Gene polymorphism analysis of Yersinia enterocoliticaouter membrane protein A and putative outer membrane protein A family protein

Outer membrane protein A (OmpA) is one of the most abundant outer membrane proteins of Gram-negative bacteria and is known to have patterns of sequence variations at certain amino acids—allelic variation—in Escherichia coli. Here we subjected seven exemplar OmpA alleles expressed in a K-12 (MG1655) ΔompA background to further characterization. These alleles were observed to significantly impact cell surface charge (zeta potential), cell surface hydrophobicity, biofilm formation, sensitivity to killing by neutrophil elastase, and specific growth rate at 42°C and in the presence of acetate, demonstrating that OmpA is an attractive target for engineering cell surface properties and industrial phenotypes. It was also observed that cell surface charge and biofilm formation both significantly correlate with cell surface hydrophobicity, a cell property that is increasingly intriguing for bioproduction. While there was poor alignment between the observed experimental values relative to the known sequence variation, differences in hydrophobicity and biofilm formation did correspond to the identity of residue 203 (N vs T), located within the proposed dimerization domain. The relative abundance of the (I, δ) allele was increased in extraintestinal pathogenic E. coli (ExPEC) isolates relative to environmental isolates, with a corresponding decrease in (I, α) alleles in ExPEC relative to environmental isolates. The (I, α) and (I, δ) alleles differ at positions 203 and 251. Variations in distribution were also observed among ExPEC types and phylotypes. Thus, OmpA allelic variation and its influence on OmpA function warrant further investigation.

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“…There are reports of allelic variation of OmpA for both Y . enterocolitica [ 79 ] and Y . ruckeri [ 80 ].…”

Section: Resultsmentioning

confidence: 99%

Allelic variation of Escherichia coli outer membrane protein A: Impact on cell surface properties, stress tolerance and allele distribution

Liao

Santoscoy²,

Craft³

et al. 2022

PLoS ONE

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“…Early studies showed the outer membrane protein A (OmpA) was not only a common phage receptor of Enterobacteriaceae 23 31 , but also a common immune protective antigen beyond the V antigen conserved within Yersinia species 32 33 . The phage Yep-phi, required not only LPS but also Ail and OmpF for its efficient infection, losing of the ail and ompF products may resulted in defective phage receptors 34 .…”

Section: Resultsmentioning

confidence: 99%

“…Many studies showed that OmpA was a common phage receptor of Enterobacteriaceae 23 31 , but also a common immune protective antigen beyond the V antigen conserved within Yersinia species 32 33 . Unlike Y. pseudotuberculosis and Y. enterocolitica , Y. pestis had a rough LPS without O antigen where Ail and OmpF were identified to act as the receptors of Yep-phi in addition to the rough lipopolysaccharide of Y. pestis 34 .…”

Section: Discussionmentioning

confidence: 99%

DTDP-rhamnosyl transferase RfbF, is a newfound receptor-related regulatory protein for phage phiYe-F10 specific for Yersinia enterocolitica serotype O:3

Liang

Zha

et al. 2016

Sci Rep

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Bacteriophages and their hosts are continuously engaged in evolutionary competition. Here we isolated a lytic phage phiYe-F10 specific for Yersinia enterocolitica serotype O:3. We firstly described the phage receptor was regulated by DTDP-rhamnosyl transferase RfbF, encoded within the rfb cluster that was responsible for the biosynthesis of the O antigens. The deletion of DTDP-rhamnosyl transferase RfbF of wild type O:3 strain caused failure in phiYe-F10 adsorption; however, the mutation strain retained agglutination with O:3 antiserum; and complementation of its mutant converted its sensitivity to phiYe-F10. Therefore, DTDP-rhamnosyl transferase RfbF was responsible for the phage infection but did not affect recognition of Y. enterocolitica O:3 antiserum. Further, the deletions in the putative O-antigen biosynthesis protein precursor and outer membrane protein had no effect on sensitivity to phiYe-F10 infection. However, adsorption of phages onto mutant HNF10-ΔO-antigen took longer time than onto the WT, suggesting that deletion of the putative O-antigen biosynthesis protein precursor reduced the infection efficiency.

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“…Perhaps, this determines the fact that the structure of the population of H. pylori strains circulating in Russia is practically not studied today. At the same time, the INDEL-typing method [10][11][12][13], based on the available PCR method, is widely used to study the phylogenetic relationships between strains of some other microorganisms, in addition to the MLST typing method. To date, the method of INDEL-typing of H. pylori strains was not been described in the world literature.…”

Section: Introductionmentioning

confidence: 99%

New tool for phylogenetic analysis of Helicobacter pylori

Сорокин¹,

Vjacheslavovich²,

Sergeevich³

et al. 2020

World J. Adv. Res. Rev.

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The aim of this study was to detect canonical insertion/deletion (INDEL) markers in the genome of Helicobacter pylori and offer INDEL-typing method for differentiation of H. pylori strains. For comparative analysis of the genomes of H. pylori presented in the GenBank database, a local database of nucleotide sequences of 69 H. pylori strains was created. For detecting all INDEL markers with a preset size of 6-20 bp a pairwise comparison of more than 1500 open reading frames (ORF) in the genomes of local database strains was performed. Ten loci containing INDEL markers were founded. The five most variable loci were tested in silico with 21 strains with known geographical origin from the most common populations of hpEurope, hspWAfrica, and hspEAsia. Fifteen individual genotypes with a high diversity index (DI=0.95) were identified. For cluster analysis, the minimal spanning tree (MST) method was used, which demonstrated a clear distribution of clusters according to the geographical origin of the strains tested. INDEL-typing of 21 regional strains from the Astrakhan region was performed in vitro. It was shown that an extensive majority of them belong to the population hpEurope. The findings in this study indicate that the proposed INDEL-typing method almost perfectly reflects the geographical distribution of H. pylori strains determined by the multilocus sequence typing (MLST) method, despite the fact that the primary object of research is completely different genes. Further research is needed to determine the geographical origin of H. pylori strains in Russia.

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Gene polymorphism analysis of Yersinia enterocoliticaouter membrane protein A and putative outer membrane protein A family protein

Cited by 6 publications

References 35 publications

Allelic variation of Escherichia coli outer membrane protein A: Impact on cell surface properties, stress tolerance and allele distribution

Allelic variation of Escherichia coli outer membrane protein A: Impact on cell surface properties, stress tolerance and allele distribution

DTDP-rhamnosyl transferase RfbF, is a newfound receptor-related regulatory protein for phage phiYe-F10 specific for Yersinia enterocolitica serotype O:3

New tool for phylogenetic analysis of Helicobacter pylori

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