Genotypic virulence markers of Yersinia enterocolitica O:9 isolated from pigs and wild boars serologically positive and negative for brucellosis

Abstract

The paper concerns molecular study on pathogenicity markers of fourteen Y. enterocolitica O:9 isolated from pigs in which initially positive serological reactions for brucellosis were observed (n = 41), healthy pigs, which were brucellosis-negative (n = 258), and wild boars serologically negative for brucellosis (n = 209). PCR identification proved that all isolates were ail, ystA-and myfA-positive. The plasmid encoding yadA marker was detected in nine isolates that originated from pigs serologically positive or negative for brucellosis, and from one isolate of wild boar origin. Furthermore, none of the examined isolates was ystB-positive. Results of the investigations indicate that the Y. enterocolitica O:9 isolates from pigs or wild boars, regardless of whether they were serologically positive or negative for brucellosis, may also be potentially pathogenic for humans, due to the presence of chromosomal and plasmid encoded molecular markers.

Prevalence, Virulence and Antimicrobial Susceptibility of Salmonella spp., Yersinia enterocolitica and Listeria monocytogenes in European Wild Boar (Sus scrofa) Hunted in Tuscany (Central Italy)

“…Pathogenic bacteria belonging to this species were found in wild boar specimens, as well as non-pathogenic ones, strengthening the idea, suggested by some studies, that this animal could act as an accidental Yersinia carrier [43,60]. In this investigation, the ystA virulence gene was the most prevalent, as demonstrated for other wild boar isolates [62]. Moreover, several wild boar isolates scored positive for the inv [60], ail [59,62,63] and ystB [58,61] virulence genes, as obtained from samples here investigated.…”

“…In this investigation, the ystA virulence gene was the most prevalent, as demonstrated for other wild boar isolates [62]. Moreover, several wild boar isolates scored positive for the inv [60], ail [59,62,63] and ystB [58,61] virulence genes, as obtained from samples here investigated. virF is a plasmid regulatory gene located on the virulence plasmid designated pYV; the plasmid genes encoded by this plasmid guide the invasion of Yersinia enterocolitica and enable bacteria to survive inside the human host, and for this reasons they are considered essential for pathogenesis [64].…”

Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders

“…enterocolitica . Furthermore, bioserotype 2/O:5,27 has been isolated from a variety of animals, such as cattle, pigs, hares and wild boars, and no primary reservoir has been identified yet [ 34 ; 35 ]. Bioserotype 3/O:1,2,3, alternatively called the “chinchilla-type” [ 36 ], has also previously been isolated from pigs [ 37 ].…”