Characterization of Marinomonas algicida sp. nov., a novel algicidal marine bacterium isolated from seawater

Abstract

A novel Marinomonas-like, aerobic, Gram-reaction-negative, moderately halophilic, acidophilic, motile by a single polar flagellum, non-spore-forming, rod-shaped bacterium that showed algalytic activity, designated strain Yeongu 1-4, was isolated from surface seawater of Geoje Island in the South Sea, Republic of Korea. The strain was oxidase-negative and weakly positive for catalase. Growth of this bacterium was observed at temperatures from 4 to 42 °C, at salinities from 0 to 12 % and at pH from 4.5 to 9.0, and it was not able to degrade starch, gelatin, casein or Tween 80. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Yeongu 1-4 was related most closely to Marinomonas spartinae SMJ19 with similarity of 99.3 %. However, levels of DNA-DNA relatedness between strain Yeongu 1-4 and the most closely related species were lower than 70 %, confirming that they represent distinct genomic species. The genomic DNA G+C content of strain Yeongu 1-4 was 44.2 mol%. The organism used Q-8 as the predominant respiratory quinone, and C16 : 1ω7c, C18 : 1ω7c and C16 : 0 as major cellular fatty acids. Based on data from this polyphasic taxonomic study, strain Yeongu 1-4 belongs to a novel species of the genus Marinomonas, within the family Oceanospirillaceae, for which the name Marinomonas algicida is proposed. The type strain is Yeongu 1-4 (=KEMB 9005-327=MCCC 1K00609).

Microbial Community Dynamics and Assembly Follow Trajectories of an Early-Spring Diatom Bloom in a Semienclosed Bay

“…Nine OTUs with negative correlations with S. costatum belong to Oceanospirillaceae and Flavobacteriaceae. Members of Oceanospirillaceae and Flavobacteriaceae have long been reported to have algicidal activity (35,36), which may inhibit the growth of S. costatum. Therefore, our results indicate that some dominant bacterial members may change the course of diatom blooms, according to the interactions between bacteria and diatoms.…”

Marinomonas transparens sp. nov. and Marinomonas sargassi sp. nov., isolated from marine alga

“…As more species were included, the genus description was later further emended to accommodate bacteria with Gram-stain-negative, Na + -required, helical, curved or straight rods that are motile by means of polar flagella at one or both poles of the cells [3]. The genus Marinomonas comprises 38 species with validly published names (https://lpsn.dsmz.de/genus/marinomonas) at the time of writing, all from marine environments [4][5][6][7][8][9][10][11] and many of them are found associated with macroalgae [3,12] . Here, two Marinomonas-like bacterial strains, designated C1424 T and C2222 T , were isolated from a marine brown alga (Sargassum natans (L.) Gaillon) of a Phaeophyta sample collected from the sea shore of Yantai, PR China (121.46° E 37.47° N), in December 2017.…”

Marinomonas lutimaris sp. nov., isolated from a tidal flat sediment of the East China Sea

“…de/genus/marinomonas). Most species have been isolated from seawater [4][5][6][7][8][9][10][11][12] and marine sediments [13][14][15][16], as well as corals [17], seagrass [18], macroalgae [19,20] and reared clams (Ruditapes decussatus) [21]. The genus Marinomonas is widespread in diverse marine environments, including Antarctica [6,10], the Mariana and Yap Trenches [9,15], the Pacific Ocean [15], the Black Sea [22], the Arctic Ocean [6,10] and mangrove sediment [16].…”