Characterization of microsatellites in the fungal plant pathogen Crinipellis perniciosa

Abstract

Microsatellite markers of Crinipellis perniciosa , with three and four repeats, were developed from sequence database and evaluated for their usefulness in detecting genetic polymorphism. Thirty-three primers produced unambiguous amplification products of 28 microsatellite-containing loci and 14 microsatellite-like polymorphic loci, with two to seven alleles at each locus. Three loci were useful to distinguish isolates from different biotypes and isolates from different countries. Amplification of the markers in the closely related fungi Moniliophthora roreri indicates that their usefulness in population's studies may go beyond the present study of the C. perniciosa and may have applications in population genetics of M. roreri .

Pathogenic variability of Moniliophthora perniciosa in three agroecological zones of the cacao region of Bahia, Brazil

“…Values of probability (F test) to reject the null hypothesis contrast between the means of progenies per variable and agroecological zone, and the mean of the inoculum mixture of each agroecological zone (Ploetz et al 2005, Gramacho et al 2007), among and within municipalities of the cacao region of Bahia, among parts of the same plant (fruits, flower cushions, vegetative shoots) and also among years of observation (Gramacho et al 2007, Patrocínio et al 2012. All these studies demonstrated genetic variability in the pathogen, without however addressing the difference in pathogenicity at the level of agroecological zones or municipalities in Bahia.…”

Development of novel microsatellites from Moniliophthora perniciosa, causal agent of the witches’ broom disease of Theobroma cacao

“…Understanding the structure of the genetic diversity of the pathogen would greatly assist cacao breeding programmes, and a set of international standard microsatellite loci is urgently required for fingerprinting M. perniciosa isolates. A few microsatellite loci have been proposed (Gramacho et al . 2007), but the number of available loci is still limited because of restricted polymorphism.…”

Avaliação da variabilidade de biotipos de Moniliophthora perniciosa

“…Na primeira, chamada de fase biotrófica, o fungo apresenta hifa primária (micélio biotrófico), relativamente larga (5 a 20 µm), sem grampos de conexão, que cresce intercelularmente sem causar a morte do tecido, formando a chamada vassoura-verde. Após dois a três meses o micélio passa por uma fase de transição (dicariotização), passando para a fase necrotrófica/saprofítica, com hifa secundária (micélio saprofítico) mais fina (1,5 a 3,0 µm), hialina, dicariótica, com grampos de conexão, que coloniza o tecido da planta intra e intercelularmente causando necrose e morte de tecidos infectados distante do local original de infecção, formando assim a vassoura-seca (ALBUQUERQUE et al, 2005;CALLE;COOK;FERNANDO, 1982;BASTOS, 1979;FRIAS;SCHIMIDT, 1991;KILARU;HASENSTEIN, 2005;MEINHARDT et al, 2006;SCARPARI et al, 2005;SHAW;VANDENBON, 2007;MATSUOKA, 1999 GRAMACHO et al, 2007;HEDGER, 1994a;PLOETZ et al, 2005;SCHMIDT, 1996;RINCONES et al, 2006).…”