Analysis of the Presence ofail,ystAandystBGenes inYersinia enterocoliticaStrains Isolated from Aborting Sows and Aborted Fetuses

Platt-Samoraj, A.; Ugorski, Maciej; Szweda, W.; Szczerba‐Turek, Anna; Wojciech, K.; Procajło, Z.

doi:10.1111/j.1439-0450.2006.00969.x

Cited by 35 publications

(26 citation statements)

References 32 publications

(67 reference statements)

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“…A lower prevalence has been reported in Italy (13%), Greece (13%), and Poland (4%) (Bonardi et al, 2003;Kechagia et al, 2007;Kot et al, 2007). Y. enterocolitica 4=O:3 was the only bioserotype found in the present study and has also been shown to be the most common type in slaughtered pigs in Finland, Germany, Greece, Italy, Norway, Switzerland, and Poland (Fredriksson-Ahomaa et al, 2000Bonardi et al, 2003;Gü rtler et al, 2005;Nesbakken et al, 2006;Platt-Samoraj et al, 2006;Kechagia et al, 2007). However, bioserotype 3=O:5,27 dominates in slaughter pigs in the United Kingdom (Milnes et al, 2008).…”

Section: Discussioncontrasting

confidence: 52%

Prevalence of EnteropathogenicYersiniain Estonian, Latvian, and Russian (Leningrad Region) Pigs

Martínez

Fredriksson-Ahomaa

Sokolova³

et al. 2009

Foodborne Pathogens and Disease

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Tonsils of 457 fattening pigs from Estonia (n = 151), Latvia (n = 109), and the Leningrad Region of Russia (n = 197) were collected between 2004 and 2007 to study the prevalence of enteropathogenic Yersinia in slaughter pigs. Yersinia enterocolitica and Yersinia pseudotuberculosis were isolated by selective and cold enrichment. Pathogenic Y. enterocolitica and Y. pseudotuberculosis were identified by PCR targeting the chromosomal genes ail and inv, respectively. The presence of the virulence plasmid was confirmed by PCR targeting the virF gene of Y. enterocolitica and Y. pseudotuberculosis. The prevalence of ail-positive Y. enterocolitica was 89% in Estonia, 64% in Latvia, and 34% in Russia, with 81% of ail-positive samples being virF-positive. A statistically significant (p < 0.05) difference between Estonian and Latvian pigs and between pigs from Latvia and the Leningrad Region of Russia was observed when assuming randomized sampling. Y. enterocolitica bioserotype 4/O:3 was the only pathogenic type found. The prevalence of inv and virF-positive Y. pseudotuberculosis was higher in Russia (7%) than in Latvia (5%) and Estonia (1%), with a statistically significant difference between pigs from Estonia and the Leningrad Region of Russia when assuming both randomized sampling and clustering at farms. All Y. pseudotuberculosis isolates were bioserotype 2/O:3. A total of eight pigs (2%), one pig from Latvia and seven pigs from the Leningrad Region of Russia, carried both pathogenic Y. enterocolitica and Y. pseudotuberculosis in the tonsils. Cold enrichment was found to be a more efficient method compared to selective enrichment to isolate both species. Pigs seem to be an important reservoir of human enteropathogenic Y. enterocolitica 4/O:3 and Y. pseudotuberculosis 2/O:3 in these countries.

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Section: Discussioncontrasting

confidence: 52%

Prevalence of EnteropathogenicYersiniain Estonian, Latvian, and Russian (Leningrad Region) Pigs

Martínez

Fredriksson-Ahomaa

Sokolova³

et al. 2009

Foodborne Pathogens and Disease

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“…In contrast with the studies of pathogenic strains of Y. enterocolitica O:3 by Thoerner et al (2003) and Platt-Samoraj et al (2006) where the ystA gene was identified in 100% and 99%, respectively, in our study the ystA gene was found in only 83% of isolates. A study of reference strains showed the The yadA and virF genes were identified in 79% and 49% of isolates of Y. enterocolitica O:3, respectively, the yadA gene was found only in pathogenic reference strain of Y. enterocolitica CCM 5671, and the virF gene was not identified in any of the reference strains.…”

