Characterization of Oriental Cultivars of Grapevine Using a Reference Allele System of Microsatellite Data and Assignment Test

Abstract

Six Oriental cultivars of Vitis vinifera, which are native to Japan or China, as well as 2 cultivars of V. labrusca as references, were characterized by 6 microsatellite markers with coded reference alleles. One allele in Oriental cultivars and 3 in V. labrusca have not been reported for Occidental cultivars. Assignment analysis using these data and reported data with grouping of V. labrusca, Oriental cultivars, and vine-growing regions of Occidental cultivars showed that 4 out of 8 Oriental cultivars, but only 3 out of 162 Occidental cultivars, were assigned to Oriental cultivars. These assignments are in accordance with the genetic distances calculated from microsatellite data. These results show that Oriental cultivars have a certain degree of genetic difference from Occidental cultivars within the species V. vinifera.

Genetic Relationships of Chinese Grape Accessions to European and American Cultivars Assessed by Microsatellite Markers

“…Until now, only few papers have been published on the DnA based molecular markers analysis of chinese grape cultivars (15). investigation of genetic relationship is very import for germplasm conservation, evaluation and utilization for future grape breeding programs considering the present need of cultivar improvement.…”

“…in china, as many as 1500 accessions are conserved in national Grape Germplasm Repository of Zhengzhou, henan Province. however, only a limited number of oriental cultivars have been characterized (15).…”

Characterization of grape cultivars from China using microsatellite markers

“…Some alleles common in European cultivars were also observed in Chinese grape cultivars, but several new alleles were also found, such as alleles 235, 237 and 241 of primer VRZAG79 (Table 3). If we take into account only the oriental cultivars, more alleles were detected in this study than in the study of Goto-Yamamoto et al (2009).…”

“…They have been extensively used in grape for variety identification in collections, pedigree analysis, or genetic mapping (Karatas et al 2007;Vezzulli et al 2008;Laucou et al 2011;Moreno-Sanz et al 2011) and are very useful for distinguishing grape genotypes and determining genetic relationships among Vitis cultivars and species (Riahi et al 2012). Only a limited number of microsatellite data on oriental cultivars, particularly on Chinese cultivars, has been reported (Goto-Yamamoto et al 2009;Guo et al 2012a, b).…”

“…): pontica, orientalis, and occidentalis (Negrul 1938). Oriental cultivars commonly include Chinese and Japanese native cultivars, which were probably propagated along the Silk Road (Goto-Yamamoto et al 2009). Many old indigenous cultivars existed in China and some of them are still cultivated widely (Kong 2004).…”