Characterization of metal chelate methacrylate monolithic disk for purification of polyclonal and monoclonal immunoglobulin G

Prasanna, Rajasekar R.; Vijayalakshmi, M.A.

doi:10.1016/j.chroma.2010.03.058

Cited by 36 publications

(24 citation statements)

References 26 publications

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“…The high selectivity and extension to a wide variety of ligand chemistry with CIM media have gained attention in the area of bio separation [35]. There are already several reports on purification of macromolecules like DNA, RNA, viruses like particles, IgM, IgG and enzymes using CIM ion exchange systems [35][36][37][38][39][40].…”

Section: Discussionmentioning

confidence: 99%

Purification and characterization of an extracellular uricase from a new isolate of Sphingobacterium thalpophilum (VITPCB5)

Ravichandran

Hemaasri

Cameotra

et al. 2015

Protein Expression and Purification

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Section: Discussionmentioning

confidence: 99%

Purification and characterization of an extracellular uricase from a new isolate of Sphingobacterium thalpophilum (VITPCB5)

Ravichandran

Hemaasri

Cameotra

et al. 2015

Protein Expression and Purification

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“…This could be due to the greater number of surface accessible histidine residues present in IgG, which interacted with the chelated metal ions. 36 Hale and Beidler described a histidine rich region in the third constant domain of heavy chain (CH3) of IgG. 37 Further molecular modeling studies performed by Todorova-Balvay et al revealed the accessibility of histidine residues located in the Fc region of IgG.…”

Section: Effect Of Metal Typementioning

confidence: 97%

Antibody purification from human plasma by metal‐chelated affinity membranes

Yavuz

Bereli

Armutçu

et al. 2011

J of Applied Polymer Sci

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The aim of this study is to investigate in detail the feasibility of poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester), PHEMAH membranes for purification of immunoglobulin G (IgG) from human plasma. PHEMAH membranes were prepared by photo-polymerization technique. Then, Zn 2þ , Ni 2þ , Co 2þ , and Cu 2þ ions were chelated directly on the PHEMAH membranes. Elemental analysis assay was performed to determine the nitrogen content and polymerized MAH was calculated as 168.5 lmol/g. The nonspecific IgG adsorption onto the plain PHEMA membranes was negligible (about 0.25 mg/mL). A remarkable increase in the IgG adsorption capacities were achieved from human plasma with PHEMAH membranes (up to 68.4 mg/mL).Further increase was observed with the metal-chelated PHEMAH membranes (up to 118 mg/mL). The metal-chelate affinity membranes allowed the one-step separation of IgG from human plasma. The binding range of metal ions for surface histidines from human plasma followed the order: Cu 2þ > Ni 2þ > Zn 2þ > Co 2þ . Adsorbed IgG was eluted using 250 mM EDTA with a purity of 94.1%. IgG molecules could be repeatedly adsorbed and eluted with the metal-chelated PHEMAH membranes without noticeable loss in their IgG adsorption capacity.

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“…CIM‐IDA ® discs with Cu 2+ , Ni 2+ and Zn 2+ were positively used in the purification of polyclonal and monoclonal IgG 66. The authors studied the influence of different buffer systems and flow rates over the binding and elution of IgG from the monolithic discs; a dynamic binding capacity up to 16 mg/mL was reached for the support CIM‐IDA‐Cu 2+ disc, whose value was in the same order than that obtained for CIM ® discs with specific ligands like Protein A and Protein G. Therefore, CIM ® discs with immobilized metals as pseudo‐affinity ligands could prove an interesting alternative for the purification of immunoglobulins.…”

Section: Special Applications Of Monolithic Columns In Acmentioning

confidence: 99%

Macroporous monolithic supports for affinity chromatography

Arrua

Igarzabal

2011

J of Separation Science

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In the early 1990s, three research groups simultaneously developed continuous macroporous rod-shaped polymeric systems to eliminate the problem of flow through the interparticle spaces generally presented by the chromatography columns that use particles as filler. The great advantage of those materials, forming a continuous phase rod, is to increase the mass transfer by convective transport, as the mobile phase is forced to go through all means of separation, in contrast to particulate media where the mobile phase flows through the interparticle spaces. Due to their special characteristics, the monolithic polymers are used as base-supports in different separation techniques, those chromatographic processes being the most important and, to a greater extent, those involving the separation of biomolecules as in the case of affinity chromatography. This mini-review reports the contributions of several groups to the development of macroporous monoliths and their modification by immobilization of specific ligands on the products for their application in affinity chromatography.

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Characterization of metal chelate methacrylate monolithic disk for purification of polyclonal and monoclonal immunoglobulin G

Cited by 36 publications

References 26 publications

Purification and characterization of an extracellular uricase from a new isolate of Sphingobacterium thalpophilum (VITPCB5)

Purification and characterization of an extracellular uricase from a new isolate of Sphingobacterium thalpophilum (VITPCB5)

Antibody purification from human plasma by metal‐chelated affinity membranes

Macroporous monolithic supports for affinity chromatography

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