Section: Resultscontrasting

confidence: 99%

“…The ail gene encodes Ail protein, it occurs only in the pathogenic strains of Y. enterocolitica. This protein participates in bacterial adhesion to mammalian cells, facilitates their invasion into eukaryotic cells, and intensifies their resistance to the bacteriocidal effects of complement (Platt-Samoraj et al 2006). The rfbC gene is localised on the chromosome only in pathogenic strains of Y. enterocolitica serotype O:3 (Weynants et al 1996).…”

mentioning

confidence: 99%

“…Also important for the pathogenicity reasons is the Yst thermostable enterotoxin, which is a protein with the molecular weight of 7494 Da. The enterotoxin is encoded by the yst gene, and facilitates the invasion of the microorganism into tissues by damaging the intestinal epithelium (Platt-Samoraj et al 2006). Pathogenic strains of Y. enterocolitica produce the thermostable enterotoxin YstA (ystA gene), which, sporadically, may also be produced by non-pathogenic strains of Y. intermedia and Y. enterocolitica of the biotype 1A (Thoerner et al 2003;Singh & Virdi 2004;Platt-Samoraj et al 2006).…”

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confidence: 99%

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Detection of pathogenic Yersinia enterocolitica serotype O:3 by biochemical, serological, and PCR methods

Simonova¹,

Vazlerova²,

Steinhauserova³

2007

Czech J. Food Sci.

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In this study, the pathogenic Y. enterocolitica of serotype O:3 was monitored. The serotype is widely spread in Europe and has been linked to human yersiniosis. For the detection of pathogenic strains were used biochemical and serological methods as well as PCR methods based on the identification of virulence genes (ail, rfbC, ystA, yadA, virF). The occurrence of Y. enterocolitica O:3 strains was monitored in slaughter animals from a number of farms in the Czech Republic. A total of 3748 samples were collected coming from pigs (1388), cattle (633), poultry (902), and slaughter facilities (825). Fifty-two Y. enterocolitica O:3 isolates were identified by biochemical and serologic methods, and 53 Y. enterocolitica O:3 isolates were identified by PCR methods (46 isolates from pigs, 2 isolates from poultry, 3 isolates from cattle, and 2 isolates from a poultry slaughtering facility). All isolates of Y. enterocolitica O:3 carried genes ail and rfbC, 83% isolates carried gene ystA, 79% isolates carried gene yadA and 49% isolates carried gene virF. The use of PCR methods based on the identification of ail and rfbC genes provides for a sufficiently specific identification of pathogenic Y. enterocolitica O:3 strains with optimum time consumption compared to biochemical and serological methods. It is not recommendable to use other PCR methods (detection of the ystA, yadA, and virF genes) for the detection of pathogenic Y. enterocolitica strains because those methods are not very specific for the determination of pathogenicity.

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“…None of the examined isolates was ystB-positive. Platt-Samoraj et al (15) found the presence of ail, ystA, and ystB virulence markers in Y. enterocolitica strains isolated from aborting sows and aborted foetuses. Among 45 Y. enterocolitica isolates, 40, belonging to biotype 1A, contained only the ystB gene, whereas the remaining five, belonging to bioserotype 4/O:3, carried ail and ystA genes.…”

Section: Discussionmentioning

confidence: 99%

Genotypic virulence markers of Yersinia enterocolitica O:9 isolated from pigs and wild boars serologically positive and negative for brucellosis

Weiner

Kubajka

Szulowski

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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The paper concerns molecular study on pathogenicity markers of fourteen Y. enterocolitica O:9 isolated from pigs in which initially positive serological reactions for brucellosis were observed (n = 41), healthy pigs, which were brucellosis-negative (n = 258), and wild boars serologically negative for brucellosis (n = 209). PCR identification proved that all isolates were ail, ystA-and myfA-positive. The plasmid encoding yadA marker was detected in nine isolates that originated from pigs serologically positive or negative for brucellosis, and from one isolate of wild boar origin. Furthermore, none of the examined isolates was ystB-positive. Results of the investigations indicate that the Y. enterocolitica O:9 isolates from pigs or wild boars, regardless of whether they were serologically positive or negative for brucellosis, may also be potentially pathogenic for humans, due to the presence of chromosomal and plasmid encoded molecular markers.

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Analysis of the Presence ofail,ystAandystBGenes inYersinia enterocoliticaStrains Isolated from Aborting Sows and Aborted Fetuses

Cited by 35 publications

References 32 publications

Prevalence of EnteropathogenicYersiniain Estonian, Latvian, and Russian (Leningrad Region) Pigs

Prevalence of EnteropathogenicYersiniain Estonian, Latvian, and Russian (Leningrad Region) Pigs

Detection of pathogenic Yersinia enterocolitica serotype O:3 by biochemical, serological, and PCR methods

Genotypic virulence markers of Yersinia enterocolitica O:9 isolated from pigs and wild boars serologically positive and negative for brucellosis

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