Analysis of genetic diversity among different isolates of Beauveria bassiana by RAPD-PCR

Abstract

ABSTRACT:The genetic diversity of Beauveria bassiana isolated from the different ecosystem of Tamil Nadu was analyzed. Totally fifteen isolates were used in this study. The genetic variability and relationship among 15 isolates were analyzed using 15 Random Amplified Polymorphic DNA (RAPD) markers. Among 15 primers used, six primers viz., OPA02, OPF01, OPX07, OPA03, OPZ19 and OPG19 showed 100 percent polymorphism. The relationships among the isolates were examined and represented as dendrogram by using UP-GMA clusters. Results revealed that similarity coefficients of B. bassiana isolates based on RAPD markers ranged from 42.00 to 86.00 percent. The maximum of 85 percent similarity was observed between the isolates B23 and B24. All isolates have significantly different from one another. Overall we concluded that RAPD was found to be better in assessing genetic diversity among B. bassiana isolates.

Molecular characterization of new isolates of the entomopathogenic fungus Beauveria bassiana and their efficacy against the tobacco caterpillar, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)

“…High genetic divergence of BbM1 from the new isolates from Punjab soils in this study may be due to the reason that the former was originally isolated in 1988 from IISR, Lucknow, a distantly located geographic region of India (Varma et al 1988), and faced repeated sub-culturing at MTCC, Institute of Microbial Technology, Chandigarh. Various workers have effectively used the RAPD to assess the genetic diversity of different isolates of B. bassiana (Kaur and Padmaja 2008;Imoulan et al 2016;Prabhukarthikeyan et al 2017). The studies reported the high genetic diversity among local isolates of B. bassiana, which supported the diversity of maximum 42% between standard MTCC2028 and the obtained new isolates.…”

Beauveria bassiana: Biopestisida Ramah Lingkungan dan Efektif untuk Mengendalikan Hama dan Penyakit Tanaman

“…Salah satu faktor yang mempengaruhi efikasi B. bassiana yaitu virulensi cendawan akibat terjadinya keragaman antarisolat Prabhukarthikeyan et al 2017;Dhar et al 2019;Boston et al 2020). Sementara itu, keragaman antarisolat berpengaruh langsung terhadap produksi jenis enzim dan toksin yang dihasilkan sebagai senjata utama dalam membunuh serangga dan patogen sasaran (Khan et al 2016;Saleem dan Ibrahim 2019;da Silva et al 2020;Sayed et al 2021).…”

“…Cendawan B. bassiana selain sebagai agens pengendalian hama juga bersifat endofit yang hidup di dalam jaringan sehingga dapat melindungi tanaman dari berbagai jenis penyakit penting (Bonnie et al 2010;Castillo-Lopez dan Sword 2015;Greenfield et al 2016;Prabhukarthikeyan et al 2017;Jaber dan Ownley 2018;Mwanburi 2021). Berbagai jenis penyakit tanaman yang dapat dihambat perkembangannya oleh cendawan B. bassiana yaitu penyakit tular tanah (soil born disease) antara lain: Botrytis cinerea, Verticillium dahliae, Phytophthora megapserma, dan beberapa penyakit tular tanah lainnya pada berbagai jenis tanaman pangan, perkebunan maupun hortikultura (Akello et al 2007;Ownley et al 2008;Powell et al 2009;Tefera dan Vidal 2009;Punja et al 2016;Lozano-Tovar et al 2017;Saragih et al 2019;Barra-Bucarei et al 2020).…”