Characterization ofYersinia enterocoliticastrains isolated from cattle, sheep and pigs in the United Kingdom

Abstract

SUMMARYThe properties of 48 cultures identified asYersiniaenterocoliticaorY. enterocoliticalike organisms, includingY. frederiksenii,Y. intermediaandY. kristensenii, were examined. Of these, 39 were isolated from flieces of apparently healthy pigs, five from healthy cattle, one from an aborted bovine fetus, one from an aborted lamb and one from a lamb suffering from acute enteritis.Most isolates from healthy animals were ofY. enterocoliticabiotype 1 orY. intermediaand belonged to O serogroups not usually associated with disease in man or animals.The isolates from abortion or enteritis cases were ofY. enterocoliticabiotypes 3, 4 and 5 and belonged to the pathogenic serogroups 0:5b and 0:2a, 2b, 3. No organisms of serogroup 0:9 were found.

Analysis of the Presence ofail,ystAandystBGenes inYersinia enterocoliticaStrains Isolated from Aborting Sows and Aborted Fetuses

“…However, some of these strains caused stomach and intestinal diseases in humans (Ratnam et al., 1982) and the disease course was identical to that evoked by classic virulent biotypes (Robins‐Browne et al., 1993; Burnens et al., 1996; Bottone, 1997; Grant et al., 1998). Yersinia enterocolitica biotype 1A serotypes O:5 and O:6,30 also caused abortions in sheep and cattle (Brewer and Corbel, 1983; Das et al., 1986; Corbel et al., 1990, 1992). However, the pathogenic mechanisms during the course of diseases caused by biotype 1A have not been clarified and there are no commonly established virulence markers for this biotype (Ramamurthy et al., 1997; Grant et al., 1998; Tennant et al., 2005).…”

“…So far, little is known about isolates of these bacteria originating from other sources. Only scarce reports describe strains isolated from cases of bovine and ovine abortion caused by Y. enterocolitica (Brewer and Corbel, 1983; Das et al., 1986; Corbel et al., 1990, 1992). There is no information on Y. enterocolitica isolates from aborting sows.…”

Prevalence of Anti‐Yersinia Outer Protein Antibodies in Goats in Lower Saxony

“…It can be speculated that a similar clinical picture as in sheep may be seen and that ‘ovine’ strains may also cause caprine infection, i.e. the pathogenic Y. enterocolitica serotype O:2a, 2b, 3b, c biotype 5, serotypes O:3 and O:9 as well as Y. pseudotuberculosis serotypes I–III (Brewer and Corbel, 1983; Ludes and Weiss, 1984; Wuthe and Aleksic, 1997; Hartung, 2000). New molecular typing methods may provide then a deeper understanding of epidemic cycles (Wojciech et al., 2004).…”

“…The whiskers extend to the most extreme data point <1.5 times the interquartile range from the box. Mean and outliers Go¨ttingen (115 (7) 57.14 (Brewer and Corbel, 1983;Ludes and Weiss, 1984;Wuthe and Aleksic, 1997;Hartung, 2000). New molecular typing methods may provide then a deeper understanding of epidemic cycles (Wojciech et al, 2004).…”

Gene polymorphism analysis of Yersinia enterocoliticaouter membrane protein A and putative outer membrane protein A family protein

“…Biotype 1A strains lack pYV plasmid and chromosomal virulence genes, and generally regarded as avirulent [ 1 ]. However, few studies have confirmed biotype 1A strains were related to outbreaks of nosocomial infections and foodborne diarrhea [ 20 , 21 ]; and some early studies found that biotype 1A strains could cause abortion in goats and cattle [ 22 – 25 ]. Grant et al [ 26 , 27 ] showed biotype 1A strains invaded epithelial cells and resisted the killing effect of macrophage.…”