Characterization of mutagenic compounds in heated orange juice by UV and mass spectra

Ekasari, Indriati; Fokkens, Roel H.; Bonestroo, M.H.; Schols, Henk A.; Nibbering, N. M. M.; Pilnik, W.

doi:10.1016/0308-8146(93)90079-u

Cited by 4 publications

(1 citation statement)

References 5 publications

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“…However, their results did not agree with the increase in mutagenicity reported by Ekasari, Jongen, and Pilnik (1986) in thermally treated juices. Ekasari et al (1993) worked with heated orange juice (93 C, 2 min) and, after submitting to Salmonella/mutagenicity assay, elucidated the molecular weight and chemical structure of the mutagenic agents. They consisted of several compounds with molecular weights of 162, 180, 254, 288, 342, 360, and 540 daltons.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of mutagenicity associated withEscherichia coliinactivation in UV-treated orange juice

Rodríguez

Oteiza²,

Giannuzzi

et al. 2016

Toxicological & Environmental Chemistry

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UV radiation is an alternative technology for the elimination of pathogenic micro-organisms in liquid food. The objectives of this work were to investigate mutagenesis using the Ames test in : (1) fresh squeezed orange juice submitted to UV radiation doses required to achieve 5 and 10 log 10 reductions of different strains of Escherichia coli serotype O157:H7 at two temperatures, and (2) commercial pasteurized orange juice submitted to thermal treatment. Two histidine-auxotrophic strains, Salmonella Typhimurium TA98 and TA97a, were used in the Ames test with and without metabolic activation. In commercial pasteurized orange juices, mutagenesis was not observed with S. Typhimurium TA98 strain with and without metabolic activation, but high mutagenic effect was observed using TA97a with metabolic activation. UV radiation did not affect the stability of ascorbic acid in aqueous solution. However, degradation of ascorbic acid was observed when UV treatment was performed on squeezed orange juice, following first order kinetics. UV treatment required to achieve 5 log 10 reductions (1.5 J/cm 2 ) did not show a mutagenic effect with and without metabolic activation using the tested strains, but a higher UV radiation dose necessary to produce 10 log 10 reductions (3.0 J/cm 2 ) showed mutagenicity with metabolic activation.

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Section: Discussionmentioning

confidence: 99%

Evaluation of mutagenicity associated withEscherichia coliinactivation in UV-treated orange juice

Rodríguez

Oteiza²,

Giannuzzi

et al. 2016

Toxicological & Environmental Chemistry

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Melanoidins Formed by Maillard Reaction in Food and Their Biological Activity

2012

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Involvement of Cdk Inhibitor p21(WIP1/CIP1) in G2/M Arrest of Human Myeloid Leukemia U937 Cells by N-Methyl-N'-Nitro-N-Nitrosoguanidine

Choi

2009

Journal of Life Science

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In this paper, to elucidate the further mechanisms of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced growth arrest, we investigated the effect of MNNG on cell cycle and proliferation in U937 cells, a p53-null human myeloid leukemia cell line. It was found that MNNG causes an arrest at the G2/M phase of the cell cycle and induces apoptosis, which is closely correlated to inhibition of cyclin B1 and cyclin-dependent kinase (Cdk) 2-associated kinase activities. MNNG treatment increased protein and mRNA levels of the Cdk inhibitor p21(WAF1/CIP1), and activated the reporter construct of a p21 promoter. By using p21 promoter deletion constructs, the MNNG-responsive element was mapped to a region between 113 and 61 relative to the transcription start site. These data indicate that in U937 cells MNNG can circumvent the loss of wild-type p53 function and induce critical downstream regulatory events leading to transcriptional activation of p21. Present results indicate that the p53-independent up-regulation of p21 by MNNG is likely responsible for the inhibition of cyclin/Cdk complex kinase activity rather than the down-regulation of cyclins and Cdks expression. These novel phenomena have not been previously described and provide important new insights into the possible biological effects of MNNG.

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Characterization of mutagenic compounds in heated orange juice by UV and mass spectra

Cited by 4 publications

References 5 publications

Evaluation of mutagenicity associated withEscherichia coliinactivation in UV-treated orange juice

Evaluation of mutagenicity associated withEscherichia coliinactivation in UV-treated orange juice

Melanoidins Formed by Maillard Reaction in Food and Their Biological Activity

Involvement of Cdk Inhibitor p21(WIP1/CIP1) in G2/M Arrest of Human Myeloid Leukemia U937 Cells by N-Methyl-N'-Nitro-N-Nitrosoguanidine

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