Characterization of immobilization methods of antiviral antibodies in serum for electrochemical biosensors

Huy, Tran Quang; Hạnh, Nguyễn Thị Hồng; Chung, Pham Van; Anh, Đặng Đức; Nga, Phan Thi; Tuan, Mai Anh

doi:10.1016/j.apsusc.2011.03.051

Cited by 44 publications

(40 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The samples with PrA/GA/APTMS surfaces were changed from one tube to another, from PBS solution to this diluted serum, and allowed to react them for 20 h at 4 °C. Then, the samples were immersed in 2 mg/mL of bovine serum albumin (BSA) in PBS (pH 7.4) solution for 30 min at about 4 °C In this step, the BSA/PBS solution acts as a blocking agent for the terminal groups that did not bind to any antibody [8]. All samples were rinsed with PBS (pH 7.0) solution inside tubes to remove weak interactions.…”

Section: E) Immobilization Of Anti-e Coli Polyclonal Antibodiesmentioning

confidence: 99%

“…The IMA and the a-SiC:H thin films on silicon substrates (samples) were immersed in a solution of potassium hydroxide and methanol (45% KOH/MeOH, 1:1) for 15 min, making hydrophilic the surfaces [8]. They were then rinsed in deionized (DI) water and dried under a stream of nitrogen gas.…”

Section: Biofunctionalization Process A) Hydroxylationmentioning

confidence: 99%

“…Furthermore, the development of a biofunctionalization process for the surface of an amorphous network which in turn increases the sensitivity of a biosensor is not an easy task. The surface of an amorphous network is inhomogeneous, making difficult to obtain homogeneous self-assembled layers and oriented away from the surface [7,8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biofunctionalization Process of a-SiC:H Surfaces Applied to an Interdigitated Microelectrode Array to Detect Enterotoxigenic Escherichia coli

Herrera-Celis

Reyes-Betanzo

Orduña-Díaz

et al. 2017

VII Latin American Congress on Biomedical Engineering CLAIB 2016, Bucaramanga, Santander, Colombia, October 26th -28th, 2016

View full text Add to dashboard Cite

Abstract-A biofunctionalization process of a-SiC:H surfaces has been applied to an interdigitated microelectrode array (IMA) whose microelectrodes are covered by this material. The biofunctionalization process has been monitored stage by stage using Fourier transform infrared spectroscopy (FTIR), while its effects on the electrical behavior of the IMA were recorded in electrical impedance spectra. The process involves hydroxylation, silanization, generation of aldehyde groups, binding via protein A, immobilization of anti-Escherichia coli polyclonal antibodies, entrapping and detection of enterotoxigenic Escherichia coli (ETEC) in Luria-Bertani medium. The FTIR spectra confirm the success of the process. Regarding the performance of the IMA, although the detection of ETEC is successful and its percentage change in impedance reaches a value of 133.37% to 10 7 CFU/mL, some considerations may be taken into account to improve the sensitivity of the IMA by mean of the optimization of both the IMA design and the biofunctionalization process.Keywords-Hydrogenated amorphous silicon carbide, biofunctionalization process, enterotoxigenic Escherichia coli, Fourier transform infrared spectroscopy, electrical impedance spectroscopy.

show abstract

Section: E) Immobilization Of Anti-e Coli Polyclonal Antibodiesmentioning

confidence: 99%

Section: Biofunctionalization Process A) Hydroxylationmentioning

confidence: 99%

See 1 more Smart Citation

Biofunctionalization Process of a-SiC:H Surfaces Applied to an Interdigitated Microelectrode Array to Detect Enterotoxigenic Escherichia coli

Herrera-Celis

Reyes-Betanzo

Orduña-Díaz

et al. 2017

VII Latin American Congress on Biomedical Engineering CLAIB 2016, Bucaramanga, Santander, Colombia, October 26th -28th, 2016

View full text Add to dashboard Cite

show abstract

“…The interdigitated electrode was silanized by APTES [8], and incubated with the GNPs/PrA complex (diluted 1 : 60 in 0.5% BSA/PBS) for 45 min. Then, it was washed carefully in 0.2% BSA/PBS and subsequently incubated in 1 mg ml…”

