Macroporous monolithic supports for affinity chromatography

Abstract

In the early 1990s, three research groups simultaneously developed continuous macroporous rod-shaped polymeric systems to eliminate the problem of flow through the interparticle spaces generally presented by the chromatography columns that use particles as filler. The great advantage of those materials, forming a continuous phase rod, is to increase the mass transfer by convective transport, as the mobile phase is forced to go through all means of separation, in contrast to particulate media where the mobile phase flows through the interparticle spaces. Due to their special characteristics, the monolithic polymers are used as base-supports in different separation techniques, those chromatographic processes being the most important and, to a greater extent, those involving the separation of biomolecules as in the case of affinity chromatography. This mini-review reports the contributions of several groups to the development of macroporous monoliths and their modification by immobilization of specific ligands on the products for their application in affinity chromatography.

Heparin removal from human plasma using molecular imprinted cryogels

“…Molecular imprinting is a sub field of biomimetic, which is very popular recently (Wulff 1995). Currently, MIPs have been used effectively in a wide range of fields from separation sciences, purification, sensors to drug delivery and extracorporeal therapy in consequence of their unique nature (Haginaka et al 1999, Lozinsky et al 2001, Wulff 2002, Piletsky et al 2006, Li and Li 2007, Haginaka 2008, Arrua and Alvarez Igarzabal 2011. Macroporous cryogels get great attention especially in biotechnology, biomedicine and pharmaceutics.…”

Recent advances in preparation and application of hybrid organic‐silica monolithic capillary columns

“…They therefore exhibit different advantages and disadvantages. A number of review articles have been published in the last decade describing progresses on their preparations, properties, and applications [12][13][14][15][16][17][18]. It can be easily found that the problems of both the swelling or shrinkage of organic monoliths in some solvents and the tedious fabrication procedures of the silica-based monoliths always encumber the researchers and limit the applications.…”

Oligoepoxide‐Based Monoliths: Synthesis and Application as Affinity Capillary Column for Enrichment of Immunoglobulin G

“…This includes the stability over a wide pH range considering strong changes in pH for elution, a low nonspecific binding, and the provision of functional groups for ligand immobilization 3. Over the last few years, supports based on organic monoliths have become more popular, which is reflected in publication of several reviews dealing with various applications of monolithic supports in affinity chromatography 4–12. Most organic monoliths such as commonly used support materials based on agarose are stable over a large pH range.…”