Purification and characterization of an extracellular uricase from a new isolate of Sphingobacterium thalpophilum (VITPCB5)

Ravichandran, Ramakrishnan; Hemaasri, S.; Cameotra, Swaranjit Singh; Jayaprakash, N.S.

doi:10.1016/j.pep.2015.06.017

Cited by 18 publications

(6 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3. Similarly lactose was found to be a good enhancer for uricase production by Sphingobacterium thalpophilum VITPCB5 [25]. While in case of various nitrogen sources tested at 1% level in the production medium, most of the organic sources significantly enhanced the uricase production whereas, nearly all inorganic sources moderately decreased the uricase production.…”

Section: Resultsmentioning

confidence: 90%

Statistical media optimization for the production of clinical uricase from Bacillus subtilis strain SP6

Pustake

Bhagwat

Dandge

2019

Heliyon

View full text Add to dashboard Cite

In this study, a potent uricase producing organism was isolated by a thorough screening and identified as Bacillus subtilis strain SP6 by using 16s rDNA sequencing. Response surface methodological optimization was employed for the enhanced production of uricase from newly isolated Bacillus subtilis strain SP6. In media optimization studies, Plackett Burman (PB) design was used for the selection of the critical media components; which were further optimized using central composite design (CCD). Lactose, soya peptone, uric acid and FeSO 4 .7H 2 O were found to be the critical factors influencing the enzyme production. Optimum uricase production with these factors was deduced using central composite design. Significant level of the factors were 12.2 g/L of lactose, 12.79 g/L of soya peptone, 2.55 g/L of uric acid and 0.00325 g/L FeSO 4 .7H 2 O. Use of statistical optimization upsurges uricase yield from 1.2 U/ml to 15.87 U/ml enhancing the overall production by 13.23 fold; which confirms that the model is effective for process optimization.

show abstract

Section: Resultsmentioning

confidence: 90%

Statistical media optimization for the production of clinical uricase from Bacillus subtilis strain SP6

Pustake

Bhagwat

Dandge

2019

Heliyon

View full text Add to dashboard Cite

show abstract

“…The endophytic characteristics of B. pumilus (Misaghi and Donndelinger 1990;Szilagyi-Zecchin et al 2014), P. aeruginosa (Gardner et al 1982;Jacobs et al 1985), E. aerogenes (Gardner et al 1982;Chen et al 2010;D'Alessandro et al 2014) and A. tumefaciens (Rashid et al 2012;Liaqat and Eltem 2016) were investigated earlier also. Previously S. thalpophilum was isolated from soil near the duck farm by Ravichandran et al (2015) and species of Sphingobacterium pakistanensis from rhizosphere of Vigna mungo by Ahmed et al (2014). In this study for the first time S. thalpophilum was isolated from W. somnifera as endophyte.…”

Section: Identification Of Isolates By 16s Rrna Sequencingmentioning

confidence: 86%

Indole acetic acid (IAA) producing endophytic bacteria on direct somatic embryogenesis and plant regeneration of Exacum travancoricum Bedd.

Raju¹,

Aslam²,

Thangadurai³

et al. 2020

Vegetos

View full text Add to dashboard Cite

Endophytic bacteria were isolated from Withania somnifera (L.) Dunal and their potentials were explored through the in vitro plant regeneration techniques. Initially, indole acetic acid (IAA), phosphate solubilization and siderophore producing isolates were screened out. These isolates were identified using molecular tools like 16s rRNA gene sequences namely Bacillus pumilus AS02, Sphingobacterium thalpophilum AS34, Pseudomonas aeruginosa AS36, Agrobacterium tumefaciens AS38 and Enterobacter aerogenes AS75. Further, the regeneration efficacy of IAA producing endophytic bacteria has been analysed on an endangered plant-Exacum travancoricum Bedd. The leaf and inter-node explants of E. travancoricum with or without bacterial infection were tested on Murashige and Skoog's (MS) medium encompassing 6-benzyladenine (BA; 2.0 mg L − 1 ) and l-tryptophan (0.2%; w/v). Somatic embryo induction was observed on both explants. The leaf explants infected with S. thalpophilum AS34, P. aeruginosa AS36 and E. aerogenes AS75 exhibited highest percentage of somatic embryo induction (93.3%) compared with the inter-node explants (86.6%). The explants without bacterial infection (control) and the presence of exogenous IAA exhibited lower level of response (88.8%, leaf and 75.5%, inter-node). Histological analysis revealed the appearance of globular embryogenic masses on epidermal, sub-epidermal regions of both explants. The development of torpedo, cotyledon stage and protoderm formation without the vascular connection from explants confirmed direct somatic embryogenesis. This was confirmed with scanning electron microscopy. Sub-culturing of cotyledon somatic embryos in MS liquid medium without growth regulators was performed to obtain well developed shoots with roots.

show abstract

“…aeruginosa was triggered by Ca 2+ , but inhibited by Co 2+ , Mn 2+ , Mg 2+ , Fe 2+ , Zn 2+ , and Cu 2+ in the growing media (Amirthanathan and Vijayakumar 2011), contrasting with the Ca 2+ , Mn 2+ , Mg 2+ , and Fe 2+ stimulation of the intracellular UOX in S. exfoliates (Aly et al 2013). While, the extracellular activity of UOX from S. thalpophilum was enhanced by Cu 2+ but partially inhibited by Ca 2+ , Fe 2+ Zn 2+ , and Ni 2+ (Ravichandran et al 2015). Details of the conversion of uric acid to HIU by the FAD-dependent UOX in K. pneumoniae were described by Hicks et al (2013).…”

Section: Overview Of the Main Enzymes And Genes Involved In The Bactementioning

confidence: 96%

Ureide metabolism in plant-associated bacteria: purine plant-bacteria interactive scenarios under nitrogen deficiency

Izaguirre‐Mayoral

Lazarovits²,

Baral

2018

Plant Soil

View full text Add to dashboard Cite

Background The erratic alterations in climate being experienced in agriculture, such as extended periods of drought or heavy rainfalls, are bringing increasing concerns about nitrogen (N) management. Even in highinput farming systems, unpredictable weather patterns can cause N deficiencies and result in nutrient losses that contribute to major pollution issues in groundwater, lakes, and even the oceans. Our present understanding of the beneficial interactions between N-deficientchallenged plants and plant-associated bacteria (PAB), mainly of the phyla Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria, is largely based on

show abstract

Purification and characterization of an extracellular uricase from a new isolate of Sphingobacterium thalpophilum (VITPCB5)

Cited by 18 publications

References 37 publications

Statistical media optimization for the production of clinical uricase from Bacillus subtilis strain SP6

Statistical media optimization for the production of clinical uricase from Bacillus subtilis strain SP6

Indole acetic acid (IAA) producing endophytic bacteria on direct somatic embryogenesis and plant regeneration of Exacum travancoricum Bedd.

Ureide metabolism in plant-associated bacteria: purine plant-bacteria interactive scenarios under nitrogen deficiency

Contact Info

Product

Resources

About