Section: Scanning Electron and Fluorescence Microscopiesmentioning

confidence: 99%

“…3 (2012) 025013 Q H Tran et al micro-electrodes, leading to amplification of the signal of the electrochemical sensor with or without electron transfer mediators [6,7]. Furthermore, protein A (PrA) molecules are very heat-stable and bind well to the fragment crystallizable (Fc) region of the immunoglobulin molecules without disturbing antigen binding sites [1,8], leading to the improvement of the possibility for detecting viral antigens in solution. Hence, the combination of gold nanoparticles and PrA could be ideal for signal amplification of electrochemical immunosensors in direct detection of virus from clinical samples.…”

Section: Introductionmentioning

confidence: 99%

Towards the use of protein A-tagged gold nanoparticles for signal amplification of electrochemical immunosensors in virus detection

Huy

Tuan

Nguyen

et al. 2012

Adv. Nat. Sci: Nanosci. Nanotechnol.

View full text Add to dashboard Cite

In this paper we represent a study on the potential use of protein A-tagged gold nanoparticles applied for signal amplification of electrochemical immunosensors. Gold nanoparticles (GNPs) were synthesized by the chemical reduction of tetrachloroauric (III) acid trihydrate using sodium ascorbate, and then tagged with protein A (PrA) via ultracentrifugation. UV-Vis spectroscopy and transmission electron microscopy were used to verify the characteristics of formed GNPs/PrA complex. The analyzed results indicate that GNPs were found spherically, homogeneously, and with an average diameter of about 10 nm. Immunoelectron microscopy was then used to investigate the bioactivity of the GNPs/PrA complex in solution by the effective binding of GNPs to viral particles. Scanning electron and fluorescence microscopies were also used to investigate the distribution and the bioactivity of the GNPs/PrA complex on the surface of the interdigitated sensor. Consequently, this study provided some assumptions of the potential application of protein A-tagged gold nanoparticles for signal amplification of electrochemical immunosensors in virus detection from clinical samples.

show abstract

Lipoprotein(a) determination in human serum using a nitrilotriacetic acid derivative immunosensing scaffold on disposable electrodes

et al. 2014

View full text Add to dashboard Cite

A novel strategy for the construction of a disposable integrated amperometric immunosensor for the sensitive and rapid determination of lipoprotein(a) (Lp(a)), an important predictor of cardiovascular disease risk, in human serum is reported. The approach uses a sandwich format involving the covalent immobilization of selective capture antibodies (antiLp(a)) on the surface of N-[Nα,Nα-bis(carboxymethyl)-lysine]-12-mercaptododecanamide (HS-NTA)-modified screen-printed carbon electrodes (SPCEs). After a blocking step with skimmed milk, the modified antiLp(a)-SPCEs were incubated with a mixture solution containing the target analyte and a fixed concentration of a specific biotinylated antibody (biotin-antiLp(a)) and a streptavidin-horseradish peroxidase (HRP) (Strep-HRP) conjugate. The amperometric responses of the resulting immunosensor at -0.10 V (vs an Ag pseudo-reference electrode), upon addition of 3,3',5,5'-tetramethylbenzidine (TMB) as electron transfer mediator and H2O2 as the enzyme substrate, were used to monitor the extent of the immunoreactions. The developed methodology exhibited a wide range of linearity between 0.02 and 10 μg mL(-1), a low detection limit (LOD) of 8 ng mL(-1), and a great selectivity against other serum components. The usefulness of the Lp(a) immunosensor was demonstrated by analyzing spiked serum samples as well as a reference serum containing a certified Lp(a) content.

show abstract

Characterization of immobilization methods of antiviral antibodies in serum for electrochemical biosensors

Cited by 44 publications

References 30 publications

Biofunctionalization Process of a-SiC:H Surfaces Applied to an Interdigitated Microelectrode Array to Detect Enterotoxigenic Escherichia coli

Biofunctionalization Process of a-SiC:H Surfaces Applied to an Interdigitated Microelectrode Array to Detect Enterotoxigenic Escherichia coli

Towards the use of protein A-tagged gold nanoparticles for signal amplification of electrochemical immunosensors in virus detection

Lipoprotein(a) determination in human serum using a nitrilotriacetic acid derivative immunosensing scaffold on disposable electrodes

Contact Info

Product

Resources

About