Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA

Choi, Min Ho; Park, Il Chan; Li, Shunyu; Hong, Sung Tae

doi:10.3347/kjp.2003.41.1.35

Cited by 78 publications

(68 citation statements)

References 13 publications

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“…In the present study S. spindale ESA had not exhibited cross reaction with amphistome positive sera in Dot-ELISA. The high specificity of ESA in serodiagnosis has been observed by several workers for detecting other trematode infections as well viz, Clonorchis sinensis (Choi et al 2003), Paragonimus sp. (Narain et al 2005), F. hepatica (Awad et al 2009), Opisthorchis felineus (Gomez-Morales et al 2013) and Paramphistomum cervi (Anuracpreeda et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Dot-ELISA using different antigens in detecting anti-schistosome antibodies among bovines in field conditions

et al. 2014

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Schistosomosis has been recognised as one of the major parasitic diseases of livestock and human beings. Schistosoma spindale is the major cause of visceral schistosomosis among bovines of Kerala State. Besides pathology in animals, it has been long known that cercariae of S. spindale are a common cause of dermatitis in human beings in Asia. However, detection of this disease based on coprology has underestimated the prevalence of this economically important disease among cattle of the State. An efficient diagnostic tool providing unequivocal evidence of infection in living animals is perhaps, the key to formulate and deliver control measures to the target population. It is also crucial for an enhanced understanding of parasite epidemiology. The utility of excretory-secretory proteins as diagnostic and vaccine candidates for schistosomosis has been a focus of medical research since long. There exists a paucity of information with regard to analysis of ES proteins of S. spindale and their incorporation to develop sensitive and specific serodiagnostic tool. Hence a study was designed to evaluate the efficacy of Dot-ELISA incorporating different antigens of S. spindale and to validate the test under field conditions.

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Section: Discussionmentioning

confidence: 99%

Efficacy of Dot-ELISA using different antigens in detecting anti-schistosome antibodies among bovines in field conditions

et al. 2014

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“…The other sources may include semen fluid, uterine fluid, excretory bladder fluid or the tegument. Since the ES protein was found as a good serodiagnostic antigen to improve specificity, it is an important target of further study (Choi et al, 2003a).…”

Section: Discussionmentioning

confidence: 99%

“…Diagnosis by conventional ELISA, using the crude antigen of C. sinensis, is known to be sensitive and specific, but cross-reactions are known to occur (Choi et al, 2003b). The excretory-secretory (ES) protein of C. sinensis has been suggested to be a more sensitive and specific antigen than crude antigen for diagnosis of human clonorchiasis (Choi et al, 2003a). Several studies reported that 7-, 8-, 26-, 28-, 34-, 37-, 43-, 70-, and 100-kDa bands are candidate antigenic proteins for serodiagnosis of clonorchiasis (Yong et al, 1991;Kim, 1994;Hong et al, 1997;Choi et al, 2003b).…”

Section: Introductionmentioning

confidence: 99%

Organ-specific antigens of Clonorchis sinensis

Chung

Choi

et al. 2004

Korean J Parasitol

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This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7-and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26-and 28-kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43-and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.

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“…One report described the excellent sensitivity and specificity, 92.5% and 100%, respectively, of the ELISA for a population living in an area of nonendemicity (3). However, as laboratory physicians working for a hospital in an area of endemicity, our experiences were that the ELISA seemed to have a low sensitivity and specificity.…”

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confidence: 78%

Performance of an Enzyme-Linked Immunosorbent Assay for Detection of Clonorchis sinensis Infestation in High- and Low-Risk Groups

et al. 2010

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Clonorchis sinensis is still a common parasite in South Korea. An enzyme-linked immunosorbent assay (ELISA) was developed to replace the usual intradermal test, and its performance in an area of endemicity was evaluated. From 2004 to 2006, 182 adults were included. The patient group consisted of 51 adults; 43 patients showed fluke eggs by microscopy, and 8 had their disease diagnosed clinically. The negative-control group included 131 adults: 98 at low risk and 33 at high risk of infestation. Both intradermal testing and ELISA were performed for all patients, and the sensitivity and specificity were evaluated. Statistical analysis for specificity looked at two groups: those at high risk and those at low risk. The sensitivity of the C. sinensis ELISA was 80.4%, while that of the intradermal test was 56.9% (P ‫؍‬ 0.019). The specificities of the C. sinensis ELISA in the low-risk and high-risk groups were 93.9% and 33.3%, respectively, while those of the intradermal test were 85.7% and 30.3%, respectively (P ‫؍‬ 0.0968, P ‫؍‬ 0.9979). The C. sinensis ELISA may be more useful than the intradermal test; however, because of its low specificity, it may not be used independently for the diagnosis of C. sinensis infestation or a prevalence survey in a high-risk group.Clonorchis sinensis is a common parasite found around Nakdong-Gang (river) near Busan, South Korea, a region that has experienced infestation rates of at least 16% and as high as 40% (1, 6, 7). For diagnosis, physicians use imaging, serology, stool microscopy, or an intradermal test, which is simple and requires no special facilities. However, the skin test has several limitations, such as low sensitivity and specificity. Also, interpretation of the results is influenced by subjective reading, and the test cannot distinguish current and past infestations (2).To overcome these deficiencies, an enzyme-linked immunosorbent assay (ELISA) kit for serum antibody detection was developed. One report described the excellent sensitivity and specificity, 92.5% and 100%, respectively, of the ELISA for a population living in an area of nonendemicity (3). However, as laboratory physicians working for a hospital in an area of endemicity, our experiences were that the ELISA seemed to have a low sensitivity and specificity. Therefore, we compared it with the intradermal test in an area of endemicity. MATERIALS AND METHODSPatients. Included were 182 adults who visited Pusan National University Hospital from 2004 to 2006 and who lived in Busan City, South Korea, or the Nakdong-Gang river drainage area, which are areas endemicity for C. sinensis infestation. Questionnaires about their residence, recent drug history related to parasite infestation, and consumption of raw freshwater fish were filled out by the visitors, who were divided into patients with C. sinensis infestation and a C. sinensis-free control group.A total of 51 adults were included in the patient group, of whom 43 were confirmed to have C. sinensis infestation by either the finding of eggs on stool microscopy (3...

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Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA

Cited by 78 publications

References 13 publications

Efficacy of Dot-ELISA using different antigens in detecting anti-schistosome antibodies among bovines in field conditions

Efficacy of Dot-ELISA using different antigens in detecting anti-schistosome antibodies among bovines in field conditions

Organ-specific antigens of Clonorchis sinensis

Performance of an Enzyme-Linked Immunosorbent Assay for Detection of Clonorchis sinensis Infestation in High- and Low-Risk Groups

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