Characterization of isolates of the entomopathogenic fungus Metarhizium anisopliae by RAPD-PCR

Leal, Soraya C. M.; Bertioli, David J.; Butt, Tariq M.; Peberdy, John F.

doi:10.1016/s0953-7562(09)80436-x

Cited by 64 publications

(38 citation statements)

References 13 publications

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“…A lot of studies indicate the crucial importance of the geographical origin of isolates (St. Leger et al, 1992;Cobb & Clarkson, 1993;Leal et al, 1994;Leal et al, 1997) and habitat type (Bidochka et al, 2001). Studies of Leal et al (1994;1997) showed that among 40 isolates of M. anisopliae, strains from the same country were more similar in the genetic structure than those from different countries despite the same host. Interesting observations to support a relationship between the genetic structure of M. anisopliae isolates and habitat type (agricultural and forested areas) along with abiotic factors (temperature and exposure to UV radiation) provided Bidochka et al (2001).…”

Section: Intraspecific Variation Of Entomopathogenic Fungimentioning

confidence: 99%

Entomopathogenic Fungi as an Important Natural Regulator of Insect Outbreaks in Forests (Review)

Augustyniuk-Kram¹,

Kram²

2012

Forest Ecosystems - More Than Just Trees

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The common idea for many people is that forests are just a collection of trees. However, they are much more than that. They are a complex, functional system of interacting and often interdependent biological, physical, and chemical components, the biological part of which has evolved to perpetuate itself. This complexity produces combinations of climate, soils, trees and plant species unique to each site, resulting in hundreds of different forest types around the world. Logically, trees are an important component for the research in forest ecosystems, but the wide variety of other life forms and abiotic components in most forests means that other elements, such as wildlife or soil nutrients, should also be the focal point in ecological studies and management plans to be carried out in forest ecosystems. In this book, the readers can find the latest research related to forest ecosystems but with a different twist. The research described here is not just on trees and is focused on the other components, structures and functions that are usually overshadowed by the focus on trees, but are equally important to maintain the diversity, function and services provided by forests. The first section of this book explores the structure and biodiversity of forest ecosystems, whereas the second section reviews the research done on ecosystem structure and functioning. The third and last section explores the issues related to forest management as an ecosystem-level activity, all of them from the perspective of the �other� parts of a forest.

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Section: Intraspecific Variation Of Entomopathogenic Fungimentioning

confidence: 99%

Entomopathogenic Fungi as an Important Natural Regulator of Insect Outbreaks in Forests (Review)

Augustyniuk-Kram¹,

Kram²

2012

Forest Ecosystems - More Than Just Trees

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“…Other well known, but less specific mycoinsecticides used in repeated control of various insects are the hyphomycetes Beauveria bassiana and Metarhizium anisopliae (for further details on fungal control of insect pests see Shah & Pell 2003). Genetic markers [microsatellites, randomly amplified polymorphic DNA (RAPD) markers, restriction fragment length polymorphism (RFLP); for more detailed explanations see Chapter 8 of this book] have been defined to monitor Beauveria and Metarhizium strains in the field (Leal et al 1994, Maurer et al 1997, Bidochka et al 2001, Enkerli et al 2001.…”

Section: Biological Control Of Harmful Insectsmentioning

confidence: 99%

Wood production, wood technology, and biotechnological impacts

Kües¹

2007

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“…To determine its effectuality, host specificity, survival and partial temporal distribution within the field, proper markers are required for the individual strains (Leal et al, 1994). DNA markers can give more précised genetic information compared to isozymes and they are not changed by environmental conditions (Tigano et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic diversity among different isolates of Beauveria bassiana by RAPD-PCR

Prabhukarthikeyan

Keerthana

Archana

et al. 2017

Journal of Biological Control

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ABSTRACT:The genetic diversity of Beauveria bassiana isolated from the different ecosystem of Tamil Nadu was analyzed. Totally fifteen isolates were used in this study. The genetic variability and relationship among 15 isolates were analyzed using 15 Random Amplified Polymorphic DNA (RAPD) markers. Among 15 primers used, six primers viz., OPA02, OPF01, OPX07, OPA03, OPZ19 and OPG19 showed 100 percent polymorphism. The relationships among the isolates were examined and represented as dendrogram by using UP-GMA clusters. Results revealed that similarity coefficients of B. bassiana isolates based on RAPD markers ranged from 42.00 to 86.00 percent. The maximum of 85 percent similarity was observed between the isolates B23 and B24. All isolates have significantly different from one another. Overall we concluded that RAPD was found to be better in assessing genetic diversity among B. bassiana isolates.

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Characterization of isolates of the entomopathogenic fungus Metarhizium anisopliae by RAPD-PCR

Cited by 64 publications

References 13 publications

Entomopathogenic Fungi as an Important Natural Regulator of Insect Outbreaks in Forests (Review)

Entomopathogenic Fungi as an Important Natural Regulator of Insect Outbreaks in Forests (Review)

Wood production, wood technology, and biotechnological impacts

Analysis of genetic diversity among different isolates of Beauveria bassiana by RAPD-PCR

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