Characterization of methicillin-resistant Staphylococcus aureus CC398 obtained from humans and animals on dairy farms

Feßler, Andrea T.; Riekerink, R. G. M. O.; Rothkamp, Anja; Kadlec, Kristina; Sampimon, O.C.; Lam, T.J.G.M.; Schwarz, Štefan

doi:10.1016/j.vetmic.2012.05.005

Cited by 79 publications

(66 citation statements)

References 33 publications

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“…The broader host range of CC398, affecting different animals and also humans, represents a potential threat to milkers and farm personnel, according to the significantly higher rates of CC398-MRSA nasal carriage by humans in contact with livestock (Cuny et al, 2013;Spohr et al, 2011). Accordingly, the results obtained by Feßler et al (2012) demonstrated a possible interspecies exchange of the same MRSA CC398 subtype between dairy cattle, humans, pigs and/or sheep, suggesting a clone diversification during colonization of different hosts on the same farm. Similarly, CC1 and CC97 seem to have a wide host range, as they have been isolated from bovine subclinical mastitis, pigs and also humans (Cuny et al, 2013).…”

Section: Discussionmentioning

confidence: 96%

“…CC97, t1730 MRSA co-existed with CC398-MSSA; CC1, t127 MRSA with CC8-MSSA and CC398, t899 MRSA with CC126-MSSA. CC398-MRSA has been widely reported in dairy animals (Feßler et al, 2012), and according to Price et al (2013), it originated in humans as MSSA; the jump to livestock was accompanied by the acquisition of methicillinresistance, probably as a consequence of widespread antibiotic use in food animal production. CC8-MSSA was recently reported as a frequent isolate from bovine mastitis in Western Switzerland (Sakwinska et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Methicillin-resistant Staphylococcus aureus (MRSA) is associated with low within-herd prevalence of intra-mammary infections in dairy cows: Genotyping of isolates

Luini

Cremonesi

Magro

et al. 2015

Veterinary Microbiology

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Methicillin-resistant Staphylococcus aureus (MRSA) is associated with low within-herd prevalence of intra-mammary infections in dairy cows: Genotyping of isolates

Luini

Cremonesi

Magro

et al. 2015

Veterinary Microbiology

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“…The European lineage (EMRSA-16) has been described to originate from sub-saharan Africa (Stegger et al, 2014) and has been reported in hospital and community acquired infections in Algeria (Abdulqader et al, 2014). Other lineages of human origin include CC1, CC5, CC8, CC22, CC30 and CC45 while MRSA lineage predominant in pigs and other food animals is CC398 (Witte et al, 2007;Feßler et al, 2012). Interspecies transmission of the strain CC398 (ST398) is a potential hazard and can be facilitated by frequent contact, environmental contamination and individual's immunity (Declercq et al, 2008).…”

Section: Epidemiology Of Mrsamentioning

confidence: 99%

Methicillin Resistant Staphylococcus aureus (MRSA): A Review

Grema¹,

Geidam²,

Gadzama³

et al. 2015

Adv. Anim. Vet. Sci.

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“…ivestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) strains mainly play a role as colonizers of foodproducing animals and humans who have occupational and otherwise close contact with these animals (1)(2)(3)(4)(5). However, they can also cause infections in humans (6) and animals (7,8).…”

mentioning

confidence: 99%

Identification of Multiresistance Gene cfr in Methicillin-Resistant Staphylococcus aureus from Pigs: Plasmid Location and Integration into a Staphylococcal Cassette Chromosome mec Complex

Wang

et al. 2015

Antimicrob Agents Chemother

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bThe multiresistance gene cfr was found in 8/231 porcine methicillin-resistant Staphylococcus aureus isolates. They were characterized by multilocus sequence typing, spa typing, dru typing, and staphylococcal cassette chromosome mec (SCCmec) typing as ST627-t002-dt12w-IVb, ST6-t304-dt12w-IVb, ST9-t899-dt12w-IVb, ST9-t899-dt12ae-IVb, or ST63-t899-dt12v-IVb. Different cfr gene regions were detected on plasmids of ca. 35 kb in seven isolates. For the first time, an ISEnfa4-cfr-IS256 fragment was found to be inserted upstream of the ccr genes in a chromosomal SCCmec IVb element of the remaining isolate. Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) strains mainly play a role as colonizers of foodproducing animals and humans who have occupational and otherwise close contact with these animals (1-5). However, they can also cause infections in humans (6) and animals (7,8). The important LA-MRSA clones are usually of the multilocus sequence type 398 (ST398) in the United States (1, 2) and European countries (9) and/or of ST9 in Asian countries (10), including China. LA-MRSA strains have acquired a number of novel and unusual antimicrobial resistance genes (11-15), including multiresistance genes that confer resistance to critically and highly important antimicrobial agents in human medicine (16). One such gene is the gene cfr, which confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A (17). Oxazolidinones are last resort antimicrobial agents for the control of serious infections caused by MRSA and vancomycin-resistant enterococci in humans. Although cfr-carrying MRSA strains have been reported in human medicine (18,19), cfr-carrying LA-MRSA strains of animal origin have been reported rarely (20,21).The 231 porcine MRSA isolates included in this study were collected in August 2012 and August 2013 from nasal swabs of healthy pigs at farms (n ϭ 7) and slaughterhouses (n ϭ 3) in the provinces of Henan (n ϭ 119) and Shandong (n ϭ 45) and in Shanghai (n ϭ 15) and from the lungs of diseased pigs at an animal hospital in the Guangdong province (n ϭ 52). All isolates were screened for the presence of cfr using previously described primers (22). Only eight (3.5%) of the MRSA isolates were positive for cfr. All cfr-carrying MRSA isolates had linezolid MICs of 4 mg/liter, which classifies them as borderline susceptible based on the current CLSI-approved breakpoints (23). cfr-positive staphylococci and enterococci with linezolid MICs of 4 mg/liter have been reported before (24, 25). The carriage rates for cfr differed geographically, with 5/15 (33.3%) cfr-positive isolates observed in Shanghai, followed by 2/52 (3.84%) in the Guangdong Province and 1/119 (0.84%) in the Henan Province. None of the isolates from Shandong contained cfr.The eight cfr-carrying isolates were subjected to staphylococcal chromosomal cassette mec (SCCmec) typing, multilocus sequence typing (MLST) (http://saureus.mlst.net), spa typing (http: //spaserver.ridom.de), dru typing (http://dr...

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Characterization of methicillin-resistant Staphylococcus aureus CC398 obtained from humans and animals on dairy farms

Cited by 79 publications

References 33 publications

Methicillin-resistant Staphylococcus aureus (MRSA) is associated with low within-herd prevalence of intra-mammary infections in dairy cows: Genotyping of isolates

Methicillin-resistant Staphylococcus aureus (MRSA) is associated with low within-herd prevalence of intra-mammary infections in dairy cows: Genotyping of isolates

Methicillin Resistant Staphylococcus aureus (MRSA): A Review

Identification of Multiresistance Gene cfr in Methicillin-Resistant Staphylococcus aureus from Pigs: Plasmid Location and Integration into a Staphylococcal Cassette Chromosome mec Complex

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