Characterization of Magnesium Orotate‐Loaded Chitosan Polymer Nanoparticles for a Drug Delivery System

Abstract

The drug release properties of magnesium orotate (MgOr) encapsulated in the chitosan (CS) cavity and the complexation behavior between MgOr and CS were investigated. The MgOr-loaded CS nanoparticles (MgOrCSNPs) were characterized by differential scanning calorimetry, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and scanning electron microscopy with energy-dispersive X-ray spectroscopy. MgOr was successfully encapsulated into the CS cavity. Results with 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide indicated that MgOrCSNPs retained their cytotoxic activity against the liver cancer cell line (HepG2) and breast cancer cell line (MCF-7), and low toxicity against the human cell line (3T3) and human retinal epithelial cell line .

Compatibility Study Between Magnesium Orotate and Various Excipients in Their Physical Mixtures

“…Analysing the DTA curve of MO-C it can be observed the appearance of a new endothermic peak at 332°C attributed to the decomposition of C, which is in concordance with previous results in literature [8].…”

“…Being organic compounds, the theoretical mass loss of excipients should be 100%, however in our case the mass loss in the case of organic excipients (32.37% CS, 43.1% C, 19.9 CC) is much smaller since the experiments were performed in nitrogen. Literature survey revealed that the decomposition of CS (same as C) take place in one stage, in the temperature range 300 -400°C [8,22], which confirm our results. L and S, in combination with MO, showed significant thermal changes as displayed in Figure 5a and Figure 5b.…”

Formulation, Characterization and Biological Activity Screening of Sodium Alginate-Gum Arabic Nanoparticles Loaded with Curcumin

“…However, among the many obstacles facing these therapeutic agents is their limited absorption and retention by cancer cells which also lead to toxic effects in normal tissue cells [1][2][3][4]. In this quest, the development of nanoparticle formulations of different therapeutic agents served in providing a better delivery tool of chemotherapies with better dosage and targeting precision to the tumor site [5][6][7][8][9].…”