Characterization of Lipoamino-Acids as O-Amino-Acid Esters of Phosphatidyl-Glycerol

Macfarlane, Marjorie G.

doi:10.1038/196136a0

Cited by 163 publications

(73 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To prepare phosphatidic acid a sample of pure phosphatidyl choline was hydrolysed with the phospholipase D of cabbage by the method of Kates (1954). Phospholipase A and D and sphingomyelin were obtained from Koch-Light Laboratories Ltd. Diphosphatidyl glycerol (cardiolipin) was prepared from pig heart by the technique of MacFarlane (1961). Pure fatty acids and their methyl esters were obtained from Calbiochem Inc., New York.…”

Section: Inhibition Of C Welchii By Lipids 25mentioning

confidence: 99%

The Inhibition of the Growth of Clostridium welchii by Lipids Isolated from the Contents of the Small Intestine of the Pig

Fuller¹,

Moore²

1967

Journal of General Microbiology

View full text Add to dashboard Cite

Section: Inhibition Of C Welchii By Lipids 25mentioning

confidence: 99%

The Inhibition of the Growth of Clostridium welchii by Lipids Isolated from the Contents of the Small Intestine of the Pig

Fuller¹,

Moore²

1967

Journal of General Microbiology

View full text Add to dashboard Cite

“…One important bacterial response to such compounds is the aminoacylation of PG resulting in a reduction of the overall net negative charge of the membrane. The resulting aminoacylphosphatidylglycerol (aaPG) molecules can either be zwitterionic (A-PG) or alternatively they carry an overall positive net charge, as is the case for lysyl-phosphatidylglycerol (L-PG) (20,34,43,56,75,82). The resulting charge characteristics of the bacterial envelope were also proposed to have a profound impact on biophysical properties like membrane fluidity and lipid head group interaction (72,76,89).…”

mentioning

confidence: 99%

Resistance Phenotypes Mediated by Aminoacyl-Phosphatidylglycerol Synthases

et al. 2012

View full text Add to dashboard Cite

The specific aminoacylation of the phospholipid phosphatidylglycerol (PG) with alanine or with lysine catalyzed by aminoacylphosphatidylglycerol synthases (aaPGS) was shown to render various organisms less susceptible to antibacterial agents. This study makes use of Pseudomonas aeruginosa chimeric mutant strains producing lysyl-phosphatidylglycerol (L-PG) instead of the naturally occurring alanyl-phosphatidylglycerol (A-PG) to study the resulting impact on bacterial resistance. Consequences of such artificial phospholipid composition were studied in the presence of an overall of seven antimicrobials (␤-lactams, a lipopeptide antibiotic, cationic antimicrobial peptides [CAMPs]) to quantitatively assess the effect of A-PG substitution (with L-PG, L-PG and A-PG, increased A-PG levels). For the employed Gram-negative P. aeruginosa model system, an exclusive charge repulsion mechanism does not explain the attenuated antimicrobial susceptibility due to PG modification. Additionally, the specificity of nine orthologous aaPGS enzymes was experimentally determined. The newly characterized protein sequences allowed for the establishment of a significant group of A-PG synthase sequences which were bioinformatically compared to the related group of L-PG synthesizing enzymes. The analysis revealed a diverse origin for the evolution of A-PG and L-PG synthases, as the specificity of an individual enzyme is not reflected in terms of a characteristic sequence motif. This finding is relevant for future development of potential aaPGS inhibitors.

show abstract

“…3 Gaby, Wolin, and Zajac 3 7 and Hunter and James5 have suggested :hat these complexes are involved in amono acid transport processea.…”

Section: Discussionmentioning

confidence: 99%

Lipids of Sarcina Lutea. 3. Composition of the Complex Lipids

Huston¹,

Albro²,

Grindey³

1964

View full text Add to dashboard Cite

The complex lipids of a strain of Saroina lutea were isolated and separated into fractions on DFAE cellulose acetate and silicic acid columns.These fractions were monitored in several thin-layer cbromatography aystems.The various lipid types were characterized by their behavior in thin-layer systems and by an analysis of their hydrolysis products.The fatty acid composition of the column fractions was determined by gas-liquid chromeatography.Thirteen components were separated by thin-layer chromatography and characterised. Ethanolamine is the most commonly reported nitrogen-containing component; chloline is found in some species. Recent reports have also described the isolation of lipoamino acid complexes in the complex lipids of bacteria. -6We have reported 7 that Sarcina lutea contains a complex mixture of highly polar lipids that comprise approximately 23% of the total extractable lipid. The determination of the composition of this polar fraction is reported in this manuscript. II. MATERIALSThe preciseness of the experimental procedures and .h;! small quantities of lipids under investigation required extreme purity and reliability in the reagents employed. For these reasons the following materials were obtained from the sources shown: I) Whatman diethylaminoethyl (DEAE) celluloseScientifica, Clifton, New Jersey.2) Silicic acid, analytical:reagent grade -Mallinckrodt Chemical Works, New York, New York.3) Inorganic reggents, solvents, and Hyflo Super Cel -Fisher Scientific Company, Silver Spring, Maryland.4) Synthetic phosphatidyl ethanolamine, phosphatidyl serine, and phosphatidyl choline (all dipalmitoyl) A-grpde -Calbiochem,

show abstract

Characterization of Lipoamino-Acids as O-Amino-Acid Esters of Phosphatidyl-Glycerol

Cited by 163 publications

References 26 publications

The Inhibition of the Growth of Clostridium welchii by Lipids Isolated from the Contents of the Small Intestine of the Pig

The Inhibition of the Growth of Clostridium welchii by Lipids Isolated from the Contents of the Small Intestine of the Pig

Resistance Phenotypes Mediated by Aminoacyl-Phosphatidylglycerol Synthases

Lipids of Sarcina Lutea. 3. Composition of the Complex Lipids

Contact Info

Product

Resources

About