sinensis Metacercariaementioning

confidence: 99%

The involvement of the cysteine proteases of Clonorchis sinensis metacercariae in excystment

Chung

et al. 2004

Parasitology Research

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The effects of trypsin, bile, trypsin-bile, pepsin, dithiothreitol (DTT) and metacercarial excretory-secretory product (ESP) on the in vitro excystment of Clonorchis sinensis metacercariae were investigated. The majority of metacercariae excysted immediately in trypsin-bile in PBS solution, a process which was complete after 30 min of incubation. When incubated in metacercarial ESP in PBS, excystment was potentiated in the presence of 5 mM DTT, but was inhibited dose-dependently by a cysteine protease inhibitor, iodoacetic acid. Two active protease bands of 28 and 40 kDa were identified in the ESP of metacercariae by gelatin substrate SDS-PAGE. Scanning electron microscopy demonstrated that the larvae in solutions of DTT and ESP migrated through a small hole on the metacercarial wall, whereas larvae were liberated by entire wall disruption in trypsin solution. These results suggest that trypsin is a major extrinsic factor of the rapid excystment of C. sinensis metacercariae, and that endogenous cysteine proteases are also involved in metacercarial excystment.

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Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis

Cited by 12 publications

References 8 publications

Organ-specific antigens of Clonorchis sinensis

Organ-specific antigens of Clonorchis sinensis

Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA

The involvement of the cysteine proteases of Clonorchis sinensis metacercariae in excystment

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